Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness
Abstract Background Congenital cytomegalovirus (CMV) infection is the leading cause of non-genetic congenital hearing loss. The contribution of congenital CMV to prelingual deafness and the pathophysiology is largely unknown. Objective (1) To analyze the prevalence of congenital CMV among cochlear i...
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| creator | de Vries, Jutte J.C Vesseur, Annemarie Rotteveel, Liselotte J.C Korver, Anna M.H Rusman, Lisette G Wessels, Els Kroes, Aloys C.M Mylanus, Emmanuel A Oudesluys-Murphy, Anne M Frijns, Johan H.M Vossen, Ann C.T.M |
| description | Abstract Background Congenital cytomegalovirus (CMV) infection is the leading cause of non-genetic congenital hearing loss. The contribution of congenital CMV to prelingual deafness and the pathophysiology is largely unknown. Objective (1) To analyze the prevalence of congenital CMV among cochlear implant (CI) recipients with prelingual deafness. (2) To genotype CMV present in dried blood spots (DBS) and in the inner ear years after birth. Study design Children and adults with prelingual deafness who received a CI in 2010–2011 were included prospectively. Perilymphatic fluids were collected during CI surgery and, in the pediatric cases, DBS were retrieved for CMV DNA detection. Furthermore, a cohort of children with prelingual deafness who received a CI between 2003 and 2008 were included retrospectively. CMV detection in DBS and perilymph was followed by gB and gH genotyping. Results Seventysix pediatric CI recipients were included. Seventy DBS were tested for CMV DNA, resulting in a prevalence of congenital CMV of 14% (10/70). Perilymphatic fluid was available from 29 pediatric CI recipients. One perilymph fluid, of a 21-month old girl with congenital CMV, asymptomatic at birth, was CMV DNA positive. The CMV strain in the perilymph was genotypically identical to the strain present in her DBS (gB1/gH2). Perilymph samples from 21 adult CI recipients were CMV DNA negative. Conclusions Our study stresses the important contribution of congenital CMV among pediatric CI recipients. Furthermore, our genotyping data support the hypothesis that CMV-related hearing loss is associated with ongoing viral replication in the inner ear up to years after birth. |
| doi_str_mv | 10.1016/j.jcv.2012.10.008 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1273506711</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1386653212003897</els_id><sourcerecordid>1273506711</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-36e6c9e0562b303934a8ccbd7808f92c4023b591d7ac6f5027bcca24d0d24cca3</originalsourceid><addsrcrecordid>eNqNks2KFDEQgBtR3HX1AbxILoKXHvPTnaQRhGX8hUUP6jmkk_ROxnSnTdIj8yi-rdXMqOBBPCWpfFUV6ktVPSZ4QzDhz_ebvTlsKCYUzhuM5Z3qkkjB6rbj4i7smeQ1bxm9qB7kvMeYtKwR96sLykhDOoYvqx_bY4mju9UhHnxaMnr14RpZV5wpPk7IT8gm7yzqQ4wW5TmWjPRk0eySD8dx3uniDRrC4m1GQ4oj3FivS4Loymm7hIJMNLvgdEJ-nIOeCkrO-Nm7Cap992WH5uSCn24XHaC5HiaX88Pq3qBDdo_O61X15c3rz9t39c3Ht--31ze1aQQpNeOOm87hltOeYdaxRktjeisklkNHTYMp69uOWKENH1pMRW-Mpo3FljawY1fVs1PdOcVvi8tFjT4bF-CdLi5ZESpYi7kg5H9QgBspKaDkhJoUc05uUHPyo05HRbBa5am9AnlqlbeGQB7kPDmXX_rR2d8Zv2wB8PQM6Gx0GJKejM9_OC4ZllwA9-LEOZjbwbuksoFhGzADgy_KRv_PZ7z8K9uAGw8Nv7qjy_u4pAmEKKIyVVh9Wn_Z-skIxZjJTrCfet_O6Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1272734882</pqid></control><display><type>article</type><title>Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>de Vries, Jutte J.C ; Vesseur, Annemarie ; Rotteveel, Liselotte J.C ; Korver, Anna M.H ; Rusman, Lisette G ; Wessels, Els ; Kroes, Aloys C.M ; Mylanus, Emmanuel A ; Oudesluys-Murphy, Anne M ; Frijns, Johan H.M ; Vossen, Ann C.T.M</creator><creatorcontrib>de Vries, Jutte J.C ; Vesseur, Annemarie ; Rotteveel, Liselotte J.C ; Korver, Anna M.H ; Rusman, Lisette G ; Wessels, Els ; Kroes, Aloys C.M ; Mylanus, Emmanuel A ; Oudesluys-Murphy, Anne M ; Frijns, Johan H.M ; Vossen, Ann C.T.M</creatorcontrib><description>Abstract Background Congenital cytomegalovirus (CMV) infection is the leading cause of non-genetic congenital hearing loss. The contribution of congenital CMV to prelingual deafness