Quantitative aerobic physiology of the yeast Dekkera bruxellensis, a major contaminant in bioethanol production plants
Dekkera bruxellensis has been described as the major contaminant yeast of industrial ethanol production, although little is known about its physiology. The aim of this study was to investigate the growth of this yeast in diverse carbon sources and involved conducting shake-flask and glucose- or sucr...
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| Veröffentlicht in: | FEMS yeast research 2013-02, Vol.13 (1), p.34-43 |
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| creator | Leite, Fernanda Cristina Bezerra Basso, Thiago Olitta Pita, Will de Barros Gombert, Andreas Karoly Simões, Diogo Ardaillon de Morais, Jr, Marcos Antonio |
| description | Dekkera bruxellensis has been described as the major contaminant yeast of industrial ethanol production, although little is known about its physiology. The aim of this study was to investigate the growth of this yeast in diverse carbon sources and involved conducting shake-flask and glucose- or sucrose-limited chemostats experiments, and from the chemostat data, the stoichiometry of biomass formation during aerobic growth was established. As a result of the shake-flask experiments with hexoses or disaccharides, the specific growth rates were calculated, and a different behavior in rich and mineral medium was observed concerning to profile of acetate and ethanol production. In C-limited chemostats conditions, the metabolism of this yeast was completely respiratory, and the biomass yields reached values of 0.62 gDW gS(-1) . In addition, glucose pulses were applied to the glucose- or sucrose-limited chemostats. These results showed that D. bruxellensis has a short-term Crabtree effect. While the glucose pulse was at the sucrose-limited chemostat, sucrose accumulated at the reactor, indicating the presence of a glucose repression mechanism in D. bruxellensis. |
| doi_str_mv | 10.1111/1567-1364.12007 |
| format | Article |
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The aim of this study was to investigate the growth of this yeast in diverse carbon sources and involved conducting shake-flask and glucose- or sucrose-limited chemostats experiments, and from the chemostat data, the stoichiometry of biomass formation during aerobic growth was established. As a result of the shake-flask experiments with hexoses or disaccharides, the specific growth rates were calculated, and a different behavior in rich and mineral medium was observed concerning to profile of acetate and ethanol production. In C-limited chemostats conditions, the metabolism of this yeast was completely respiratory, and the biomass yields reached values of 0.62 gDW gS(-1) . In addition, glucose pulses were applied to the glucose- or sucrose-limited chemostats. These results showed that D. bruxellensis has a short-term Crabtree effect. While the glucose pulse was at the sucrose-limited chemostat, sucrose accumulated at the reactor, indicating the presence of a glucose repression mechanism in D. bruxellensis.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1111/1567-1364.12007</identifier><identifier>PMID: 23078341</identifier><language>eng</language><publisher>England</publisher><subject>Acetates - metabolism ; Aerobiosis ; beta-Fructofuranosidase - metabolism ; Biomass ; Carbon - metabolism ; Cell Respiration ; Culture Media ; Dekkera - growth & development ; Dekkera - metabolism ; Dekkera - physiology ; Ethanol - metabolism ; Fermentation ; Fungal Proteins - metabolism ; Glucose - metabolism ; Industrial Microbiology ; Oxygen - metabolism ; Saccharomyces cerevisiae - chemistry ; Sucrose - metabolism</subject><ispartof>FEMS yeast research, 2013-02, Vol.13 (1), p.34-43</ispartof><rights>2012 Federation of European Microbiological Societies. 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While the glucose pulse was at the sucrose-limited chemostat, sucrose accumulated at the reactor, indicating the presence of a glucose repression mechanism in D. bruxellensis.