Progesterone (PR), Oestrogen (ER-α and ER-β) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches
Contents The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non‐pregnant females underwent OVH 12 days after...
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| Veröffentlicht in: | Reproduction in domestic animals 2012-12, Vol.47 (s6), p.197-199 |
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| container_title | Reproduction in domestic animals |
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| creator | Derussi, AAP de Souza, RWA Volpato, R Guaitolini, CRF Ackermann, CL Taffarel, MO Cardoso, GS Dal-Pai-Silva, M Lopes, MD |
| description | Contents
The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non‐pregnant females underwent OVH 12 days after the pre‐ovulatory Luteinizant Hormone (LH) surge and served as controls. RT‐qPCR for progesterone (PR), oestrogen (ER‐α and ER‐β) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER‐β expression in the oviduct during early pregnancy was less than in non‐pregnant bitches. In the uterus, the mRNA ER‐β expression was higher in the initial stages of pregnancy. The ER‐α expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (α and β) expression and indirectly on OTR expression in the bitch oviduct and uterus. |
| doi_str_mv | 10.1111/rda.12015 |
| format | Article |
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The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non‐pregnant females underwent OVH 12 days after the pre‐ovulatory Luteinizant Hormone (LH) surge and served as controls. RT‐qPCR for progesterone (PR), oestrogen (ER‐α and ER‐β) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER‐β expression in the oviduct during early pregnancy was less than in non‐pregnant bitches. In the uterus, the mRNA ER‐β expression was higher in the initial stages of pregnancy. The ER‐α expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (α and β) expression and indirectly on OTR expression in the bitch oviduct and uterus.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12015</identifier><identifier>PMID: 23279498</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animal reproduction ; Animals ; Dogs ; Dogs - physiology ; Estrogen Receptor alpha - genetics ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - genetics ; Estrogen Receptor beta - metabolism ; Estrogens ; Female ; Gene expression ; Gene Expression Regulation - physiology ; Oviducts - physiology ; Pregnancy ; Progesterone ; Receptors, Oxytocin - genetics ; Receptors, Oxytocin - metabolism ; Receptors, Progesterone - genetics ; Receptors, Progesterone - metabolism ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Uterus - physiology</subject><ispartof>Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.197-199</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>2012 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4265-d4fe3cabea1b76dff3156fc56d2e3486b8bddcf7491b284c9eef93688851b5973</citedby><cites>FETCH-LOGICAL-c4265-d4fe3cabea1b76dff3156fc56d2e3486b8bddcf7491b284c9eef93688851b5973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.12015$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.12015$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23279498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Derussi, AAP</creatorcontrib><creatorcontrib>de Souza, RWA</creatorcontrib><creatorcontrib>Volpato, R</creatorcontrib><creatorcontrib>Guaitolini, CRF</creatorcontrib><creatorcontrib>Ackermann, CL</creatorcontrib><creatorcontrib>Taffarel, MO</creatorcontrib><creatorcontrib>Cardoso, GS</creatorcontrib><creatorcontrib>Dal-Pai-Silva, M</creatorcontrib><creatorcontrib>Lopes, MD</creatorcontrib><title>Progesterone (PR), Oestrogen (ER-α and ER-β) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non‐pregnant females underwent OVH 12 days after the pre‐ovulatory Luteinizant Hormone (LH) surge and served as controls. RT‐qPCR for progesterone (PR), oestrogen (ER‐α and ER‐β) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER‐β expression in the oviduct during early pregnancy was less than in non‐pregnant bitches. In the uterus, the mRNA ER‐β expression was higher in the initial stages of pregnancy. The ER‐α expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (α and β) expression and indirectly on OTR expression in the bitch oviduct and uterus.