and the pathophysiology is largely unknown. Objective (1) To analyze the prevalence of congenital CMV among cochlear implant (CI) recipients with prelingual deafness. (2) To genotype CMV present in dried blood spots (DBS) and in the inner ear years after birth. Study design Children and adults with prelingual deafness who received a CI in 2010–2011 were included prospectively. Perilymphatic fluids were collected during CI surgery and, in the pediatric cases, DBS were retrieved for CMV DNA detection. Furthermore, a cohort of children with prelingual deafness who received a CI between 2003 and 2008 were included retrospectively. CMV detection in DBS and perilymph was followed by gB and gH genotyping. Results Seventysix pediatric CI recipients were included. Seventy DBS were tested for CMV DNA, resulting in a prevalence of congenital CMV of 14% (10/70). Perilymphatic fluid was available from 29 pediatric CI recipients. One perilymph fluid, of a 21-month old girl with congenital CMV, asymptomatic at birth, was CMV DNA positive. The CMV strain in the perilymph was genotypically identical to the strain present in her DBS (gB1/gH2). Perilymph samples from 21 adult CI recipients were CMV DNA negative. Conclusions Our study stresses the important contribution of congenital CMV among pediatric CI recipients. Furthermore, our genotyping data support the hypothesis that CMV-related hearing loss is associated with ongoing viral replication in the inner ear up to years after birth.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2012.10.008</identifier><identifier>PMID: 23141930</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Allergy and Immunology ; Biological and medical sciences ; Birth ; Blood - virology ; Child ; Child, Preschool ; Cochlear implant ; Cochlear Implants ; Congenital cytomegalovirus ; Cytomegalovirus ; Cytomegalovirus - classification ; Cytomegalovirus - genetics ; Cytomegalovirus - isolation & purification ; Cytomegalovirus Infections - congenital ; Cytomegalovirus Infections - virology ; Deafness - etiology ; Deafness - virology ; Desiccation ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Female ; Fundamental and applied biological sciences. Psychology ; Genotype ; Human viral diseases ; Humans ; Infant ; Infant, Newborn ; Infectious Disease ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Perilymph - virology ; Specimen Handling - methods ; Viral diseases ; Virology ; Young Adult</subject><ispartof>Journal of clinical virology, 2013-02, Vol.56 (2), p.113-117</ispartof><rights>Elsevier B.V.</rights><rights>2012 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-36e6c9e0562b303934a8ccbd7808f92c4023b591d7ac6f5027bcca24d0d24cca3</citedby><cites>FETCH-LOGICAL-c471t-36e6c9e0562b303934a8ccbd7808f92c4023b591d7ac6f5027bcca24d0d24cca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1386653212003897$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26830867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23141930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Vries, Jutte J.C</creatorcontrib><creatorcontrib>Vesseur, Annemarie</creatorcontrib><creatorcontrib>Rotteveel, Liselotte J.C</creatorcontrib><creatorcontrib>Korver, Anna M.H</creatorcontrib><creatorcontrib>Rusman, Lisette G</creatorcontrib><creatorcontrib>Wessels, Els</creatorcontrib><creatorcontrib>Kroes, Aloys C.M</creatorcontrib><creatorcontrib>Mylanus, Emmanuel A</creatorcontrib><creatorcontrib>Oudesluys-Murphy, Anne M</creatorcontrib><creatorcontrib>Frijns, Johan H.M</creatorcontrib><creatorcontrib>Vossen, Ann C.T.M</creatorcontrib><title>Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Abstract Background Congenital cytomegalovirus (CMV) infection is the leading cause of non-genetic congenital hearing loss. The contribution of congenital CMV to prelingual deafness and the pathophysiology is largely unknown. Objective (1) To analyze the prevalence of congenital CMV among cochlear implant (CI) recipients with prelingual deafness. (2) To genotype CMV present in dried blood spots (DBS) and in the inner ear years after birth. Study design Children and adults with prelingual deafness