Mycobacterium tuberculosis volatiles for diagnosis of tuberculosis by Cricetomys rats
Summary Tuberculosis (TB) diagnosis in regions with limited resources depends on microscopy with insufficient sensitivity. Rapid diagnostic tests of low cost but high sensitivity and specificity are needed for better point-of-care management of TB. Trained African giant pouched rats ( Cricetomys sp....
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2012-11, Vol.92 (6), p.535-542 |
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| creator | Mgode, Georgies F Weetjens, Bart J Nawrath, Thorben Lazar, Doris Cox, Christophe Jubitana, Maureen Mahoney, Amanda Kuipers, Dian Machang'u, Robert S Weiner, January Schulz, Stefan Kaufmann, Stefan H.E |
| description | Summary Tuberculosis (TB) diagnosis in regions with limited resources depends on microscopy with insufficient sensitivity. Rapid diagnostic tests of low cost but high sensitivity and specificity are needed for better point-of-care management of TB. Trained African giant pouched rats ( Cricetomys sp.) can diagnose pulmonary TB in sputum but the relevant Mycobacterium tuberculosis ( Mtb )-specific volatile compounds remain unknown. We investigated the odour volatiles of Mtb detected by rats in reference Mtb , nontuberculous mycobacteria, Nocardia sp., Streptomyces sp., Rhodococcus sp., and other respiratory tract microorganisms spiked into Mtb -negative sputum. Thirteen compounds were specific to Mtb and 13 were shared with other microorganisms. Rats discriminated a blend of Mtb -specific volatiles from individual, and blends of shared, compounds ( P = 0.001). The rats' sensitivity for typical TB-positive sputa was 99.15% with 92.23% specificity and 93.14% accuracy. These findings underline the potential of trained Cricetomys rats for rapid TB diagnosis in resource-limited settings, particularly in Africa where Cricetomys rats occur widely and the burden of TB is high. |
| doi_str_mv | 10.1016/j.tube.2012.07.006 |
| format | Article |
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Rapid diagnostic tests of low cost but high sensitivity and specificity are needed for better point-of-care management of TB. Trained African giant pouched rats ( Cricetomys sp.) can diagnose pulmonary TB in sputum but the relevant Mycobacterium tuberculosis ( Mtb )-specific volatile compounds remain unknown. We investigated the odour volatiles of Mtb detected by rats in reference Mtb , nontuberculous mycobacteria, Nocardia sp., Streptomyces sp., Rhodococcus sp., and other respiratory tract microorganisms spiked into Mtb -negative sputum. Thirteen compounds were specific to Mtb and 13 were shared with other microorganisms. Rats discriminated a blend of Mtb -specific volatiles from individual, and blends of shared, compounds ( P = 0.001). The rats' sensitivity for typical TB-positive sputa was 99.15% with 92.23% specificity and 93.14% accuracy. These findings underline the potential of trained Cricetomys rats for rapid TB diagnosis in resource-limited settings, particularly in Africa where Cricetomys rats occur widely and the burden of TB is high.</description><identifier>ISSN: 1472-9792</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2012.07.006</identifier><identifier>PMID: 22883935</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>African giant pouched rats ; Animals ; Bacterial Typing Techniques - methods ; Behavior, Animal - physiology ; Cricetinae ; Diagnosis, Differential ; Early tuberculosis diagnosis ; Infectious Disease ; Lung ; Microorganisms ; Microscopy ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - chemistry ; Mycobacterium volatile markers ; Nocardia ; Nocardia - chemistry ; Odor ; Odorants - analysis ; Olfactory detection ; Pulmonary/Respiratory ; Reproducibility of Results ; Respiratory tract ; Rhodococcus ; Rhodococcus - chemistry ; Sensitivity and Specificity ; Sputum ; Sputum - chemistry ; Sputum - microbiology ; Streptomyces ; Streptomyces - chemistry ; Tuberculosis ; Tuberculosis - diagnosis ; Volatiles ; Volatilization</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2012-11, Vol.92 (6), p.535-542</ispartof><rights>Elsevier Ltd</rights><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. 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Rapid diagnostic tests of low cost but high sensitivity and specificity are needed for better point-of-care management of TB. Trained African giant pouched rats ( Cricetomys sp.) can diagnose pulmonary TB in sputum but the relevant Mycobacterium tuberculosis ( Mtb )-specific volatile compounds remain unknown. We investigated the odour volatiles of Mtb detected by rats in reference Mtb , nontuberculous mycobacteria, Nocardia sp., Streptomyces sp., Rhodococcus sp., and other respiratory tract microorganisms spiked into Mtb -negative sputum. Thirteen compounds were specific to Mtb and 13 were shared with other microorganisms. Rats discriminated a blend of Mtb -specific volatiles from individual, and blends of shared, compounds ( P = 0.001). The rats' sensitivity for typical TB-positive sputa was 99.15% with 92.23% specificity and 93.14% accuracy. 