Thymus mastichina: Chemical Constituents and their Anti-Cancer Activity
The cytotoxicity-guided study of the dichloromethane and ethanol extracts of Thymus mastichna L. using the HCT colon cancer cell line allowed the identification of nine compounds, sakuranetin (1), sterubin (2), oleanolic acid (3), ursolic acid (4), lutein (5), β-sitosterol (6), rosmarinic acid (7),...
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| Veröffentlicht in: | Natural product communications 2012-11, Vol.7 (11), p.1491-1494 |
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| creator | Gordo, Joana Máximo, Patrícia Cabrita, Eurico Lourenço, Ana Oliva, Abel Almeida, Joana Filipe, Mariana Cruz, Pedro Barcia, Rita Santos, Miguel Cruz, Helder |
| description | The cytotoxicity-guided study of the dichloromethane and ethanol extracts of Thymus mastichna L. using the HCT colon cancer cell line allowed the identification of nine compounds, sakuranetin (1), sterubin (2), oleanolic acid (3), ursolic acid (4), lutein (5), β-sitosterol (6), rosmarinic acid (7), 6-hydroxyluteolin-7-O-β-glucopyranoside (8), and 6-hydroxyapigenin-7-O-β-glucopyranoside (9). All compounds were tested for their cytotoxicity against the HCT colon cancer cell line. Compound 4 showed cytotoxicity with GI50 value of 6.8 μg/mL. A fraction composed of a mixture (1:1) of triterpenoid acids 3 and 4 displayed improved cytotoxicity with a GI50 of 2.8 μg/mL suggesting a synergistic behavior. This is the first report on the chemical constituents of Thymus mastichna L. based on structural assignments by spectroscopic analysis. The presence of these constituents identified by colon cancer cytotoxicity-guided activity indicates that extracts of T. mastichna L. may have a protective effect against colon cancers. |
| doi_str_mv | 10.1177/1934578X1200701120 |
| format | Article |
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All compounds were tested for their cytotoxicity against the HCT colon cancer cell line. Compound 4 showed cytotoxicity with GI50 value of 6.8 μg/mL. A fraction composed of a mixture (1:1) of triterpenoid acids 3 and 4 displayed improved cytotoxicity with a GI50 of 2.8 μg/mL suggesting a synergistic behavior. This is the first report on the chemical constituents of Thymus mastichna L. based on structural assignments by spectroscopic analysis. The presence of these constituents identified by colon cancer cytotoxicity-guided activity indicates that extracts of T. mastichna L. may have a protective effect against colon cancers.</description><identifier>ISSN: 1934-578X</identifier><identifier>EISSN: 1555-9475</identifier><identifier>DOI: 10.1177/1934578X1200701120</identifier><identifier>PMID: 23285814</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - isolation & purification ; Carcinoma - drug therapy ; Colorectal Neoplasms - drug therapy ; Drug Screening Assays, Antitumor ; Flavanones - isolation & purification ; HCT116 Cells ; Humans ; Phytotherapy ; Plant Extracts - therapeutic use ; Thymus Plant - chemistry ; Triterpenes - isolation & purification</subject><ispartof>Natural product communications, 2012-11, Vol.7 (11), p.1491-1494</ispartof><rights>2012 SAGE Publications Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-b267a904caeec8e351355b250d9db5dd81e0d2834b72ac000be9d167e9d36f083</citedby><cites>FETCH-LOGICAL-c387t-b267a904caeec8e351355b250d9db5dd81e0d2834b72ac000be9d167e9d36f083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1934578X1200701120$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1934578X1200701120$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,777,781,21947,27834,27905,27906,44926,45314</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/1934578X1200701120?