Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70
The genome from the Saccharomyces pastorianus industrial lager brewing strain Weihenstephan 34/70, a natural Saccharomyces cerevisiae/Saccharomyces eubayanus hybrid, indicated the presence of two different maltotriose transporter genes: a new gene in the S. eubayanus subgenome with 81% of homology t...
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| Veröffentlicht in: | Letters in applied microbiology 2013-01, Vol.56 (1), p.21-29 |
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| creator | Cousseau, F.E.M. Alves, S.L. Trichez, D. Stambuk, B.U. |
| description | The genome from the Saccharomyces pastorianus industrial lager brewing strain Weihenstephan 34/70, a natural Saccharomyces cerevisiae/Saccharomyces eubayanus hybrid, indicated the presence of two different maltotriose transporter genes: a new gene in the S. eubayanus subgenome with 81% of homology to the AGT1 permease from S. cerevisiae, and an amplification of the S. eubayanus MTY1 maltotriose permease previously identified in S. pastorianus yeasts. To characterize these S. eubayanus transporter genes, we used a S. cerevisiae strain deleted in the AGT1 permease and introduced the desired permease gene(s) into this locus through homologous recombination. Our results indicate that both the MTY1 and AGT1 genes from the S. eubayanus subgenome encode functional maltotriose transporters that allow fermentation of this sugar by yeast cells, despite their apparent differences in the kinetics of maltotriose‐H+ symport activity. The presence of two maltotriose transporters in the S. eubayanus subgenome not only highlights the importance of sugar transport for efficient maltotriose utilization by industrial yeasts, but these new genes can be used in breeding and/or selection programs aimed at increasing yeast fitness for the efficient fermentation of brewer's wort.
Significance and Impact of the Study:The Saccharomyces pastorianus lager yeast usually ferments maltose and maltotriose efficiently, and transport of these α‐glucosides into the brewer's yeast cell is thought to be rate limiting in the fermentation of these sugars from wort. Our results revealed a new maltotriose transporter gene in the Saccharomyces eubayanus subgenome of the industrial lager brewing strain Weihenstephan 34/70 that allows efficient maltotriose fermentation by yeast cells. The characterization of maltotriose transporters from industrial yeasts opens new opportunities to increase yeast fitness for fermentation of brewer's wort, preventing incomplete or sluggish maltotriose fermentations. |
| doi_str_mv | 10.1111/lam.12011 |
| format | Article |
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Significance and Impact of the Study:The Saccharomyces pastorianus lager yeast usually ferments maltose and maltotriose efficiently, and transport of these α‐glucosides into the brewer's yeast cell is thought to be rate limiting in the fermentation of these sugars from wort. Our results revealed a new maltotriose transporter gene in the Saccharomyces eubayanus subgenome of the industrial lager brewing strain Weihenstephan 34/70 that allows efficient maltotriose fermentation by yeast cells. The characterization of maltotriose transporters from industrial yeasts opens new opportunities to increase yeast fitness for fermentation of brewer's wort, preventing incomplete or sluggish maltotriose fermentations.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/lam.12011</identifier><identifier>PMID: 23061413</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Beer - microbiology ; Biological and medical sciences ; Biological Transport ; brewery ; Carbohydrate Metabolism ; Fermentation ; fermentation biotechnology ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; genes ; Genes, Fungal ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Microbiology ; Phylogeny ; Saccharomyces - genetics ; Saccharomyces - metabolism ; Saccharomyces cerevisiae ; Saccharomyces pastorianus ; Trisaccharides - metabolism ; yeasts</subject><ispartof>Letters in applied microbiology, 2013-01, Vol.56 (1), p.21-29</ispartof><rights>2012 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>2012 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4161-75d90a8379da146569f764d3c986a981bd479e59d5637545840658f58376ae5f3</citedby><cites>FETCH-LOGICAL-c4161-75d90a8379da146569f764d3c986a981bd479e59d5637545840658f58376ae5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Flam.12011$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Flam.12011$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27902,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26731646$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23061413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cousseau, F.E.M.</creatorcontrib><creatorcontrib>Alves, S.L.</creatorcontrib><creatorcontrib>Trichez, D.</creatorcontrib><creatorcontrib>Stambuk, B.U.</creatorcontrib><title>Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>The genome from the Saccharomyces pastorianus industrial lager brewing strain Weihenstephan 34/70, a natural Saccharomyces cerevisiae/Saccharomyces eubayanus hybrid, indicated the presence of two different maltotriose transporter genes: a new gene in the S. eubayanus subgenome with 81% of homology to the AGT1 permease from S. cerevisiae, and an amplification of the S. eubayanus MTY1 maltotriose permease previously identified in S. pastorianus yeasts. To characterize these S. eubayanus transporter genes, we used a S. cerevisiae strain deleted in the AGT1 permease and introduced the desired permease gene(s) into this locus through homologous recombination. Our results indicate that both the MTY1 and AGT1 genes from the S. eubayanus subgenome encode functional maltotriose transporters that allow fermentation of this sugar by yeast cells, despite their apparent differences in the kinetics of maltotriose‐H+ symport activity. The presence of two maltotriose transporters in the S. eubayanus subgenome not only highlights the importance of sugar transport for efficient maltotriose utilization by industrial yeasts, but these new genes can be used in breeding and/or selection programs aimed at increasing yeast fitness for the efficient fermentation of brewer's wort.
