Effects of Seminal Fluid Fractions on Plasma and Acrosome Membrane Integrity and Mitochondrial Membrane Potential Determined by Flow Cytometry in Chilled Canine Spermatozoa
Depending on the mammal species, the use of seminal plasma during semen processing for cryopreservation has been found to have both beneficial and detrimental effects. This study was designed to determine the effects of the second (SF) and third [prostatic fluid, (PF)] ejaculate fractions on plasma...
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| Veröffentlicht in: | Reproduction in domestic animals 2012-12, Vol.47 (6), p.1043-1048 |
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| creator | Treulen, F Sánchez, R Risopatrón, J |
| description | Depending on the mammal species, the use of seminal plasma during semen processing for cryopreservation has been found to have both beneficial and detrimental effects. This study was designed to determine the effects of the second (SF) and third [prostatic fluid, (PF)] ejaculate fractions on plasma membrane and acrosome integrity, mitochondrial membrane potential (ΔΨm), phosphatidylserine (PS) translocation and sperm motility in chilled canine spermatozoa by flow cytometry. After pooling the second sperm‐rich fraction of ejaculates from six dogs, samples for each assay were preserved at 5°C for 72 h in egg yolk‐TRIS extender (EYT) alone (control) or supplemented with seminal fluid from the second (EYT‐SF) or third (EYT‐PF) ejaculated fractions. After cold storage, groups EYT‐SF and EYT‐PF showed significantly higher percentages of sperm cells with an intact acrosome [68.8 ± 1.4%, 69.6 ± 2.6% (p |
| doi_str_mv | 10.1111/j.1439-0531.2012.02011.x |
| format | Article |
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This study was designed to determine the effects of the second (SF) and third [prostatic fluid, (PF)] ejaculate fractions on plasma membrane and acrosome integrity, mitochondrial membrane potential (ΔΨm), phosphatidylserine (PS) translocation and sperm motility in chilled canine spermatozoa by flow cytometry. After pooling the second sperm‐rich fraction of ejaculates from six dogs, samples for each assay were preserved at 5°C for 72 h in egg yolk‐TRIS extender (EYT) alone (control) or supplemented with seminal fluid from the second (EYT‐SF) or third (EYT‐PF) ejaculated fractions. After cold storage, groups EYT‐SF and EYT‐PF showed significantly higher percentages of sperm cells with an intact acrosome [68.8 ± 1.4%, 69.6 ± 2.6% (p < 0.01)] and intact plasma membrane [48.1 ± 2.8%, 50.4 ± 8.2% (p < 0.001)] than that observed in EYT [51.7 ± 3.2% and 33.3 ± 4.1% respectively]. Only in EYT‐SF was PS translocation significantly reduced compared to EYT‐PF and EYT [3.9 ± 0.4%, 10.2 ± 2.2% and 9.0 ± 1.5%, respectively (p < 0.001)]. However, significantly diminished sperm motility was observed in EYT‐SF and EYT‐PF compared to EYT [36.8 ± 2.1%, 35.5 ± 2.3% and 78.4 ± 4.7% (p < 0.001)]. No significant differences were detected in ΔΨm (p > 0.05). In conclusion, supplementing semen extenders with seminal fluid from the second or third fractions of the ejaculate supplementation helps to preserve the integrity of the plasma and acrosome membranes along with the mitochondrial membrane potential but seems to compromise the motility of canine spermatozoa chilled for 72 h.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2012.02011.x</identifier><identifier>PMID: 23289122</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>acrosome ; Acrosome - drug effects ; Animal reproduction ; Animals ; Biological and medical sciences ; cold storage ; Cold Temperature ; Cryogenic engineering ; Dogs ; Dogs - physiology ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Male ; Mammalian reproduction. General aspects ; membrane potential ; Membrane Potential, Mitochondrial - drug effects ; Membrane Potential, Mitochondrial - physiology ; Mitochondria ; plasma membrane ; Semen - chemistry ; semen extenders ; Semen Preservation ; seminal plasma ; sperm motility ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2012-12, Vol.47 (6), p.1043-1048</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4891-74b7ade9fa1394e816b93894267a1cb8080a9c0c02562f1979557235168d987b3</citedby><cites>FETCH-LOGICAL-c4891-74b7ade9fa1394e816b93894267a1cb8080a9c0c02562f1979557235168d987b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2012.02011.