Proteomic Analysis of Antihypertensive Proteins in Edible Mushrooms
Mushrooms are high in protein content, which makes them potentially a good source of antihypertensive peptides. Among the mushrooms tested, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) had high levels of antihypertensive activity. The protein extr...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2012-12, Vol.60 (50), p.12341-12348 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Lau, Ching-Ching Abdullah, Noorlidah Shuib, Adawiyah Suriza Aminudin, Norhaniza |
| description | Mushrooms are high in protein content, which makes them potentially a good source of antihypertensive peptides. Among the mushrooms tested, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) had high levels of antihypertensive activity. The protein extracts were fractionated by reverse-phase high-performance liquid chromatography (RPHPLC) into six fractions. Fraction 3 from E5Pc (E5PcF3) and fraction 6 from E3Ab (E3AbF6) had the highest antihypertensive activities. SDS-PAGE analysis showed E5PcF3 consisted mainly of low molecular weight proteins, whereas E3AbF6 contained a variety of high to low molecular weight proteins. There were 22 protein clusters detected by SELDI-TOF-MS analysis with five common peaks found in E5PcF3 and E3AbF6, which had m/z values in the range of 3940–11413. This study suggests that the antihypertensive activity in the two mushroom species could be due to proteins with molecular masses ranging from 3 to 10 kDa. |
| doi_str_mv | 10.1021/jf3042159 |
| format | Article |
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Among the mushrooms tested, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) had high levels of antihypertensive activity. The protein extracts were fractionated by reverse-phase high-performance liquid chromatography (RPHPLC) into six fractions. Fraction 3 from E5Pc (E5PcF3) and fraction 6 from E3Ab (E3AbF6) had the highest antihypertensive activities. SDS-PAGE analysis showed E5PcF3 consisted mainly of low molecular weight proteins, whereas E3AbF6 contained a variety of high to low molecular weight proteins. There were 22 protein clusters detected by SELDI-TOF-MS analysis with five common peaks found in E5PcF3 and E3AbF6, which had m/z values in the range of 3940–11413. This study suggests that the antihypertensive activity in the two mushroom species could be due to proteins with molecular masses ranging from 3 to 10 kDa.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf3042159</identifier><identifier>PMID: 23190208</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Agaricus - chemistry ; Agaricus bisporus ; Antihypertensive Agents - chemistry ; antihypertensive effect ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Electrophoresis, Polyacrylamide Gel ; Food industries ; Fruit and vegetable industries ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - chemistry ; Fungal Proteins - pharmacology ; high performance liquid chromatography ; Mass Spectrometry - methods ; molecular weight ; mushrooms ; peptides ; Pleurotus ; Pleurotus - chemistry ; polyacrylamide gel electrophoresis ; protein content ; proteins ; Proteomics</subject><ispartof>Journal of agricultural and food chemistry, 2012-12, Vol.60 (50), p.12341-12348</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a435t-d25a12509487b89c0eaccab307bf8739ea64f54c7b8dcfcd849402402ae79aa03</citedby><cites>FETCH-LOGICAL-a435t-d25a12509487b89c0eaccab307bf8739ea64f54c7b8dcfcd849402402ae79aa03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf3042159$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf3042159$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26732461$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23190208$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lau, Ching-Ching</creatorcontrib><creatorcontrib>Abdullah, Noorlidah</creatorcontrib><creatorcontrib>Shuib, Adawiyah Suriza</creatorcontrib><creatorcontrib>Aminudin, Norhaniza</creatorcontrib><title>Proteomic Analysis of Antihypertensive Proteins in Edible Mushrooms</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Mushrooms are high in protein content, which makes them potentially a good source of antihypertensive peptides. Among the mushrooms tested, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) had high levels of antihypertensive activity. The protein extracts were fractionated by reverse-phase high-performance liquid chromatography (RPHPLC) into six fractions. Fraction 3 from E5Pc (E5PcF3) and fraction 6 from E3Ab (E3AbF6) had the highest antihypertensive activities. SDS-PAGE analysis showed E5PcF3 consisted mainly of low molecular weight proteins, whereas E3AbF6 contained a variety of high to low molecular weight proteins. There were 22 protein clusters detected by SELDI-TOF-MS analysis with five common peaks found in E5PcF3 and E3AbF6, which had m/z values in the range of 3940–11413. This study suggests that the antihypertensive activity in the two mushroom species could be due to proteins with molecular masses ranging from 3 to 10 kDa.