Significance and Impact of the Study:The Saccharomyces pastorianus lager yeast usually ferments maltose and maltotriose efficiently, and transport of these α‐glucosides into the brewer's yeast cell is thought to be rate limiting in the fermentation of these sugars from wort. Our results revealed a new maltotriose transporter gene in the Saccharomyces eubayanus subgenome of the industrial lager brewing strain Weihenstephan 34/70 that allows efficient maltotriose fermentation by yeast cells. The characterization of maltotriose transporters from industrial yeasts opens new opportunities to increase yeast fitness for fermentation of brewer's wort, preventing incomplete or sluggish maltotriose fermentations.</description><subject>Beer - microbiology</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>brewery</subject><subject>Carbohydrate Metabolism</subject><subject>Fermentation</subject><subject>fermentation biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Microbiology</subject><subject>Phylogeny</subject><subject>Saccharomyces - genetics</subject><subject>Saccharomyces - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces pastorianus</subject><subject>Trisaccharides - metabolism</subject><subject>yeasts</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0d2K1DAUB_Aiiju7euELSECE9aI7SfPVXC6DXzDihYreldP0dJqlbcakZakP5TOa2RkVFgRzk4v8zjmc_LPsGaNXLJ11D8MVKyhjD7IVE7rItZLfHmYrWiiVl4UUZ9l5jDeU0pIV5nF2VnCqmGB8lf3cdBDAThjcD5icH4lvyQD95KfgfEQyBRjj3ockImmDH8jUIfkE1qZCPywWI8G5hgXGOZI41zsc_YCHNgfYLXVwzT2_hzj54O4qethhIHXAWzfuyILpicQ01I3kK7oOxzjhvoORcLHW9En2qIU-4tPTfZF9efP68-Zdvv349v3meptbwRTLtWwMhZJr0wATSirTaiUabk2pwJSsboQ2KE0jFddSyFJQJctWpgoFKFt-kV0e--6D_z5jnKrBRYt9DyP6OVas0Dx9LtPmf2ihGStLnuiLe_TGz2FMiyTFjZRaMp3Uq6OywccYsK32wQ0QlorR6pB3lfKu7vJO9vmp41wP2PyRvwNO4OUJQLTQtylN6-JfpzRnSqjk1kd363pc_j2x2l5_OI7-BaIpwvw</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Cousseau, F.E.M.</creator><creator>Alves, S.L.</creator><creator>Trichez, D.</creator><creator>Stambuk, B.U.</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201301</creationdate><title>Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70</title><author>Cousseau, F.E.M. ; Alves, S.L. ; Trichez, D. ; Stambuk, B.U.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4161-75d90a8379da146569f764d3c986a981bd479e59d5637545840658f58376ae5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Beer - microbiology</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>brewery</topic><topic>Carbohydrate Metabolism</topic><topic>Fermentation</topic><topic>fermentation biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Microbiology</topic><topic>Phylogeny</topic><topic>Saccharomyces - genetics</topic><topic>Saccharomyces - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces pastorianus</topic><topic>Trisaccharides - metabolism</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cousseau, F.E.M.</creatorcontrib><creatorcontrib>Alves, S.L.</creatorcontrib><creatorcontrib>Trichez, D.</creatorcontrib><creatorcontrib>Stambuk, B.U.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cousseau, F.E.M.</au><au>Alves, S.L.</au><au>Trichez, D.</au><au>Stambuk, B.U.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2013-01</date><risdate>2013</risdate><volume>56</volume><issue>1</issue><spage>21</spage><epage>29</epage><pages>21-29</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>The genome from the Saccharomyces pastorianus industrial lager brewing strain Weihenstephan 34/70, a natural Saccharomyces cerevisiae/Saccharomyces eubayanus hybrid, indicated the presence of two different maltotriose transporter genes: a new gene in the S. eubayanus subgenome with 81% of homology to the AGT1 permease from S. cerevisiae, and an amplification of the S. eubayanus MTY1 maltotriose permease previously identified in S. pastorianus yeasts. To characterize these S. eubayanus transporter genes, we used a S. cerevisiae strain deleted in the AGT1 permease and introduced the desired permease gene(s) into this locus through homologous recombination. Our results indicate that both the MTY1 and AGT1 genes from the S. eubayanus subgenome encode functional maltotriose transporters that allow fermentation of this sugar by yeast cells, despite their apparent differences in the kinetics of maltotriose‐H+ symport activity. The presence of two maltotriose transporters in the S. eubayanus subgenome not only highlights the importance of sugar transport for efficient maltotriose utilization by industrial yeasts, but these new genes can be used in breeding and/or selection programs aimed at increasing yeast fitness for the efficient fermentation of brewer's wort.
Significance and Impact of the Study:The Saccharomyces pastorianus lager yeast usually ferments maltose and maltotriose efficiently, and transport of these α‐glucosides into the brewer's yeast cell is thought to be rate limiting in the fermentation of these sugars from wort. Our results revealed a new maltotriose transporter gene in the Saccharomyces eubayanus subgenome of the industrial lager brewing strain Weihenstephan 34/70 that allows efficient maltotriose fermentation by yeast cells. The characterization of maltotriose transporters from industrial yeasts opens new opportunities to increase yeast fitness for fermentation of brewer's wort, preventing incomplete or sluggish maltotriose fermentations.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23061413</pmid><doi>10.1111/lam.12011</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Beer - microbiology Biological and medical sciences Biological Transport brewery Carbohydrate Metabolism Fermentation fermentation biotechnology Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - metabolism genes Genes, Fungal Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Microbiology Phylogeny Saccharomyces - genetics Saccharomyces - metabolism Saccharomyces cerevisiae Saccharomyces pastorianus Trisaccharides - metabolism yeasts |
| title | Characterization of maltotriose transporters from the Saccharomyces eubayanus subgenome of the hybrid Saccharomyces pastorianus lager brewing yeast strain Weihenstephan 34/70 |
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