</description><subject>Acetates - metabolism</subject><subject>Aerobiosis</subject><subject>beta-Fructofuranosidase - metabolism</subject><subject>Biomass</subject><subject>Carbon - metabolism</subject><subject>Cell Respiration</subject><subject>Culture Media</subject><subject>Dekkera - growth & development</subject><subject>Dekkera - metabolism</subject><subject>Dekkera - physiology</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Fungal Proteins - metabolism</subject><subject>Glucose - metabolism</subject><subject>Industrial Microbiology</subject><subject>Oxygen - metabolism</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Sucrose - metabolism</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1PwzAMhiMEYmNw5oZy5MC2pGmb9ojGpzQJIcG5clOXZWubkaQT-_dkbMwXW_brV_ZDyDVnEx5iypNUjrlI4wmPGJMnZHjsnB7rJB2QC-eWjHHJWHZOBpFgMhMxH5LNew-d1x683iAFtKbUiq4XW6dNY7621NTUL5BuEZynD7haoQVa2v4HmwY7p90dBdrC0liqTOeh1V0wpLqjpTboF9CZhq6tqXrltenougljd0nOamgcXh3yiHw-PX7MXsbzt-fX2f18rKJc-nEqlABViiyvRckrYEkVYawyGUdVytIcMHyUQFULXoo8VpILnkY5hH6WocjFiNzufcMF3z06X7TaqXA6dGh6V_BIiiThUsggne6lyhrnLNbF2uoW7LbgrNjBLnY4ix3a4g922Lg5mPdli9VR_09X_AKmaHu1</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Leite, Fernanda Cristina Bezerra</creator><creator>Basso, Thiago Olitta</creator><creator>Pita, Will de Barros</creator><creator>Gombert, Andreas Karoly</creator><creator>Simões, Diogo Ardaillon</creator><creator>de Morais, Jr, Marcos Antonio</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201302</creationdate><title>Quantitative aerobic physiology of the yeast Dekkera bruxellensis, a major contaminant in bioethanol production plants</title><author>Leite, Fernanda Cristina Bezerra ; Basso, Thiago Olitta ; Pita, Will de Barros ; Gombert, Andreas Karoly ; Simões, Diogo Ardaillon ; de Morais, Jr, Marcos Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-63c3acb389f3b1da05d2e4c8742d6069ae1705adf31b394c7131629aae188e393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acetates - metabolism</topic><topic>Aerobiosis</topic><topic>beta-Fructofuranosidase - metabolism</topic><topic>Biomass</topic><topic>Carbon - metabolism</topic><topic>Cell Respiration</topic><topic>Culture Media</topic><topic>Dekkera - growth & development</topic><topic>Dekkera - metabolism</topic><topic>Dekkera - physiology</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Fungal Proteins - metabolism</topic><topic>Glucose - metabolism</topic><topic>Industrial Microbiology</topic><topic>Oxygen - metabolism</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Sucrose - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leite, Fernanda Cristina Bezerra</creatorcontrib><creatorcontrib>Basso, Thiago Olitta</creatorcontrib><creatorcontrib>Pita, Will de Barros</creatorcontrib><creatorcontrib>Gombert, Andreas Karoly</creatorcontrib><creatorcontrib>Simões, Diogo Ardaillon</creatorcontrib><creatorcontrib>de Morais, Jr, Marcos Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leite, Fernanda Cristina Bezerra</au><au>Basso, Thiago Olitta</au><au>Pita, Will de Barros</au><au>Gombert, Andreas Karoly</au><au>Simões, Diogo Ardaillon</au><au>de Morais, Jr, Marcos Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative aerobic physiology of the yeast Dekkera bruxellensis, a major contaminant in bioethanol production plants</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2013-02</date><risdate>2013</risdate><volume>13</volume><issue>1</issue><spage>34</spage><epage>43</epage><pages>34-43</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>Dekkera bruxellensis has been described as the major contaminant yeast of industrial ethanol production, although little is known about its physiology. The aim of this study was to investigate the growth of this yeast in diverse carbon sources and involved conducting shake-flask and glucose- or sucrose-limited chemostats experiments, and from the chemostat data, the stoichiometry of biomass formation during aerobic growth was established. As a result of the shake-flask experiments with hexoses or disaccharides, the specific growth rates were calculated, and a different behavior in rich and mineral medium was observed concerning to profile of acetate and ethanol production. In C-limited chemostats conditions, the metabolism of this yeast was completely respiratory, and the biomass yields reached values of 0.62 gDW gS(-1) . In addition, glucose pulses were applied to the glucose- or sucrose-limited chemostats. These results showed that D. bruxellensis has a short-term Crabtree effect. While the glucose pulse was at the sucrose-limited chemostat, sucrose accumulated at the reactor, indicating the presence of a glucose repression mechanism in D. bruxellensis.</abstract><cop>England</cop><pmid>23078341</pmid><doi>10.1111/1567-1364.12007</doi><tpages>10</tpages></addata></record> |
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| subjects | Acetates - metabolism Aerobiosis beta-Fructofuranosidase - metabolism Biomass Carbon - metabolism Cell Respiration Culture Media Dekkera - growth & development Dekkera - metabolism Dekkera - physiology Ethanol - metabolism Fermentation Fungal Proteins - metabolism Glucose - metabolism Industrial Microbiology Oxygen - metabolism Saccharomyces cerevisiae - chemistry Sucrose - metabolism |
| title | Quantitative aerobic physiology of the yeast Dekkera bruxellensis, a major contaminant in bioethanol production plants |
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