Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma
Background In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR‐650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR‐650 remains unknown in HCC. Methods In this study, we performed...
Gespeichert in:
| Veröffentlicht in: | Journal of surgical oncology 2013-02, Vol.107 (2), p.105-110 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 110 |
|---|---|
| container_issue | 2 |
| container_start_page | 105 |
| container_title | Journal of surgical oncology |
| container_volume | 107 |
| creator | Zeng, Zhao-lin Li, Fu-jun Gao, Feng Sun, Dong-sheng Yao, Lei |
| description | Background
In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR‐650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR‐650 remains unknown in HCC.
Methods
In this study, we performed a comprehensive analysis to examine the miR‐650 expression level in 248 HCC and 120 paracarcinomatous liver (PCL) tissues. The correlations between miR‐650 expression level and the clinicopathological characteristics (HCC tumorigenicity) were evaluated. The role of miR‐650 played in HCC was investigated by Q‐PCR, western blot, and MTT.
Results
We found that miR‐650 expression was significantly increased in HCC patients and significantly associated with the patients' age (P = 0.0019), differentiation capability (P = 0.0108), and also tumor stage (P = 0.0069). Moreover, we compared the expression level of both ING4 and miR‐650 in 122 HCC patients by western blot and real‐time PCR. Statistical result showed a significant negative correlation between them (rs = −0.2011, P = 0.0264). Transfection and MTT test suggested that miR‐650 decreased the expression of ING4 and stimulate liver cells proliferation significantly.
Conclusion
These data suggested that miR‐650 is correlated with the pathogenesis of HCC and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4. J. Surg. Oncol. 2013;107:105–110. © 2012 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jso.23210 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1273498009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2862762561</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3910-e8df5f4c4ba63927d41fb24a206de3b2a66b03dd412672454b6441f3d269e3c13</originalsourceid><addsrcrecordid>eNp1kc1OGzEUha2KqgTaBS-ALLEpiwn-iz1elqikiRBIoWmXlmfsAacz42DPKOTt6zQhEkhdXene7x4dnQPAGUZDjBC5WkY_JJRg9AEMMJI8k0jmR2CQbiRjQqJjcBLjEiEkJWefwDEhggtG8wFoF6tgH_tad8630FewcfOMjxB0EZY-BJsu1sC1656g8es2e0tP7yYM6tbAVfCPwca43z_Zle58aes6wQGWOpSu9Y3-DD5Wuo72y36egsXN95_jH9nt_WQ6_nablVRilNncVKOKlazQnEoiDMNVQZgmiBtLC6I5LxA1aU24IGzECs4SQg3h0tIS01PwdaebfD33NnaqcXFrR7fW91FhIiiTeQokoRfv0KXvQ5vcJYrnAmPBt4KXO6oMPsZgK7UKrtFhozBS2xJUKkH9KyGx53vFvmisOZCvqSfgagesXW03_1dSs4f7V8ls9-FiZ18OHzr8UVxQMVK_7yaKzn49XM_nSE3oXylLnxo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1268711761</pqid></control><display><type>article</type><title>Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><creator>Zeng, Zhao-lin ; Li, Fu-jun ; Gao, Feng ; Sun, Dong-sheng ; Yao, Lei</creator><creatorcontrib>Zeng, Zhao-lin ; Li, Fu-jun ; Gao, Feng ; Sun, Dong-sheng ; Yao, Lei</creatorcontrib><description>Background
In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR‐650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR‐650 remains unknown in HCC.
Methods
In this study, we performed a comprehensive analysis to examine the miR‐650 expression level in 248 HCC and 120 paracarcinomatous liver (PCL) tissues. The correlations between miR‐650 expression level and the clinicopathological characteristics (HCC tumorigenicity) were evaluated. The role of miR‐650 played in HCC was investigated by Q‐PCR, western blot, and MTT.
Results
We found that miR‐650 expression was significantly increased in HCC patients and significantly associated with the patients' age (P = 0.0019), differentiation capability (P = 0.0108), and also tumor stage (P = 0.0069). Moreover, we compared the expression level of both ING4 and miR‐650 in 122 HCC patients by western blot and real‐time PCR. Statistical result showed a significant negative correlation between them (rs = −0.2011, P = 0.0264). Transfection and MTT test suggested that miR‐650 decreased the expression of ING4 and stimulate liver cells proliferation significantly.
