Enzyme-cleavable tandem peptides for quantitative studies in MS-based proteomics

A novel type of peptide standard is introduced that consists of two peptides combined in one synthetic molecule and separated by a proteolytic cleavage site. Upon enzymatic digestion, the two peptides are released in a molar one‐to‐one ratio. This method enables the generation of exact equimolar mix...

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Veröffentlicht in:	Proteomics (Weinheim) 2012-12, Vol.12 (23-24), p.3470-3474
Hauptverfasser:	Winter, Dominic, Hung, Chien-Wen, Jaskolla, Thorsten W., Karas, Michael, Lehmann, Wolf D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence ESI Ionization efficiency Ions MALDI Molecular Sequence Data Peptide quantification Peptides Peptides - chemistry Peptides - metabolism Proteins Proteolytic cleavage Proteomics Proteomics - methods Spectrometry, Mass, Electrospray Ionization - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Technology Trypsin - metabolism
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