The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity
Background/purpose The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test. Methods and materials The...
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| Veröffentlicht in: | Skin research and technology 2013-02, Vol.19 (1), p.e200-e208 |
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| creator | Kobiela, Tomasz Lelen-Kaminska, Karolina Stepulak, Michal Lekka, Malgorzata Malejczyk, Magdalena Arct, Jacek Majewski, Slawomir |
| description | Background/purpose
The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test.
Methods and materials
The effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively.
Results
The increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS‐treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells.
Conclusion
These results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs. |
| doi_str_mv | 10.1111/j.1600-0846.2012.00628.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_proquest_miscellaneous_1273316477</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2862100091</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4348-2253fd9d1507d3e83f42b3946721de70dbaa860a99a495ac606de21740b1d83b3</originalsourceid><addsrcrecordid>eNqNkUtv1DAURi0EokPhLyBLbNgk-JH4IbFBLRTUEahlULvCcuIb1dNMUmwHJvx6PJ0yC1Z4Y1s-5_rquwhhSkqa15t1SQUhBVGVKBmhrCREMFVuH6HF4eExWhBNdCFrdn2EnsW4JoTUmvKn6IgxIalWYoG-r24A-6HrJxhawGOH4xQ62yY7pIjt4PDN7MLYz7_B4bswJvBDxOOAbyHY5IexnRNE_NPbxvc-zfcK9DYm3-brc_Sks32EFw_7Mfr24f3q5GOx_HL26eTdsmgrXqmCsZp3TjtaE-k4KN5VrOG6EpJRB5K4xloliNXaVrq2rSDCAaOyIg11ijf8GL3e180t_pggJrPxsYW-twOMUzSUSc6pqKTM6Kt_0PU4hSF3lymhqK6oIplSe6oNY4wBOnMX_MaG2VBidjMwa7OL2uyiNrsZmPsZmG1WXz58MDUbcAfxb-gZeLsHfvke5v8ubL5ervIh68Ve9zHB9qDbcGuE5LI2V5_PzMX1-fL88uLKnPI_FAqkrw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1268194180</pqid></control><display><type>article</type><title>The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity</title><source>Wiley-Blackwell Open Access Collection</source><creator>Kobiela, Tomasz ; Lelen-Kaminska, Karolina ; Stepulak, Michal ; Lekka, Malgorzata ; Malejczyk, Magdalena ; Arct, Jacek ; Majewski, Slawomir</creator><creatorcontrib>Kobiela, Tomasz ; Lelen-Kaminska, Karolina ; Stepulak, Michal ; Lekka, Malgorzata ; Malejczyk, Magdalena ; Arct, Jacek ; Majewski, Slawomir</creatorcontrib><description>Background/purpose
The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test.
Methods and materials
The effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively.
Results
The increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS‐treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells.
Conclusion
These results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs.</description><identifier>ISSN: 0909-752X</identifier><identifier>EISSN: 1600-0846</identifier><identifier>DOI: 10.1111/j.1600-0846.2012.00628.x</identifier><identifier>PMID: 22671986</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Actin Cytoskeleton - drug effects ; Actin Cytoskeleton - physiology ; Antigens, Surface - drug effects ; Antigens, Surface - physiology ; atomic force microscopy ; Cell Line ; Cell Membrane - drug effects ; Cell Membrane - physiology ; Collagen - toxicity ; cytoskeleton ; Cytoskeleton - drug effects ; Cytoskeleton - physiology ; Elasticity - drug effects ; Elasticity - physiology ; Humans ; Hydrolysis ; hydrolyzed collagen ; keratinocyte stiffness ; Keratinocytes - cytology ; Keratinocytes - drug effects ; Keratinocytes - physiology ; Microscopy, Atomic Force ; Models, Biological ; Sodium Dodecyl Sulfate - toxicity ; sodium lauryl sulphate ; Surface-Active Agents - toxicity</subject><ispartof>Skin research and technology, 2013-02, Vol.19 (1), p.e200-e208</ispartof><rights>2012 John Wiley & Sons A/S</rights><rights>2012 John Wiley & Sons A/S.</rights><rights>Copyright © 2013 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4348-2253fd9d1507d3e83f42b3946721de70dbaa860a99a495ac606de21740b1d83b3</citedby><cites>FETCH-LOGICAL-c4348-2253fd9d1507d3e83f42b3946721de70dbaa860a99a495ac606de21740b1d83b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0846.2012.00628.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0846.2012.00628.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,11561,27923,27924,45573,45574,46051,46475</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1600-0846.2012.00628.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22671986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobiela, Tomasz</creatorcontrib><creatorcontrib>Lelen-Kaminska, Karolina</creatorcontrib><creatorcontrib>Stepulak, Michal</creatorcontrib><creatorcontrib>Lekka, Malgorzata</creatorcontrib><creatorcontrib>Malejczyk, Magdalena</creatorcontrib><creatorcontrib>Arct, Jacek</creatorcontrib><creatorcontrib>Majewski, Slawomir</creatorcontrib><title>The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity</title><title>Skin research and technology</title><addtitle>Skin Res Technol</addtitle><description>Background/purpose
The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test.
Methods and materials
The effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively.
Results
The increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS‐treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells.
