Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine
8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to ration...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2013-01, Vol.85 (1), p.201-207 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 207 |
|---|---|
| container_issue | 1 |
| container_start_page | 201 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 85 |
| creator | Zhang, Qian Wang, Yiqi Meng, Xianyang Dhar, Rik Huang, Haidong |
| description | 8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A·OG-C triad or a C·OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration. |
| doi_str_mv | 10.1021/ac3033323 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1273114358</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2857274401</sourcerecordid><originalsourceid>FETCH-LOGICAL-a409t-d9cbb80e9991664a715fa710f184c3ff03631f9f971f4dff06c13ff911d5b58c3</originalsourceid><addsrcrecordid>eNptkdtqFTEUhoModlu98AUkIIIFo1nJHBLvNrseCsVetF4P2TnspswkYzIj3Xd9BR_Al_CR-iRN2bWIerPCWnx8f-BH6DnQt0AZvFOaU8454w_QAmpGSSMEe4gWlFJOWEvpHnqS8wWlABSax2iPFbatWrFAP8-SH3tLTqekgrEGH35Z4lUMk_LBhw0W11c_Ti4jad8IYvz51qRI2PXVL2JsvNxuZhVi9sG-x0fD2HutJh8D9gFP5xYf2uw3AUeHT21v9eS_W7wcJzXYVC4hx5Sxi-l_Gbfion2KHjnVZ_vs7t1HXz9-OFt9Jscnn45Wy2OiKionYqRerwW1Ukpomkq1ULsyqANRae4c5Q0HJ51swVWm7I2GcpYApl7XQvN99HrnHVP8Nts8dYPP2va9CjbOuQPWcoCK16KgL_9CL-KcQvldoUo4Z1UjC3Wwo3SKOSfrujH5QaVtB7S77ay776ywL-6M83qw5p78XVIBXu0ApfMfaf-IbgCFmp73</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1266432469</pqid></control><display><type>article</type><title>Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine</title><source>MEDLINE</source><source>ACS Publications</source><creator>Zhang, Qian ; Wang, Yiqi ; Meng, Xianyang ; Dhar, Rik ; Huang, Haidong</creator><creatorcontrib>Zhang, Qian ; Wang, Yiqi ; Meng, Xianyang ; Dhar, Rik ; Huang, Haidong</creatorcontrib><description>8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A·OG-C triad or a C·OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac3033323</identifier><identifier>PMID: 23237478</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Aptamers, Nucleotide - chemistry ; Biomarkers ; Biosensing Techniques ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - analysis ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; Fluorescence ; Fluorescent Dyes - chemistry ; Sensors ; Spectrometry, Fluorescence ; Transition Temperature ; Ultraviolet Rays</subject><ispartof>Analytical chemistry (Washington), 2013-01, Vol.85 (1), p.201-207</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>Copyright American Chemical Society Jan 2, 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a409t-d9cbb80e9991664a715fa710f184c3ff03631f9f971f4dff06c13ff911d5b58c3</citedby><cites>FETCH-LOGICAL-a409t-d9cbb80e9991664a715fa710f184c3ff03631f9f971f4dff06c13ff911d5b58c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac3033323$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac3033323$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23237478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Wang, Yiqi</creatorcontrib><creatorcontrib>Meng, Xianyang</creatorcontrib><creatorcontrib>Dhar, Rik</creatorcontrib><creatorcontrib>Huang, Haidong</creatorcontrib><title>Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A·OG-C triad or a C·OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration.</description><subject>Analytical chemistry</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biomarkers</subject><subject>Biosensing Techniques</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Sensors</subject><subject>Spectrometry, Fluorescence</subject><subject>Transition Temperature</subject><subject>Ultraviolet Rays</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkdtqFTEUhoModlu98AUkIIIFo1nJHBLvNrseCsVetF4P2TnspswkYzIj3Xd9BR_Al_CR-iRN2bWIerPCWnx8f-BH6DnQt0AZvFOaU8454w_QAmpGSSMEe4gWlFJOWEvpHnqS8wWlABSax2iPFbatWrFAP8-SH3tLTqekgrEGH35Z4lUMk_LBhw0W11c_Ti4jad8IYvz51qRI2PXVL2JsvNxuZhVi9sG-x0fD2HutJh8D9gFP5xYf2uw3AUeHT21v9eS_W7wcJzXYVC4hx5Sxi-l_Gbfion2KHjnVZ_vs7t1HXz9-OFt9Jscnn45Wy2OiKionYqRerwW1Ukpomkq1ULsyqANRae4c5Q0HJ51swVWm7I2GcpYApl7XQvN99HrnHVP8Nts8dYPP2va9CjbOuQPWcoCK16KgL_9CL-KcQvldoUo4Z1UjC3Wwo3SKOSfrujH5QaVtB7S77ay776ywL-6M83qw5p78XVIBXu0ApfMfaf-IbgCFmp73</recordid><startdate>20130102</startdate><enddate>20130102</enddate><creator>Zhang, Qian</creator><creator>Wang, Yiqi</creator><creator>Meng, Xianyang</creator><creator>Dhar, Rik</creator><creator>Huang, Haidong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20130102</creationdate><title>Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine</title><author>Zhang, Qian ; Wang, Yiqi ; Meng, Xianyang ; Dhar, Rik ; Huang, Haidong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a409t-d9cbb80e9991664a715fa710f184c3ff03631f9f971f4dff06c13ff911d5b58c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analytical chemistry</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Biomarkers</topic><topic>Biosensing Techniques</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - analysis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Sensors</topic><topic>Spectrometry, Fluorescence</topic><topic>Transition Temperature</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qian</creatorcontrib><creatorcontrib>Wang, Yiqi</creatorcontrib><creatorcontrib>Meng, Xianyang</creatorcontrib><creatorcontrib>Dhar, Rik</creatorcontrib><creatorcontrib>Huang, Haidong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qian</au><au>Wang, Yiqi</au><au>Meng, Xianyang</au><au>Dhar, Rik</au><au>Huang, Haidong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2013-01-02</date><risdate>2013</risdate><volume>85</volume><issue>1</issue><spage>201</spage><epage>207</epage><pages>201-207</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A·OG-C triad or a C·OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>23237478</pmid><doi>10.1021/ac3033323</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2013-01, Vol.85 (1), p.201-207 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1273114358 |
| source | MEDLINE; ACS Publications |
| subjects | Analytical chemistry Aptamers, Nucleotide - chemistry Biomarkers Biosensing Techniques Deoxyguanosine - analogs & derivatives Deoxyguanosine - analysis Deoxyribonucleic acid DNA DNA - chemistry Fluorescence Fluorescent Dyes - chemistry Sensors Spectrometry, Fluorescence Transition Temperature Ultraviolet Rays |
| title | Triple-Stranded DNA Containing 8‑Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8‑Oxo-7,8-dihydroguanine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T11%3A43%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Triple-Stranded%20DNA%20Containing%208%E2%80%91Oxo-7,8-dihydro-2%E2%80%B2-deoxyguanosine:%20Implication%20in%20the%20Design%20of%20Selective%20Aptamer%20Sensors%20for%208%E2%80%91Oxo-7,8-dihydroguanine&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Zhang,%20Qian&rft.date=2013-01-02&rft.volume=85&rft.issue=1&rft.spage=201&rft.epage=207&rft.pages=201-207&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac3033323&rft_dat=%3Cproquest_cross%3E2857274401%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1266432469&rft_id=info:pmid/23237478&rfr_iscdi=true |