Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact?
In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage‐gated sodium current (INa) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non‐inactivating “leak” current (Ileak), which was attributed to...
Gespeichert in:
| Veröffentlicht in: | Bioelectromagnetics 2013-02, Vol.34 (2), p.162-164 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 164 |
|---|---|
| container_issue | 2 |
| container_start_page | 162 |
| container_title | Bioelectromagnetics |
| container_volume | 34 |
| creator | Verkerk, Arie O. van Ginneken, Antoni C.G. Wilders, Ronald |
| description | In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage‐gated sodium current (INa) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non‐inactivating “leak” current (Ileak), which was attributed to the formation of nanoelectropores or larger pores in the plasma membrane. We demonstrate that the increase in Ileak, in combination with a residual series resistance, leads to an error in the holding potential in the patch clamp experiments and an unanticipated inactivation of the sodium channels. We conclude that the observed inhibition of INa may be largely, if not fully, artifactual. Bioelectromagnetics 34:162–164, 2013. © 2012 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bem.21754 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1273425561</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2862313311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3914-7994f77df265809babbf99f58ad4d9ba151bc5d4e798168faacaa6a00a2f8983</originalsourceid><addsrcrecordid>eNp1kM1O3DAUha2qVRmmXfQFkKVuYBGwnTi2V4ii4Uea0qqg0p3l-EcYEntqJ6Lz9vUwwKJSV76WvvPp3gPAJ4wOMULkqLPDIcGMNm_ADFOCK8zbX2_BDGHBKt60ZAfs5nyPEOIc1e_BDiGixTWtZ-D2Oho_DVBPKdkwQh_ufOdHHwPs1jCoELPVMRi4mvpsDbS91WPyGjpvewP3Q_6-ODuonNIjjAmqNPrNfPwBvHOqJD4-v3Nwc7a4Ob2olt_OL09PlpWuBW4qJkTjGDOOtJQj0amuc0I4ypVpTPliijtNTWOZ4LjlTimtVKsQUsRxwes52N9qVyn-nmwe5eCztn2vgo1TlpiwuiGUlmvn4PM_6H2cUijLFarllJZCNsKDLaVTzDlZJ1fJDyqtJUZyU7YsZcunsgu792ycusGaV_Kl3QIcbYFH39v1_03yy-Lri7LaJnwe7Z_XhEoPsmU1o_L26lwuf_CL6yu-lD_rv4bjl0U</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1268551358</pqid></control><display><type>article</type><title>Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact?</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Verkerk, Arie O. ; van Ginneken, Antoni C.G. ; Wilders, Ronald</creator><creatorcontrib>Verkerk, Arie O. ; van Ginneken, Antoni C.G. ; Wilders, Ronald</creatorcontrib><description>In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage‐gated sodium current (INa) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non‐inactivating “leak” current (Ileak), which was attributed to the formation of nanoelectropores or larger pores in the plasma membrane. We demonstrate that the increase in Ileak, in combination with a residual series resistance, leads to an error in the holding potential in the patch clamp experiments and an unanticipated inactivation of the sodium channels. We conclude that the observed inhibition of INa may be largely, if not fully, artifactual. Bioelectromagnetics 34:162–164, 2013. © 2012 Wiley Periodicals, Inc.</description><identifier>ISSN: 0197-8462</identifier><identifier>EISSN: 1521-186X</identifier><identifier>DOI: 10.1002/bem.21754</identifier><identifier>PMID: 22961353</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Calcium Channels - metabolism ; Calcium Signaling ; Electric Conductivity ; inactivation ; patch clamp ; series resistance ; Sodium - metabolism ; Sodium Channels - metabolism ; sodium current ; voltage clamp</subject><ispartof>Bioelectromagnetics, 2013-02, Vol.34 (2), p.162-164</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3914-7994f77df265809babbf99f58ad4d9ba151bc5d4e798168faacaa6a00a2f8983</citedby><cites>FETCH-LOGICAL-c3914-7994f77df265809babbf99f58ad4d9ba151bc5d4e798168faacaa6a00a2f8983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbem.21754$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbem.21754$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22961353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verkerk, Arie O.</creatorcontrib><creatorcontrib>van Ginneken, Antoni C.G.</creatorcontrib><creatorcontrib>Wilders, Ronald</creatorcontrib><title>Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact?</title><title>Bioelectromagnetics</title><addtitle>Bioelectromagnetics</addtitle><description>In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage‐gated sodium current (INa) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non‐inactivating “leak” current (Ileak), which was attributed to the formation of nanoelectropores or larger pores in the plasma membrane. We demonstrate that the increase in Ileak, in combination with a residual series resistance, leads to an error in the holding potential in the patch clamp experiments and an unanticipated inactivation of the sodium channels. We conclude that the observed inhibition of INa may be largely, if not fully, artifactual. Bioelectromagnetics 34:162–164, 2013. © 2012 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Signaling</subject><subject>Electric Conductivity</subject><subject>inactivation</subject><subject>patch clamp</subject><subject>series resistance</subject><subject>Sodium - metabolism</subject><subject>Sodium Channels - metabolism</subject><subject>sodium current</subject><subject>voltage clamp</subject><issn>0197-8462</issn><issn>1521-186X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1O3DAUha2qVRmmXfQFkKVuYBGwnTi2V4ii4Uea0qqg0p3l-EcYEntqJ6Lz9vUwwKJSV76WvvPp3gPAJ4wOMULkqLPDIcGMNm_ADFOCK8zbX2_BDGHBKt60ZAfs5nyPEOIc1e_BDiGixTWtZ-D2Oho_DVBPKdkwQh_ufOdHHwPs1jCoELPVMRi4mvpsDbS91WPyGjpvewP3Q_6-ODuonNIjjAmqNPrNfPwBvHOqJD4-v3Nwc7a4Ob2olt_OL09PlpWuBW4qJkTjGDOOtJQj0amuc0I4ypVpTPliijtNTWOZ4LjlTimtVKsQUsRxwes52N9qVyn-nmwe5eCztn2vgo1TlpiwuiGUlmvn4PM_6H2cUijLFarllJZCNsKDLaVTzDlZJ1fJDyqtJUZyU7YsZcunsgu792ycusGaV_Kl3QIcbYFH39v1_03yy-Lri7LaJnwe7Z_XhEoPsmU1o_L26lwuf_CL6yu-lD_rv4bjl0U</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Verkerk, Arie O.</creator><creator>van Ginneken, Antoni C.G.</creator><creator>Wilders, Ronald</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201302</creationdate><title>Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact?</title><author>Verkerk, Arie O. ; van Ginneken, Antoni C.G. ; Wilders, Ronald</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3914-7994f77df265809babbf99f58ad4d9ba151bc5d4e798168faacaa6a00a2f8983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Signaling</topic><topic>Electric Conductivity</topic><topic>inactivation</topic><topic>patch clamp</topic><topic>series resistance</topic><topic>Sodium - metabolism</topic><topic>Sodium Channels - metabolism</topic><topic>sodium current</topic><topic>voltage clamp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verkerk, Arie O.</creatorcontrib><creatorcontrib>van Ginneken, Antoni C.G.</creatorcontrib><creatorcontrib>Wilders, Ronald</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioelectromagnetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verkerk, Arie O.</au><au>van Ginneken, Antoni C.G.</au><au>Wilders, Ronald</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact?</atitle><jtitle>Bioelectromagnetics</jtitle><addtitle>Bioelectromagnetics</addtitle><date>2013-02</date><risdate>2013</risdate><volume>34</volume><issue>2</issue><spage>162</spage><epage>164</epage><pages>162-164</pages><issn>0197-8462</issn><eissn>1521-186X</eissn><abstract>In two recent publications in Bioelectromagnetics it has been demonstrated that the voltage‐gated sodium current (INa) is inhibited in response to a nanosecond pulsed electric field (nsPEF). At the same time, there was an increase in a non‐inactivating “leak” current (Ileak), which was attributed to the formation of nanoelectropores or larger pores in the plasma membrane. We demonstrate that the increase in Ileak, in combination with a residual series resistance, leads to an error in the holding potential in the patch clamp experiments and an unanticipated inactivation of the sodium channels. We conclude that the observed inhibition of INa may be largely, if not fully, artifactual. Bioelectromagnetics 34:162–164, 2013. © 2012 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22961353</pmid><doi>10.1002/bem.21754</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0197-8462 |
| ispartof | Bioelectromagnetics, 2013-02, Vol.34 (2), p.162-164 |
| issn | 0197-8462 1521-186X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1273425561 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Calcium Channels - metabolism Calcium Signaling Electric Conductivity inactivation patch clamp series resistance Sodium - metabolism Sodium Channels - metabolism sodium current voltage clamp |
| title | Sodium current inhibition by nanosecond pulsed electric field (nsPEF)-fact or artifact? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T18%3A32%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sodium%20current%20inhibition%20by%20nanosecond%20pulsed%20electric%20field%20(nsPEF)-fact%20or%20artifact?&rft.jtitle=Bioelectromagnetics&rft.au=Verkerk,%20Arie%20O.&rft.date=2013-02&rft.volume=34&rft.issue=2&rft.spage=162&rft.epage=164&rft.pages=162-164&rft.issn=0197-8462&rft.eissn=1521-186X&rft_id=info:doi/10.1002/bem.21754&rft_dat=%3Cproquest_cross%3E2862313311%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1268551358&rft_id=info:pmid/22961353&rfr_iscdi=true |