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23285814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gordo, Joana</creatorcontrib><creatorcontrib>Máximo, Patrícia</creatorcontrib><creatorcontrib>Cabrita, Eurico</creatorcontrib><creatorcontrib>Lourenço, Ana</creatorcontrib><creatorcontrib>Oliva, Abel</creatorcontrib><creatorcontrib>Almeida, Joana</creatorcontrib><creatorcontrib>Filipe, Mariana</creatorcontrib><creatorcontrib>Cruz, Pedro</creatorcontrib><creatorcontrib>Barcia, Rita</creatorcontrib><creatorcontrib>Santos, Miguel</creatorcontrib><creatorcontrib>Cruz, Helder</creatorcontrib><title>Thymus mastichina: Chemical Constituents and their Anti-Cancer Activity</title><title>Natural product communications</title><addtitle>Nat Prod Commun</addtitle><description>The cytotoxicity-guided study of the dichloromethane and ethanol extracts of Thymus mastichna L. using the HCT colon cancer cell line allowed the identification of nine compounds, sakuranetin (1), sterubin (2), oleanolic acid (3), ursolic acid (4), lutein (5), β-sitosterol (6), rosmarinic acid (7), 6-hydroxyluteolin-7-O-β-glucopyranoside (8), and 6-hydroxyapigenin-7-O-β-glucopyranoside (9). All compounds were tested for their cytotoxicity against the HCT colon cancer cell line. Compound 4 showed cytotoxicity with GI50 value of 6.8 μg/mL. A fraction composed of a mixture (1:1) of triterpenoid acids 3 and 4 displayed improved cytotoxicity with a GI50 of 2.8 μg/mL suggesting a synergistic behavior. This is the first report on the chemical constituents of Thymus mastichna L. based on structural assignments by spectroscopic analysis. The presence of these constituents identified by colon cancer cytotoxicity-guided activity indicates that extracts of T. mastichna L. may have a protective effect against colon cancers.</description><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - isolation & purification</subject><subject>Carcinoma - drug therapy</subject><subject>Colorectal Neoplasms - drug therapy</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Flavanones - isolation & purification</subject><subject>HCT116 Cells</subject><subject>Humans</subject><subject>Phytotherapy</subject><subject>Plant Extracts - therapeutic use</subject><subject>Thymus Plant - chemistry</subject><subject>Triterpenes - isolation & purification</subject><issn>1934-578X</issn><issn>1555-9475</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoVmr_gAfZo5e1-Wyy3sqiVSh4qeBtySZTN6WbrZus0H9vSqsXwTnMDMMzLzMvQjcE3xMi5ZQUjAup3gnFWGKSyhm6IkKIvOBSnKc-AfmBGKFJCBucQimOeXGJRpRRJRThV2ixavbtELJWh-hM47x-yMoGWmf0Nis7n6ZxAB9Dpr3NYgOuz-Y-urzU3kDqTXRfLu6v0cVabwNMTnWM3p4eV-VzvnxdvJTzZW6YkjGv6UzqAnOjAYwCJggToqYC28LWwlpFAFuqGK8l1SadXENhyUymzGZrrNgY3R11d333OUCIVeuCge1We-iGUBEqGcVcygNKj6jpuxB6WFe73rW631cEVwcPq78epqXbk_5Qt2B_V34cS8D0CAT9AdWmG3qf_v1P8hvsmHj9</recordid><startdate>201211</startdate><enddate>201211</enddate><creator>Gordo, Joana</creator><creator>Máximo, Patrícia</creator><creator>Cabrita, Eurico</creator><creator>Lourenço, Ana</creator><creator>Oliva, Abel</creator><creator>Almeida, Joana</creator><creator>Filipe, Mariana</creator><creator>Cruz, Pedro</creator><creator>Barcia, Rita</creator><creator>Santos, Miguel</creator><creator>Cruz, Helder</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201211</creationdate><title>Thymus mastichina: Chemical Constituents and their Anti-Cancer Activity</title><author>Gordo, Joana ; 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| subjects | Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Carcinoma - drug therapy Colorectal Neoplasms - drug therapy Drug Screening Assays, Antitumor Flavanones - isolation & purification HCT116 Cells Humans Phytotherapy Plant Extracts - therapeutic use Thymus Plant - chemistry Triterpenes - isolation & purification |
| title | Thymus mastichina: Chemical Constituents and their Anti-Cancer Activity |
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