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2012.02011.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26580746$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23289122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Treulen, F</creatorcontrib><creatorcontrib>Sánchez, R</creatorcontrib><creatorcontrib>Risopatrón, J</creatorcontrib><title>Effects of Seminal Fluid Fractions on Plasma and Acrosome Membrane Integrity and Mitochondrial Membrane Potential Determined by Flow Cytometry in Chilled Canine Spermatozoa</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Depending on the mammal species, the use of seminal plasma during semen processing for cryopreservation has been found to have both beneficial and detrimental effects. This study was designed to determine the effects of the second (SF) and third [prostatic fluid, (PF)] ejaculate fractions on plasma membrane and acrosome integrity, mitochondrial membrane potential (ΔΨm), phosphatidylserine (PS) translocation and sperm motility in chilled canine spermatozoa by flow cytometry. After pooling the second sperm‐rich fraction of ejaculates from six dogs, samples for each assay were preserved at 5°C for 72 h in egg yolk‐TRIS extender (EYT) alone (control) or supplemented with seminal fluid from the second (EYT‐SF) or third (EYT‐PF) ejaculated fractions. After cold storage, groups EYT‐SF and EYT‐PF showed significantly higher percentages of sperm cells with an intact acrosome [68.8 ± 1.4%, 69.6 ± 2.6% (p < 0.01)] and intact plasma membrane [48.1 ± 2.8%, 50.4 ± 8.2% (p < 0.001)] than that observed in EYT [51.7 ± 3.2% and 33.3 ± 4.1% respectively]. Only in EYT‐SF was PS translocation significantly reduced compared to EYT‐PF and EYT [3.9 ± 0.4%, 10.2 ± 2.2% and 9.0 ± 1.5%, respectively (p < 0.001)]. However, significantly diminished sperm motility was observed in EYT‐SF and EYT‐PF compared to EYT [36.8 ± 2.1%, 35.5 ± 2.3% and 78.4 ± 4.7% (p < 0.001)]. No significant differences were detected in ΔΨm (p > 0.05). In conclusion, supplementing semen extenders with seminal fluid from the second or third fractions of the ejaculate supplementation helps to preserve the integrity of the plasma and acrosome membranes along with the mitochondrial membrane potential but seems to compromise the motility of canine spermatozoa chilled for 72 h.</description><subject>acrosome</subject><subject>Acrosome - drug effects</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>cold storage</subject><subject>Cold Temperature</subject><subject>Cryogenic engineering</subject><subject>Dogs</subject><subject>Dogs - physiology</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>membrane potential</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Membrane Potential, Mitochondrial - physiology</subject><subject>Mitochondria</subject><subject>plasma membrane</subject><subject>Semen - chemistry</subject><subject>semen extenders</subject><subject>Semen Preservation</subject><subject>seminal plasma</subject><subject>sperm motility</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNktFu0zAUhiMEYmXwCmAJTeImne0kdnzBRdWuY9JaBt3EpeUkDnNJ4mK7WsMz8ZCcrF2RuCIXiZXz_f-xz-8oQgSPCTzn6zFJExHjLCFjigkdY3iT8e5ZNDoWnkcjLBIWM87yk-iV92uMSZZz_jI6oQnNBaF0FP2-qGtdBo9sjVa6NZ1q0LzZmgrNnSqDsR2UOnTTKN8qpLoKTUpnvW01Wui2cKrT6KoL-rszoX-sL0yw5b3tKmfA6wjd2KC7MPya6aAddNIVKnpoZh_QtA_gGFyPTIem96ZpoDhVHUBotQFaBfvLqtfRi1o1Xr85fE-ju_nF7fRTfP358mo6uY7LFI4V87TgqtKiViQRqc4JK0SSi5QyrkhZ5DjHSpS4xDRjtCaCiyzjNMkIyyuR8yI5jT7sfTfO_txqH2RrfKmbBg5it14SyhOKmaApoO__Qdd262CKQEF3cM4oASrfU8PsvNO13DjTKtdLguWQqFzLITg5BCeHROVjonIH0reHBtui1dVR-BQhAGcHQPlSNTVMuzT-L8eyHPOUAfdxzz2YRvf_vQH5dTYZVqCP93rjg94d9cr9kIwnPJPflpfydjb7sqDLpRz29W7P18pKBffDy7sVOKUY7iGBoSR_ADZo1FI</recordid><startdate>201212</startdate><enddate>201212</enddate><creator>Treulen, F</creator><creator>Sánchez, R</creator><creator>Risopatrón, J</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201212</creationdate><title>Effects of Seminal Fluid Fractions on Plasma and Acrosome Membrane Integrity and Mitochondrial Membrane Potential Determined by Flow Cytometry in Chilled Canine Spermatozoa</title><author>Treulen, F ; Sánchez, R ; Risopatrón, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4891-74b7ade9fa1394e816b93894267a1cb8080a9c0c02562f1979557235168d987b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acrosome</topic><topic>Acrosome - drug effects</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>cold storage</topic><topic>Cold Temperature</topic><topic>Cryogenic engineering</topic><topic>Dogs</topic><topic>Dogs - physiology</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>membrane potential</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Membrane Potential, Mitochondrial - physiology</topic><topic>Mitochondria</topic><topic>plasma membrane</topic><topic>Semen - chemistry</topic><topic>semen extenders</topic><topic>Semen Preservation</topic><topic>seminal plasma</topic><topic>sperm motility</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Treulen, F</creatorcontrib><creatorcontrib>Sánchez, R</creatorcontrib><creatorcontrib>Risopatrón, J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Treulen, F</au><au>Sánchez, R</au><au>Risopatrón, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Seminal Fluid Fractions on Plasma and Acrosome Membrane Integrity and Mitochondrial Membrane Potential Determined by Flow Cytometry in Chilled Canine Spermatozoa</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2012-12</date><risdate>2012</risdate><volume>47</volume><issue>6</issue><spage>1043</spage><epage>1048</epage><pages>1043-1048</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Depending on the mammal species, the use of seminal plasma during semen processing for cryopreservation has been found to have both beneficial and detrimental effects. This study was designed to determine the effects of the second (SF) and third [prostatic fluid, (PF)] ejaculate fractions on plasma membrane and acrosome integrity, mitochondrial membrane potential (ΔΨm), phosphatidylserine (PS) translocation and sperm motility in chilled canine spermatozoa by flow cytometry. After pooling the second sperm‐rich fraction of ejaculates from six dogs, samples for each assay were preserved at 5°C for 72 h in egg yolk‐TRIS extender (EYT) alone (control) or supplemented with seminal fluid from the second (EYT‐SF) or third (EYT‐PF) ejaculated fractions. After cold storage, groups EYT‐SF and EYT‐PF showed significantly higher percentages of sperm cells with an intact acrosome [68.8 ± 1.4%, 69.6 ± 2.6% (p < 0.01)] and intact plasma membrane [48.1 ± 2.8%, 50.4 ± 8.2% (p < 0.001)] than that observed in EYT [51.7 ± 3.2% and 33.3 ± 4.1% respectively]. Only in EYT‐SF was PS translocation significantly reduced compared to EYT‐PF and EYT [3.9 ± 0.4%, 10.2 ± 2.2% and 9.0 ± 1.5%, respectively (p < 0.001)]. However, significantly diminished sperm motility was observed in EYT‐SF and EYT‐PF compared to EYT [36.8 ± 2.1%, 35.5 ± 2.3% and 78.4 ± 4.7% (p < 0.001)]. No significant differences were detected in ΔΨm (p > 0.05). In conclusion, supplementing semen extenders with seminal fluid from the second or third fractions of the ejaculate supplementation helps to preserve the integrity of the plasma and acrosome membranes along with the mitochondrial membrane potential but seems to compromise the motility of canine spermatozoa chilled for 72 h.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23289122</pmid><doi>10.1111/j.1439-0531.2012.02011.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Reproduction in domestic animals, 2012-12, Vol.47 (6), p.1043-1048 |
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| subjects | acrosome Acrosome - drug effects Animal reproduction Animals Biological and medical sciences cold storage Cold Temperature Cryogenic engineering Dogs Dogs - physiology Flow Cytometry Fundamental and applied biological sciences. Psychology Male Mammalian reproduction. General aspects membrane potential Membrane Potential, Mitochondrial - drug effects Membrane Potential, Mitochondrial - physiology Mitochondria plasma membrane Semen - chemistry semen extenders Semen Preservation seminal plasma sperm motility Vertebrates: reproduction |
| title | Effects of Seminal Fluid Fractions on Plasma and Acrosome Membrane Integrity and Mitochondrial Membrane Potential Determined by Flow Cytometry in Chilled Canine Spermatozoa |
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