Development and validation of a novel Taqman-based real-time RT-PCR assay suitable for demonstrating freedom from viral haemorrhagic septicaemia virus

Viral haemorrhagic septicaemia (VHS) is a serious disease in several fish species. VHS is caused by the rhabdovirus viral haemorrhagic septicaemia virus (VHSV). To prevent spreading of the pathogen, it is important to use a fast, robust, sensitive and specific diagnostic tool to identify the infecte...

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Veröffentlicht in:	Journal of fish diseases 2013-01, Vol.36 (1), p.9-23
Hauptverfasser:	Jonstrup, S P, Kahns, S, Skall, H F, Boutrup, T S, Olesen, N J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals DNA Primers Enzyme-Linked Immunosorbent Assay - veterinary Fish Diseases - diagnosis Fisheries - methods Novirhabdovirus - genetics Real-Time Polymerase Chain Reaction - standards Real-Time Polymerase Chain Reaction - veterinary real-time RT-PCR Reproducibility of Results Rhabdoviridae Infections - diagnosis Rhabdoviridae Infections - veterinary Rhabdovirus RNA, Viral - genetics Sensitivity and Specificity validation viral haemorrhagic septicaemia viral haemorrhagic septicaemia virus Viral hemorrhagic septicemia virus
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