Conclusion
These data suggested that miR‐650 is correlated with the pathogenesis of HCC and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4. J. Surg. Oncol. 2013;107:105–110. © 2012 Wiley Periodicals, Inc.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/jso.23210</identifier><identifier>PMID: 22767438</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Cell Cycle Proteins - metabolism ; Cells, Cultured ; Down-Regulation ; Female ; Genetic Markers ; hepatocellular carcinoma ; Homeodomain Proteins - metabolism ; Humans ; ING4 ; Liver - metabolism ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Male ; MicroRNAs - metabolism ; Middle Aged ; miR-650 ; Neoplasm Staging ; Real-Time Polymerase Chain Reaction ; Tumor Suppressor Proteins - metabolism ; Up-Regulation</subject><ispartof>Journal of surgical oncology, 2013-02, Vol.107 (2), p.105-110</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3910-e8df5f4c4ba63927d41fb24a206de3b2a66b03dd412672454b6441f3d269e3c13</citedby><cites>FETCH-LOGICAL-c3910-e8df5f4c4ba63927d41fb24a206de3b2a66b03dd412672454b6441f3d269e3c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjso.23210$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjso.23210$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22767438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeng, Zhao-lin</creatorcontrib><creatorcontrib>Li, Fu-jun</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Sun, Dong-sheng</creatorcontrib><creatorcontrib>Yao, Lei</creatorcontrib><title>Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>Background
In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR‐650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR‐650 remains unknown in HCC.
Methods
In this study, we performed a comprehensive analysis to examine the miR‐650 expression level in 248 HCC and 120 paracarcinomatous liver (PCL) tissues. The correlations between miR‐650 expression level and the clinicopathological characteristics (HCC tumorigenicity) were evaluated. The role of miR‐650 played in HCC was investigated by Q‐PCR, western blot, and MTT.
Results
We found that miR‐650 expression was significantly increased in HCC patients and significantly associated with the patients' age (P = 0.0019), differentiation capability (P = 0.0108), and also tumor stage (P = 0.0069). Moreover, we compared the expression level of both ING4 and miR‐650 in 122 HCC patients by western blot and real‐time PCR. Statistical result showed a significant negative correlation between them (rs = −0.2011, P = 0.0264). Transfection and MTT test suggested that miR‐650 decreased the expression of ING4 and stimulate liver cells proliferation significantly.
Conclusion
These data suggested that miR‐650 is correlated with the pathogenesis of HCC and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4. J. Surg. Oncol. 2013;107:105–110. © 2012 Wiley Periodicals, Inc.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Genetic Markers</subject><subject>hepatocellular carcinoma</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>ING4</subject><subject>Liver - metabolism</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miR-650</subject><subject>Neoplasm Staging</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Up-Regulation</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1OGzEUha2KqgTaBS-ALLEpiwn-iz1elqikiRBIoWmXlmfsAacz42DPKOTt6zQhEkhdXene7x4dnQPAGUZDjBC5WkY_JJRg9AEMMJI8k0jmR2CQbiRjQqJjcBLjEiEkJWefwDEhggtG8wFoF6tgH_tad8630FewcfOMjxB0EZY-BJsu1sC1656g8es2e0tP7yYM6tbAVfCPwca43z_Zle58aes6wQGWOpSu9Y3-DD5Wuo72y36egsXN95_jH9nt_WQ6_nablVRilNncVKOKlazQnEoiDMNVQZgmiBtLC6I5LxA1aU24IGzECs4SQg3h0tIS01PwdaebfD33NnaqcXFrR7fW91FhIiiTeQokoRfv0KXvQ5vcJYrnAmPBt4KXO6oMPsZgK7UKrtFhozBS2xJUKkH9KyGx53vFvmisOZCvqSfgagesXW03_1dSs4f7V8ls9-FiZ18OHzr8UVxQMVK_7yaKzn49XM_nSE3oXylLnxo</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Zeng, Zhao-lin</creator><creator>Li, Fu-jun</creator><creator>Gao, Feng</creator><creator>Sun, Dong-sheng</creator><creator>Yao, Lei</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma</title><author>Zeng, Zhao-lin ; Li, Fu-jun ; Gao, Feng ; Sun, Dong-sheng ; Yao, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3910-e8df5f4c4ba63927d41fb24a206de3b2a66b03dd412672454b6441f3d269e3c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Carcinoma, Hepatocellular - genetics</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Genetic Markers</topic><topic>hepatocellular carcinoma</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>ING4</topic><topic>Liver - metabolism</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miR-650</topic><topic>Neoplasm Staging</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeng, Zhao-lin</creatorcontrib><creatorcontrib>Li, Fu-jun</creatorcontrib><creatorcontrib>Gao, Feng</creatorcontrib><creatorcontrib>Sun, Dong-sheng</creatorcontrib><creatorcontrib>Yao, Lei</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeng, Zhao-lin</au><au>Li, Fu-jun</au><au>Gao, Feng</au><au>Sun, Dong-sheng</au><au>Yao, Lei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>107</volume><issue>2</issue><spage>105</spage><epage>110</epage><pages>105-110</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><abstract>Background
In the last decade, studies in hepatocellular carcinoma (HCC) demonstrate dysregulation of miRNAs expression. For instance, miR‐650 has been implicated in gastric and colorectal cancer tumorigenicity; however, the role of miR‐650 remains unknown in HCC.
Methods
In this study, we performed a comprehensive analysis to examine the miR‐650 expression level in 248 HCC and 120 paracarcinomatous liver (PCL) tissues. The correlations between miR‐650 expression level and the clinicopathological characteristics (HCC tumorigenicity) were evaluated. The role of miR‐650 played in HCC was investigated by Q‐PCR, western blot, and MTT.
Results
We found that miR‐650 expression was significantly increased in HCC patients and significantly associated with the patients' age (P = 0.0019), differentiation capability (P = 0.0108), and also tumor stage (P = 0.0069). Moreover, we compared the expression level of both ING4 and miR‐650 in 122 HCC patients by western blot and real‐time PCR. Statistical result showed a significant negative correlation between them (rs = −0.2011, P = 0.0264). Transfection and MTT test suggested that miR‐650 decreased the expression of ING4 and stimulate liver cells proliferation significantly.
Conclusion
These data suggested that miR‐650 is correlated with the pathogenesis of HCC and is involved in the HCC tumorigenesis process by inhibiting the expression of ING4. J. Surg. Oncol. 2013;107:105–110. © 2012 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22767438</pmid><doi>10.1002/jso.23210</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4790 |
| ispartof | Journal of surgical oncology, 2013-02, Vol.107 (2), p.105-110 |
| issn | 0022-4790 1096-9098 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1273498009 |
| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Adult Aged Aged, 80 and over Biomarkers, Tumor - metabolism Blotting, Western Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Cell Cycle Proteins - metabolism Cells, Cultured Down-Regulation Female Genetic Markers hepatocellular carcinoma Homeodomain Proteins - metabolism Humans ING4 Liver - metabolism Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Male MicroRNAs - metabolism Middle Aged miR-650 Neoplasm Staging Real-Time Polymerase Chain Reaction Tumor Suppressor Proteins - metabolism Up-Regulation |
| title | Upregulation of miR-650 is correlated with down-regulation of ING4 and progression of hepatocellular carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T15%3A54%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Upregulation%20of%20miR-650%20is%20correlated%20with%20down-regulation%20of%20ING4%20and%20progression%20of%20hepatocellular%20carcinoma&rft.jtitle=Journal%20of%20surgical%20oncology&rft.au=Zeng,%20Zhao-lin&rft.date=2013-02-01&rft.volume=107&rft.issue=2&rft.spage=105&rft.epage=110&rft.pages=105-110&rft.issn=0022-4790&rft.eissn=1096-9098&rft_id=info:doi/10.1002/jso.23210&rft_dat=%3Cproquest_cross%3E2862762561%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1268711761&rft_id=info:pmid/22767438&rfr_iscdi=true |