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Dogs</subject><subject>Dogs - physiology</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Estrogens</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - physiology</subject><subject>Oviducts - physiology</subject><subject>Pregnancy</subject><subject>Progesterone</subject><subject>Receptors, Oxytocin - genetics</subject><subject>Receptors, Oxytocin - metabolism</subject><subject>Receptors, Progesterone - genetics</subject><subject>Receptors, Progesterone - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Uterus - physiology</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kdFu0zAUhi0EYmVwwQsgS9y0EtliO7Gdy22UgjQ1Jdq0SyuxT7aMNu7sBNq34FXgQXgm3GbdBRK-8fE53__L9o_QWxKfkLBOnSlPCI1J-gyNSMKyKE4ZeY5GccZ4xAWXR-iV9_dxIKQQL9ERZVRkSSZH6OfC2VvwHTjbAh4viskHnIfzrtvi8bSI_vzCZWvwrvo92Zf5ZttZ3YRxflVM8AyCcrpZO_C-sS0Og-4OcP69Mb3u9orr4N97bGu8cHDblu3Qnts2Wh8a502n78C_Ri_qcunhzeN-jK4_Ta8uPkeX-ezLxdllpBPK08gkNTBdVlCSSnBT14ykvNYpNxRYInklK2N0LZKMVFQmOgOow29IKVNSpZlgx2g8-K6dfejDi9Wq8RqWy7IF23tFqAiWKWc79P0_6L3tXRtuF6iEBsskpoGaDJR21nsHtVq7ZlW6rSKx2sWkQkxqH1Ng3z069tUKzBN5yCUApwPwo1nC9v9Oqvh4drCMBkUTwtw8KUr3TXHBRKpu5jN183V-XiwoV5L9BWq6qwA</recordid><startdate>201212</startdate><enddate>201212</enddate><creator>Derussi, AAP</creator><creator>de Souza, RWA</creator><creator>Volpato, R</creator><creator>Guaitolini, CRF</creator><creator>Ackermann, CL</creator><creator>Taffarel, MO</creator><creator>Cardoso, GS</creator><creator>Dal-Pai-Silva, M</creator><creator>Lopes, MD</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201212</creationdate><title>Progesterone (PR), Oestrogen (ER-α and ER-β) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches</title><author>Derussi, AAP ; de Souza, RWA ; Volpato, R ; Guaitolini, CRF ; Ackermann, CL ; Taffarel, MO ; Cardoso, GS ; Dal-Pai-Silva, M ; Lopes, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4265-d4fe3cabea1b76dff3156fc56d2e3486b8bddcf7491b284c9eef93688851b5973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>Dogs</topic><topic>Dogs - physiology</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Estrogens</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - physiology</topic><topic>Oviducts - physiology</topic><topic>Pregnancy</topic><topic>Progesterone</topic><topic>Receptors, Oxytocin - genetics</topic><topic>Receptors, Oxytocin - metabolism</topic><topic>Receptors, Progesterone - genetics</topic><topic>Receptors, Progesterone - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Uterus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Derussi, AAP</creatorcontrib><creatorcontrib>de Souza, RWA</creatorcontrib><creatorcontrib>Volpato, R</creatorcontrib><creatorcontrib>Guaitolini, CRF</creatorcontrib><creatorcontrib>Ackermann, CL</creatorcontrib><creatorcontrib>Taffarel, MO</creatorcontrib><creatorcontrib>Cardoso, GS</creatorcontrib><creatorcontrib>Dal-Pai-Silva, M</creatorcontrib><creatorcontrib>Lopes, MD</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Derussi, AAP</au><au>de Souza, RWA</au><au>Volpato, R</au><au>Guaitolini, CRF</au><au>Ackermann, CL</au><au>Taffarel, MO</au><au>Cardoso, GS</au><au>Dal-Pai-Silva, M</au><au>Lopes, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Progesterone (PR), Oestrogen (ER-α and ER-β) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2012-12</date><risdate>2012</risdate><volume>47</volume><issue>s6</issue><spage>197</spage><epage>199</epage><pages>197-199</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non‐pregnant females underwent OVH 12 days after the pre‐ovulatory Luteinizant Hormone (LH) surge and served as controls. RT‐qPCR for progesterone (PR), oestrogen (ER‐α and ER‐β) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER‐β expression in the oviduct during early pregnancy was less than in non‐pregnant bitches. In the uterus, the mRNA ER‐β expression was higher in the initial stages of pregnancy. The ER‐α expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (α and β) expression and indirectly on OTR expression in the bitch oviduct and uterus.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>23279498</pmid><doi>10.1111/rda.12015</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.197-199 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal reproduction Animals Dogs Dogs - physiology Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Estrogens Female Gene expression Gene Expression Regulation - physiology Oviducts - physiology Pregnancy Progesterone Receptors, Oxytocin - genetics Receptors, Oxytocin - metabolism Receptors, Progesterone - genetics Receptors, Progesterone - metabolism Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism Uterus - physiology |
| title | Progesterone (PR), Oestrogen (ER-α and ER-β) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
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