Conclusion
These results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs.</description><subject>Actin Cytoskeleton - drug effects</subject><subject>Actin Cytoskeleton - physiology</subject><subject>Antigens, Surface - drug effects</subject><subject>Antigens, Surface - physiology</subject><subject>atomic force microscopy</subject><subject>Cell Line</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - physiology</subject><subject>Collagen - toxicity</subject><subject>cytoskeleton</subject><subject>Cytoskeleton - drug effects</subject><subject>Cytoskeleton - physiology</subject><subject>Elasticity - drug effects</subject><subject>Elasticity - physiology</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>hydrolyzed collagen</subject><subject>keratinocyte stiffness</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - physiology</subject><subject>Microscopy, Atomic Force</subject><subject>Models, Biological</subject><subject>Sodium Dodecyl Sulfate - toxicity</subject><subject>sodium lauryl sulphate</subject><subject>Surface-Active Agents - toxicity</subject><issn>0909-752X</issn><issn>1600-0846</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAURi0EokPhLyBLbNgk-JH4IbFBLRTUEahlULvCcuIb1dNMUmwHJvx6PJ0yC1Z4Y1s-5_rquwhhSkqa15t1SQUhBVGVKBmhrCREMFVuH6HF4eExWhBNdCFrdn2EnsW4JoTUmvKn6IgxIalWYoG-r24A-6HrJxhawGOH4xQ62yY7pIjt4PDN7MLYz7_B4bswJvBDxOOAbyHY5IexnRNE_NPbxvc-zfcK9DYm3-brc_Sks32EFw_7Mfr24f3q5GOx_HL26eTdsmgrXqmCsZp3TjtaE-k4KN5VrOG6EpJRB5K4xloliNXaVrq2rSDCAaOyIg11ijf8GL3e180t_pggJrPxsYW-twOMUzSUSc6pqKTM6Kt_0PU4hSF3lymhqK6oIplSe6oNY4wBOnMX_MaG2VBidjMwa7OL2uyiNrsZmPsZmG1WXz58MDUbcAfxb-gZeLsHfvke5v8ubL5ervIh68Ve9zHB9qDbcGuE5LI2V5_PzMX1-fL88uLKnPI_FAqkrw</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Kobiela, Tomasz</creator><creator>Lelen-Kaminska, Karolina</creator><creator>Stepulak, Michal</creator><creator>Lekka, Malgorzata</creator><creator>Malejczyk, Magdalena</creator><creator>Arct, Jacek</creator><creator>Majewski, Slawomir</creator><general>Blackwell Publishing Ltd</general><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201302</creationdate><title>The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity</title><author>Kobiela, Tomasz ; Lelen-Kaminska, Karolina ; Stepulak, Michal ; Lekka, Malgorzata ; Malejczyk, Magdalena ; Arct, Jacek ; Majewski, Slawomir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4348-2253fd9d1507d3e83f42b3946721de70dbaa860a99a495ac606de21740b1d83b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actin Cytoskeleton - drug effects</topic><topic>Actin Cytoskeleton - physiology</topic><topic>Antigens, Surface - drug effects</topic><topic>Antigens, Surface - physiology</topic><topic>atomic force microscopy</topic><topic>Cell Line</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - physiology</topic><topic>Collagen - toxicity</topic><topic>cytoskeleton</topic><topic>Cytoskeleton - drug effects</topic><topic>Cytoskeleton - physiology</topic><topic>Elasticity - drug effects</topic><topic>Elasticity - physiology</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>hydrolyzed collagen</topic><topic>keratinocyte stiffness</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - physiology</topic><topic>Microscopy, Atomic Force</topic><topic>Models, Biological</topic><topic>Sodium Dodecyl Sulfate - toxicity</topic><topic>sodium lauryl sulphate</topic><topic>Surface-Active Agents - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobiela, Tomasz</creatorcontrib><creatorcontrib>Lelen-Kaminska, Karolina</creatorcontrib><creatorcontrib>Stepulak, Michal</creatorcontrib><creatorcontrib>Lekka, Malgorzata</creatorcontrib><creatorcontrib>Malejczyk, Magdalena</creatorcontrib><creatorcontrib>Arct, Jacek</creatorcontrib><creatorcontrib>Majewski, Slawomir</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Skin research and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Kobiela, Tomasz</au><au>Lelen-Kaminska, Karolina</au><au>Stepulak, Michal</au><au>Lekka, Malgorzata</au><au>Malejczyk, Magdalena</au><au>Arct, Jacek</au><au>Majewski, Slawomir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity</atitle><jtitle>Skin research and technology</jtitle><addtitle>Skin Res Technol</addtitle><date>2013-02</date><risdate>2013</risdate><volume>19</volume><issue>1</issue><spage>e200</spage><epage>e208</epage><pages>e200-e208</pages><issn>0909-752X</issn><eissn>1600-0846</eissn><abstract>Background/purpose
The knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test.
Methods and materials
The effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively.
Results
The increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS‐treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells.
Conclusion
These results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>22671986</pmid><doi>10.1111/j.1600-0846.2012.00628.x</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0909-752X |
| ispartof | Skin research and technology, 2013-02, Vol.19 (1), p.e200-e208 |
| issn | 0909-752X 1600-0846 |
| language | eng |
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| source | Wiley-Blackwell Open Access Collection |
| subjects | Actin Cytoskeleton - drug effects Actin Cytoskeleton - physiology Antigens, Surface - drug effects Antigens, Surface - physiology atomic force microscopy Cell Line Cell Membrane - drug effects Cell Membrane - physiology Collagen - toxicity cytoskeleton Cytoskeleton - drug effects Cytoskeleton - physiology Elasticity - drug effects Elasticity - physiology Humans Hydrolysis hydrolyzed collagen keratinocyte stiffness Keratinocytes - cytology Keratinocytes - drug effects Keratinocytes - physiology Microscopy, Atomic Force Models, Biological Sodium Dodecyl Sulfate - toxicity sodium lauryl sulphate Surface-Active Agents - toxicity |
| title | The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity |
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