CXCR7 mediates SDF1-induced melanocyte migration
Summary Melanoblasts are derived from the neural crest and migrate to the dermal/epidermal border of skin and hair bulges. Although melanoblast migration during embryogenesis has been well investigated, there are only a few reports regarding the migration of mature melanocytes. Here, we demonstrate...
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| Veröffentlicht in: | Pigment cell and melanoma research 2013-01, Vol.26 (1), p.58-66 |
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| container_title | Pigment cell and melanoma research |
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| creator | Lee, Eunkyung Han, Jiyeon Kim, Kwangmi Choi, Hyunjung Cho, Eun-Gyung Lee, Tae Ryong |
| description | Summary
Melanoblasts are derived from the neural crest and migrate to the dermal/epidermal border of skin and hair bulges. Although melanoblast migration during embryogenesis has been well investigated, there are only a few reports regarding the migration of mature melanocytes. Here, we demonstrate that a chemokine, stromal‐derived factor‐1 (SDF1, also known as CXCL12), and one of its receptor CXCR7 regulate normal human epidermal melanocyte (NHEM) migration. We found that SDF1 induces the directional migration of NHEMs. Interestingly, although both CXCR4 and CXCR7 are expressed in NHEMs, blockade of CXCR4 using a CXCR4‐specific neutralizing antibody did not exert any influence on the SDF1‐induced migration of NHEMs, whereas blockade of CXCR7 using a CXCR7‐specific neutralizing antibody did influence migration. Furthermore, SDF1‐induced NHEMs migration exhibited the early hallmark events of CXCR7 signaling associated with MAP kinase activation. It is known that the phosphorylation of ERK through CXCR7 signaling is mediated by β‐arrestins. The treatment of NHEMs with SDF1 resulted in the phosphorylation of ERK in a β‐arrestin 2‐dependent manner. These results suggest that melanocytes may have a unique mechanism of migration via SDF1/CXCR7 signaling that is different from that of other cell types. |
| doi_str_mv | 10.1111/pcmr.12024 |
| format | Article |
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Melanoblasts are derived from the neural crest and migrate to the dermal/epidermal border of skin and hair bulges. Although melanoblast migration during embryogenesis has been well investigated, there are only a few reports regarding the migration of mature melanocytes. Here, we demonstrate that a chemokine, stromal‐derived factor‐1 (SDF1, also known as CXCL12), and one of its receptor CXCR7 regulate normal human epidermal melanocyte (NHEM) migration. We found that SDF1 induces the directional migration of NHEMs. Interestingly, although both CXCR4 and CXCR7 are expressed in NHEMs, blockade of CXCR4 using a CXCR4‐specific neutralizing antibody did not exert any influence on the SDF1‐induced migration of NHEMs, whereas blockade of CXCR7 using a CXCR7‐specific neutralizing antibody did influence migration. Furthermore, SDF1‐induced NHEMs migration exhibited the early hallmark events of CXCR7 signaling associated with MAP kinase activation. It is known that the phosphorylation of ERK through CXCR7 signaling is mediated by β‐arrestins. The treatment of NHEMs with SDF1 resulted in the phosphorylation of ERK in a β‐arrestin 2‐dependent manner. These results suggest that melanocytes may have a unique mechanism of migration via SDF1/CXCR7 signaling that is different from that of other cell types.</description><identifier>ISSN: 1755-1471</identifier><identifier>EISSN: 1755-148X</identifier><identifier>DOI: 10.1111/pcmr.12024</identifier><identifier>PMID: 22978759</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Antibodies, Neutralizing - pharmacology ; Arrestins - metabolism ; beta-Arrestin 2 ; beta-Arrestins ; Cell Movement - drug effects ; Chemokine CXCL12 - pharmacology ; CXCR7 ; Epidermal Cells ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Humans ; MAP Kinase Signaling System - drug effects ; melanocyte ; Melanocytes - cytology ; Melanocytes - drug effects ; Melanocytes - enzymology ; Melanocytes - metabolism ; Migration ; Phosphorylation ; Proteins ; Receptors, CXCR - genetics ; Receptors, CXCR - metabolism ; stromal-derived factor 1 ; β-arrestin2</subject><ispartof>Pigment cell and melanoma research, 2013-01, Vol.26 (1), p.58-66</ispartof><rights>2012 John Wiley & Sons A/S</rights><rights>2012 John Wiley & Sons A/S.