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Weetjens, Bart J ; Nawrath, Thorben ; Lazar, Doris ; Cox, Christophe ; Jubitana, Maureen ; Mahoney, Amanda ; Kuipers, Dian ; Machang'u, Robert S ; Weiner, January ; Schulz, Stefan ; Kaufmann, Stefan H.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-68577a80ef6dd9acc58eedc8156a894a390dfae1423a55000542bfba61e687613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>African giant pouched rats</topic><topic>Animals</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Behavior, Animal - physiology</topic><topic>Cricetinae</topic><topic>Diagnosis, Differential</topic><topic>Early tuberculosis diagnosis</topic><topic>Infectious Disease</topic><topic>Lung</topic><topic>Microorganisms</topic><topic>Microscopy</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - chemistry</topic><topic>Mycobacterium volatile markers</topic><topic>Nocardia</topic><topic>Nocardia - chemistry</topic><topic>Odor</topic><topic>Odorants - analysis</topic><topic>Olfactory detection</topic><topic>Pulmonary/Respiratory</topic><topic>Reproducibility of Results</topic><topic>Respiratory tract</topic><topic>Rhodococcus</topic><topic>Rhodococcus - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Sputum</topic><topic>Sputum - chemistry</topic><topic>Sputum - microbiology</topic><topic>Streptomyces</topic><topic>Streptomyces - chemistry</topic><topic>Tuberculosis</topic><topic>Tuberculosis - diagnosis</topic><topic>Volatiles</topic><topic>Volatilization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mgode, Georgies F</creatorcontrib><creatorcontrib>Weetjens, Bart J</creatorcontrib><creatorcontrib>Nawrath, Thorben</creatorcontrib><creatorcontrib>Lazar, Doris</creatorcontrib><creatorcontrib>Cox, Christophe</creatorcontrib><creatorcontrib>Jubitana, Maureen</creatorcontrib><creatorcontrib>Mahoney, Amanda</creatorcontrib><creatorcontrib>Kuipers, Dian</creatorcontrib><creatorcontrib>Machang'u, Robert S</creatorcontrib><creatorcontrib>Weiner, January</creatorcontrib><creatorcontrib>Schulz, Stefan</creatorcontrib><creatorcontrib>Kaufmann, Stefan H.E</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mgode, Georgies F</au><au>Weetjens, Bart J</au><au>Nawrath, Thorben</au><au>Lazar, Doris</au><au>Cox, Christophe</au><au>Jubitana, Maureen</au><au>Mahoney, Amanda</au><au>Kuipers, Dian</au><au>Machang'u, Robert S</au><au>Weiner, January</au><au>Schulz, Stefan</au><au>Kaufmann, Stefan H.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacterium tuberculosis volatiles for diagnosis of tuberculosis by Cricetomys rats</atitle><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle><addtitle>Tuberculosis (Edinb)</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>92</volume><issue>6</issue><spage>535</spage><epage>542</epage><pages>535-542</pages><issn>1472-9792</issn><eissn>1873-281X</eissn><abstract>Summary Tuberculosis (TB) diagnosis in regions with limited resources depends on microscopy with insufficient sensitivity. Rapid diagnostic tests of low cost but high sensitivity and specificity are needed for better point-of-care management of TB. Trained African giant pouched rats ( Cricetomys sp.) can diagnose pulmonary TB in sputum but the relevant Mycobacterium tuberculosis ( Mtb )-specific volatile compounds remain unknown. We investigated the odour volatiles of Mtb detected by rats in reference Mtb , nontuberculous mycobacteria, Nocardia sp., Streptomyces sp., Rhodococcus sp., and other respiratory tract microorganisms spiked into Mtb -negative sputum. Thirteen compounds were specific to Mtb and 13 were shared with other microorganisms. Rats discriminated a blend of Mtb -specific volatiles from individual, and blends of shared, compounds ( P = 0.001). The rats' sensitivity for typical TB-positive sputa was 99.15% with 92.23% specificity and 93.14% accuracy. These findings underline the potential of trained Cricetomys rats for rapid TB diagnosis in resource-limited settings, particularly in Africa where Cricetomys rats occur widely and the burden of TB is high.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>22883935</pmid><doi>10.1016/j.tube.2012.07.006</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | African giant pouched rats Animals Bacterial Typing Techniques - methods Behavior, Animal - physiology Cricetinae Diagnosis, Differential Early tuberculosis diagnosis Infectious Disease Lung Microorganisms Microscopy Mycobacterium tuberculosis Mycobacterium tuberculosis - chemistry Mycobacterium volatile markers Nocardia Nocardia - chemistry Odor Odorants - analysis Olfactory detection Pulmonary/Respiratory Reproducibility of Results Respiratory tract Rhodococcus Rhodococcus - chemistry Sensitivity and Specificity Sputum Sputum - chemistry Sputum - microbiology Streptomyces Streptomyces - chemistry Tuberculosis Tuberculosis - diagnosis Volatiles Volatilization |
| title | Mycobacterium tuberculosis volatiles for diagnosis of tuberculosis by Cricetomys rats |
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