who received a CI in 2010–2011 were included prospectively. Perilymphatic fluids were collected during CI surgery and, in the pediatric cases, DBS were retrieved for CMV DNA detection. Furthermore, a cohort of children with prelingual deafness who received a CI between 2003 and 2008 were included retrospectively. CMV detection in DBS and perilymph was followed by gB and gH genotyping. Results Seventysix pediatric CI recipients were included. Seventy DBS were tested for CMV DNA, resulting in a prevalence of congenital CMV of 14% (10/70). Perilymphatic fluid was available from 29 pediatric CI recipients. One perilymph fluid, of a 21-month old girl with congenital CMV, asymptomatic at birth, was CMV DNA positive. The CMV strain in the perilymph was genotypically identical to the strain present in her DBS (gB1/gH2). Perilymph samples from 21 adult CI recipients were CMV DNA negative. Conclusions Our study stresses the important contribution of congenital CMV among pediatric CI recipients. Furthermore, our genotyping data support the hypothesis that CMV-related hearing loss is associated with ongoing viral replication in the inner ear up to years after birth.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Allergy and Immunology</subject><subject>Biological and medical sciences</subject><subject>Birth</subject><subject>Blood - virology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cochlear implant</subject><subject>Cochlear Implants</subject><subject>Congenital cytomegalovirus</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - classification</subject><subject>Cytomegalovirus - genetics</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus Infections - congenital</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Deafness - etiology</subject><subject>Deafness - virology</subject><subject>Desiccation</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Perilymph - virology</subject><subject>Specimen Handling - methods</subject><subject>Viral diseases</subject><subject>Virology</subject><subject>Young Adult</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2KFDEQgBtR3HX1AbxILoKXHvPTnaQRhGX8hUUP6jmkk_ROxnSnTdIj8yi-rdXMqOBBPCWpfFUV6ktVPSZ4QzDhz_ebvTlsKCYUzhuM5Z3qkkjB6rbj4i7smeQ1bxm9qB7kvMeYtKwR96sLykhDOoYvqx_bY4mju9UhHnxaMnr14RpZV5wpPk7IT8gm7yzqQ4wW5TmWjPRk0eySD8dx3uniDRrC4m1GQ4oj3FivS4Loymm7hIJMNLvgdEJ-nIOeCkrO-Nm7Cap992WH5uSCn24XHaC5HiaX88Pq3qBDdo_O61X15c3rz9t39c3Ht--31ze1aQQpNeOOm87hltOeYdaxRktjeisklkNHTYMp69uOWKENH1pMRW-Mpo3FljawY1fVs1PdOcVvi8tFjT4bF-CdLi5ZESpYi7kg5H9QgBspKaDkhJoUc05uUHPyo05HRbBa5am9AnlqlbeGQB7kPDmXX_rR2d8Zv2wB8PQM6Gx0GJKejM9_OC4ZllwA9-LEOZjbwbuksoFhGzADgy_KRv_PZ7z8K9uAGw8Nv7qjy_u4pAmEKKIyVVh9Wn_Z-skIxZjJTrCfet_O6Q</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>de Vries, Jutte J.C</creator><creator>Vesseur, Annemarie</creator><creator>Rotteveel, Liselotte J.C</creator><creator>Korver, Anna M.H</creator><creator>Rusman, Lisette G</creator><creator>Wessels, Els</creator><creator>Kroes, Aloys C.M</creator><creator>Mylanus, Emmanuel A</creator><creator>Oudesluys-Murphy, Anne M</creator><creator>Frijns, Johan H.M</creator><creator>Vossen, Ann C.T.M</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness</title><author>de Vries, Jutte J.C ; Vesseur, Annemarie ; Rotteveel, Liselotte J.C ; Korver, Anna M.H ; Rusman, Lisette G ; Wessels, Els ; Kroes, Aloys C.M ; Mylanus, Emmanuel A ; Oudesluys-Murphy, Anne M ; Frijns, Johan H.M ; Vossen, Ann C.T.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-36e6c9e0562b303934a8ccbd7808f92c4023b591d7ac6f5027bcca24d0d24cca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Allergy and Immunology</topic><topic>Biological and medical sciences</topic><topic>Birth</topic><topic>Blood - virology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cochlear implant</topic><topic>Cochlear Implants</topic><topic>Congenital cytomegalovirus</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - classification</topic><topic>Cytomegalovirus - genetics</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>Cytomegalovirus Infections - congenital</topic><topic>Cytomegalovirus Infections - virology</topic><topic>Deafness - etiology</topic><topic>Deafness - virology</topic><topic>Desiccation</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Perilymph - virology</topic><topic>Specimen Handling - methods</topic><topic>Viral diseases</topic><topic>Virology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Vries, Jutte J.C</creatorcontrib><creatorcontrib>Vesseur, Annemarie</creatorcontrib><creatorcontrib>Rotteveel, Liselotte J.C</creatorcontrib><creatorcontrib>Korver, Anna M.H</creatorcontrib><creatorcontrib>Rusman, Lisette G</creatorcontrib><creatorcontrib>Wessels, Els</creatorcontrib><creatorcontrib>Kroes, Aloys C.M</creatorcontrib><creatorcontrib>Mylanus, Emmanuel A</creatorcontrib><creatorcontrib>Oudesluys-Murphy, Anne M</creatorcontrib><creatorcontrib>Frijns, Johan H.M</creatorcontrib><creatorcontrib>Vossen, Ann C.T.M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Vries, Jutte J.C</au><au>Vesseur, Annemarie</au><au>Rotteveel, Liselotte J.C</au><au>Korver, Anna M.H</au><au>Rusman, Lisette G</au><au>Wessels, Els</au><au>Kroes, Aloys C.M</au><au>Mylanus, Emmanuel A</au><au>Oudesluys-Murphy, Anne M</au><au>Frijns, Johan H.M</au><au>Vossen, Ann C.T.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>56</volume><issue>2</issue><spage>113</spage><epage>117</epage><pages>113-117</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Abstract Background Congenital cytomegalovirus (CMV) infection is the leading cause of non-genetic congenital hearing loss. The contribution of congenital CMV to prelingual deafness and the pathophysiology is largely unknown. Objective (1) To analyze the prevalence of congenital CMV among cochlear implant (CI) recipients with prelingual deafness. (2) To genotype CMV present in dried blood spots (DBS) and in the inner ear years after birth. Study design Children and adults with prelingual deafness who received a CI in 2010–2011 were included prospectively. Perilymphatic fluids were collected during CI surgery and, in the pediatric cases, DBS were retrieved for CMV DNA detection. Furthermore, a cohort of children with prelingual deafness who received a CI between 2003 and 2008 were included retrospectively. CMV detection in DBS and perilymph was followed by gB and gH genotyping. Results Seventysix pediatric CI recipients were included. Seventy DBS were tested for CMV DNA, resulting in a prevalence of congenital CMV of 14% (10/70). Perilymphatic fluid was available from 29 pediatric CI recipients. One perilymph fluid, of a 21-month old girl with congenital CMV, asymptomatic at birth, was CMV DNA positive. The CMV strain in the perilymph was genotypically identical to the strain present in her DBS (gB1/gH2). Perilymph samples from 21 adult CI recipients were CMV DNA negative. Conclusions Our study stresses the important contribution of congenital CMV among pediatric CI recipients. Furthermore, our genotyping data support the hypothesis that CMV-related hearing loss is associated with ongoing viral replication in the inner ear up to years after birth.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23141930</pmid><doi>10.1016/j.jcv.2012.10.008</doi><tpages>5</tpages></addata></record> |
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| subjects | Adolescent Adult Allergy and Immunology Biological and medical sciences Birth Blood - virology Child Child, Preschool Cochlear implant Cochlear Implants Congenital cytomegalovirus Cytomegalovirus Cytomegalovirus - classification Cytomegalovirus - genetics Cytomegalovirus - isolation & purification Cytomegalovirus Infections - congenital Cytomegalovirus Infections - virology Deafness - etiology Deafness - virology Desiccation DNA, Viral - genetics DNA, Viral - isolation & purification Female Fundamental and applied biological sciences. Psychology Genotype Human viral diseases Humans Infant Infant, Newborn Infectious Disease Infectious diseases Male Medical sciences Microbiology Middle Aged Miscellaneous Perilymph - virology Specimen Handling - methods Viral diseases Virology Young Adult |
| title | Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness |
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