</rights><rights>Copyright © 2013 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpcmr.12024$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpcmr.12024$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22978759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Eunkyung</creatorcontrib><creatorcontrib>Han, Jiyeon</creatorcontrib><creatorcontrib>Kim, Kwangmi</creatorcontrib><creatorcontrib>Choi, Hyunjung</creatorcontrib><creatorcontrib>Cho, Eun-Gyung</creatorcontrib><creatorcontrib>Lee, Tae Ryong</creatorcontrib><title>CXCR7 mediates SDF1-induced melanocyte migration</title><title>Pigment cell and melanoma research</title><addtitle>Pigment Cell Melanoma Res</addtitle><description>Summary
Melanoblasts are derived from the neural crest and migrate to the dermal/epidermal border of skin and hair bulges. Although melanoblast migration during embryogenesis has been well investigated, there are only a few reports regarding the migration of mature melanocytes. Here, we demonstrate that a chemokine, stromal‐derived factor‐1 (SDF1, also known as CXCL12), and one of its receptor CXCR7 regulate normal human epidermal melanocyte (NHEM) migration. We found that SDF1 induces the directional migration of NHEMs. Interestingly, although both CXCR4 and CXCR7 are expressed in NHEMs, blockade of CXCR4 using a CXCR4‐specific neutralizing antibody did not exert any influence on the SDF1‐induced migration of NHEMs, whereas blockade of CXCR7 using a CXCR7‐specific neutralizing antibody did influence migration. Furthermore, SDF1‐induced NHEMs migration exhibited the early hallmark events of CXCR7 signaling associated with MAP kinase activation. It is known that the phosphorylation of ERK through CXCR7 signaling is mediated by β‐arrestins. The treatment of NHEMs with SDF1 resulted in the phosphorylation of ERK in a β‐arrestin 2‐dependent manner. These results suggest that melanocytes may have a unique mechanism of migration via SDF1/CXCR7 signaling that is different from that of other cell types.</description><subject>Antibodies, Neutralizing - pharmacology</subject><subject>Arrestins - metabolism</subject><subject>beta-Arrestin 2</subject><subject>beta-Arrestins</subject><subject>Cell Movement - drug effects</subject><subject>Chemokine CXCL12 - pharmacology</subject><subject>CXCR7</subject><subject>Epidermal Cells</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Humans</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>melanocyte</subject><subject>Melanocytes - cytology</subject><subject>Melanocytes - drug effects</subject><subject>Melanocytes - enzymology</subject><subject>Melanocytes - metabolism</subject><subject>Migration</subject><subject>Phosphorylation</subject><subject>Proteins</subject><subject>Receptors, CXCR - genetics</subject><subject>Receptors, CXCR - metabolism</subject><subject>stromal-derived factor 1</subject><subject>β-arrestin2</subject><issn>1755-1471</issn><issn>1755-148X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkMtOwzAQRS0EolDY8AGoEhs2KX7EmXgJgRakUlAB0Z2VOjZyyaPEiaB_j_ugC7zxyHPu-M5F6IzgPvHnaqGKuk8opuEeOiLAeUDCeLq_q4F00LFzc4wjzAU7RB1KBcTAxRHCyTSZQK_QmU0b7XovtwMS2DJrlc78a56WlVo2ulfYjzptbFWeoAOT5k6fbu8uehvcvSb3wehp-JBcjwLLYh4GoGPMIv8bw0JEGQbDqeZspiE2iivBtVKADQMKkcAqw5kJM0KEwcbEDGLWRZebuYu6-mq1a2RhndK5d6Sr1klCgVGgIScevfiHzqu2Lr07TzHBAWPKPXW-pdqZX1cualuk9VL-ZeEBsgG-ba6Xuz7BcpWyXKUs1ynL5-Rxsq68JthorGv0z06T1p8yAgZcvo-HMplGdCTIWN6wX2LGevQ</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Lee, Eunkyung</creator><creator>Han, Jiyeon</creator><creator>Kim, Kwangmi</creator><creator>Choi, Hyunjung</creator><creator>Cho, Eun-Gyung</creator><creator>Lee, Tae Ryong</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201301</creationdate><title>CXCR7 mediates SDF1-induced melanocyte migration</title><author>Lee, Eunkyung ; Han, Jiyeon ; Kim, Kwangmi ; Choi, Hyunjung ; Cho, Eun-Gyung ; Lee, Tae Ryong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3854-7e803659330996d07f52e53be78fc5c95ecc70f3727690cd0df4d119f0ff83783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibodies, Neutralizing - pharmacology</topic><topic>Arrestins - metabolism</topic><topic>beta-Arrestin 2</topic><topic>beta-Arrestins</topic><topic>Cell Movement - drug effects</topic><topic>Chemokine CXCL12 - pharmacology</topic><topic>CXCR7</topic><topic>Epidermal Cells</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Humans</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>melanocyte</topic><topic>Melanocytes - cytology</topic><topic>Melanocytes - drug effects</topic><topic>Melanocytes - enzymology</topic><topic>Melanocytes - metabolism</topic><topic>Migration</topic><topic>Phosphorylation</topic><topic>Proteins</topic><topic>Receptors, CXCR - genetics</topic><topic>Receptors, CXCR - metabolism</topic><topic>stromal-derived factor 1</topic><topic>β-arrestin2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Eunkyung</creatorcontrib><creatorcontrib>Han, Jiyeon</creatorcontrib><creatorcontrib>Kim, Kwangmi</creatorcontrib><creatorcontrib>Choi, Hyunjung</creatorcontrib><creatorcontrib>Cho, Eun-Gyung</creatorcontrib><creatorcontrib>Lee, Tae Ryong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pigment cell and melanoma research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Eunkyung</au><au>Han, Jiyeon</au><au>Kim, Kwangmi</au><au>Choi, Hyunjung</au><au>Cho, Eun-Gyung</au><au>Lee, Tae Ryong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CXCR7 mediates SDF1-induced melanocyte migration</atitle><jtitle>Pigment cell and melanoma research</jtitle><addtitle>Pigment Cell Melanoma Res</addtitle><date>2013-01</date><risdate>2013</risdate><volume>26</volume><issue>1</issue><spage>58</spage><epage>66</epage><pages>58-66</pages><issn>1755-1471</issn><eissn>1755-148X</eissn><abstract>Summary
Melanoblasts are derived from the neural crest and migrate to the dermal/epidermal border of skin and hair bulges. Although melanoblast migration during embryogenesis has been well investigated, there are only a few reports regarding the migration of mature melanocytes. Here, we demonstrate that a chemokine, stromal‐derived factor‐1 (SDF1, also known as CXCL12), and one of its receptor CXCR7 regulate normal human epidermal melanocyte (NHEM) migration. We found that SDF1 induces the directional migration of NHEMs. Interestingly, although both CXCR4 and CXCR7 are expressed in NHEMs, blockade of CXCR4 using a CXCR4‐specific neutralizing antibody did not exert any influence on the SDF1‐induced migration of NHEMs, whereas blockade of CXCR7 using a CXCR7‐specific neutralizing antibody did influence migration. Furthermore, SDF1‐induced NHEMs migration exhibited the early hallmark events of CXCR7 signaling associated with MAP kinase activation. It is known that the phosphorylation of ERK through CXCR7 signaling is mediated by β‐arrestins. The treatment of NHEMs with SDF1 resulted in the phosphorylation of ERK in a β‐arrestin 2‐dependent manner. These results suggest that melanocytes may have a unique mechanism of migration via SDF1/CXCR7 signaling that is different from that of other cell types.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>22978759</pmid><doi>10.1111/pcmr.12024</doi><tpages>9</tpages></addata></record> |
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| subjects | Antibodies, Neutralizing - pharmacology Arrestins - metabolism beta-Arrestin 2 beta-Arrestins Cell Movement - drug effects Chemokine CXCL12 - pharmacology CXCR7 Epidermal Cells Extracellular Signal-Regulated MAP Kinases - metabolism Humans MAP Kinase Signaling System - drug effects melanocyte Melanocytes - cytology Melanocytes - drug effects Melanocytes - enzymology Melanocytes - metabolism Migration Phosphorylation Proteins Receptors, CXCR - genetics Receptors, CXCR - metabolism stromal-derived factor 1 β-arrestin2 |
| title | CXCR7 mediates SDF1-induced melanocyte migration |
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