Electrochemical characterization of a unique, “neutral” laccase from Flammulina velutipes

The flac1 gene consisted of 1488 bases encodes a novel laccase (Flac1) from Flammulina velutipes. The deduced amino acid sequence of Flac1 with 496 amino acids shows 58–64% homologies with other fungal laccases. The recombinant Flac1 (rFlac1) was heterologously expressed in Pichia pastoris, with sug...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2013-02, Vol.115 (2), p.159-167
Hauptverfasser:	(Otsuka) Saito, Kaori, Kurose, Shinji, Tsujino, Yoshio, Osakai, Toshiyuki, Kataoka, Kunishige, Sakurai, Takeshi, Tamiya, Eiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences amino acids Aminophenols - metabolism Base Sequence Benzothiazoles - metabolism Biological and medical sciences Biotechnology Direct electron transfer Electrochemistry Electrodes electron transfer Electron Transport Flammulina - enzymology Flammulina velutipes Fundamental and applied biological sciences. Psychology fungi genes graphene Heterologous expression Hydrogen-Ion Concentration Laccase Laccase - chemistry Laccase - genetics Laccase - isolation & purification Laccase - metabolism Molecular Sequence Data molecular weight Optimum pH Oxidation-Reduction Oxidoreductases - chemistry Oxidoreductases - metabolism oxygen Phenylenediamines - metabolism Pichia - genetics Pichia - metabolism Pichia pastoris Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Redox potential Substrate Specificity sugars Sulfonic Acids - metabolism
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