</description><subject>Agaricus - chemistry</subject><subject>Agaricus bisporus</subject><subject>Antihypertensive Agents - chemistry</subject><subject>antihypertensive effect</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Food industries</subject><subject>Fruit and vegetable industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - chemistry</subject><subject>Fungal Proteins - pharmacology</subject><subject>high performance liquid chromatography</subject><subject>Mass Spectrometry - methods</subject><subject>molecular weight</subject><subject>mushrooms</subject><subject>peptides</subject><subject>Pleurotus</subject><subject>Pleurotus - chemistry</subject><subject>polyacrylamide gel electrophoresis</subject><subject>protein content</subject><subject>proteins</subject><subject>Proteomics</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EFr2zAUB3AxWtas3WFfYPNlsB7cPslSLB9D6NZCSgdrz-ZZllYF20r17EK-_ZQmay8FgQTvx1-PP2NfOFxwEPxy7QqQgqvqA5txJSBXnOsjNoM0zLWa8xP2iWgNAFqV8JGdiIJXIEDP2PJ3DKMNvTfZYsBuS56y4NJ79I_bjY2jHcg_2-yF-YEyP2RXrW86m91O9BhD6OmMHTvsyH4-3Kfs4efV_fI6X939ulkuVjnKQo15KxRyoaCSumx0ZcCiMdgUUDZOl0VlcS6dkiYNW-NMq2UlQaSDtqwQoThlP_a5mxieJktj3XsytutwsGGimouyECXXxY6e76mJgShaV2-i7zFuaw71rrP6tbNkvx5ip6a37av8X1IC3w8AyWDnIg7G05ubp2_lnCf3be8chhr_xmQe_gjgEoALkFX5loSG6nWYYqqc3lnpH0Mgh70</recordid><startdate>20121219</startdate><enddate>20121219</enddate><creator>Lau, Ching-Ching</creator><creator>Abdullah, Noorlidah</creator><creator>Shuib, Adawiyah Suriza</creator><creator>Aminudin, Norhaniza</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121219</creationdate><title>Proteomic Analysis of Antihypertensive Proteins in Edible Mushrooms</title><author>Lau, Ching-Ching ; Abdullah, Noorlidah ; Shuib, Adawiyah Suriza ; Aminudin, Norhaniza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a435t-d25a12509487b89c0eaccab307bf8739ea64f54c7b8dcfcd849402402ae79aa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agaricus - chemistry</topic><topic>Agaricus bisporus</topic><topic>Antihypertensive Agents - chemistry</topic><topic>antihypertensive effect</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Food industries</topic><topic>Fruit and vegetable industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - chemistry</topic><topic>Fungal Proteins - pharmacology</topic><topic>high performance liquid chromatography</topic><topic>Mass Spectrometry - methods</topic><topic>molecular weight</topic><topic>mushrooms</topic><topic>peptides</topic><topic>Pleurotus</topic><topic>Pleurotus - chemistry</topic><topic>polyacrylamide gel electrophoresis</topic><topic>protein content</topic><topic>proteins</topic><topic>Proteomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lau, Ching-Ching</creatorcontrib><creatorcontrib>Abdullah, Noorlidah</creatorcontrib><creatorcontrib>Shuib, Adawiyah Suriza</creatorcontrib><creatorcontrib>Aminudin, Norhaniza</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lau, Ching-Ching</au><au>Abdullah, Noorlidah</au><au>Shuib, Adawiyah Suriza</au><au>Aminudin, Norhaniza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic Analysis of Antihypertensive Proteins in Edible Mushrooms</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2012-12-19</date><risdate>2012</risdate><volume>60</volume><issue>50</issue><spage>12341</spage><epage>12348</epage><pages>12341-12348</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Mushrooms are high in protein content, which makes them potentially a good source of antihypertensive peptides. Among the mushrooms tested, protein extracts from Pleurotus cystidiosus (E1Pc and E5Pc) and Agaricus bisporus (E1Ab and E3Ab) had high levels of antihypertensive activity. The protein extracts were fractionated by reverse-phase high-performance liquid chromatography (RPHPLC) into six fractions. Fraction 3 from E5Pc (E5PcF3) and fraction 6 from E3Ab (E3AbF6) had the highest antihypertensive activities. SDS-PAGE analysis showed E5PcF3 consisted mainly of low molecular weight proteins, whereas E3AbF6 contained a variety of high to low molecular weight proteins. There were 22 protein clusters detected by SELDI-TOF-MS analysis with five common peaks found in E5PcF3 and E3AbF6, which had m/z values in the range of 3940–11413. This study suggests that the antihypertensive activity in the two mushroom species could be due to proteins with molecular masses ranging from 3 to 10 kDa.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23190208</pmid><doi>10.1021/jf3042159</doi><tpages>8</tpages></addata></record> |
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| subjects | Agaricus - chemistry Agaricus bisporus Antihypertensive Agents - chemistry antihypertensive effect Biological and medical sciences Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - pharmacology high performance liquid chromatography Mass Spectrometry - methods molecular weight mushrooms peptides Pleurotus Pleurotus - chemistry polyacrylamide gel electrophoresis protein content proteins Proteomics |
| title | Proteomic Analysis of Antihypertensive Proteins in Edible Mushrooms |
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