Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro
► Microcystin-LR (MCLR) can induce reproductive toxic effects on male Rana nigromaculata in vitro. ► MCLR can induce distention of mitochondria, endoplasmic reticulum, and Golgi apparatus in the testis of R. nigromaculata. ► The antioxidant defense system of the testis in R. nigromaculata can quickl...
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| Veröffentlicht in: | Aquatic toxicology 2013-01, Vol.126 (15), p.283-290 |
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| creator | Zhang, Hangjun Cai, Chenchen Wu, Yingzhu Ye, Binhui Han, Li Shou, Xiaolu Wang, Mengdi Wang, Jia Jia, Xiuying |
| description | ► Microcystin-LR (MCLR) can induce reproductive toxic effects on male Rana nigromaculata in vitro. ► MCLR can induce distention of mitochondria, endoplasmic reticulum, and Golgi apparatus in the testis of R. nigromaculata. ► The antioxidant defense system of the testis in R. nigromaculata can quickly respond to oxidative stress induced by MCLR. ► Expressions of P450 aromatase and SF-1 genes are important toxic mechanisms of male R. nigromaculata exposed to MCLR.
This study aims to demonstrate that microcystin-LR (MC-LR) has toxic effects on the reproductive system of male Rana nigromaculata in vitro. R. nigromaculata were treated with 0, 0.1, 1, 10, and 100nmol/L of MC-LR for 6h. Results show that exposure to 1nmol/L to 100nmol/L of MC-LR decreased sperm motility and number of sperm cells and increased the sperm abnormality rate, whose values were significantly different from those of the control (P |
| doi_str_mv | 10.1016/j.aquatox.2012.09.017 |
| format | Article |
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This study aims to demonstrate that microcystin-LR (MC-LR) has toxic effects on the reproductive system of male Rana nigromaculata in vitro. R. nigromaculata were treated with 0, 0.1, 1, 10, and 100nmol/L of MC-LR for 6h. Results show that exposure to 1nmol/L to 100nmol/L of MC-LR decreased sperm motility and number of sperm cells and increased the sperm abnormality rate, whose values were significantly different from those of the control (P<0.01). Moreover, the same dosage of MC-LR increased reactive oxygen species production and malondialdehyde content. At the same time, antioxidant enzyme (catalase and glutathione S-transferase) activity and glutathione reduced content rapidly increased, whereas antioxidant enzyme superoxide dismutase activity significantly decreased. These results imply that the defense system of the testes quickly responds to oxidative stress. Ultrastructural observation shows distention of the mitochondria, endoplasmic reticulum, and Golgi apparatus and changes in the mitochondrial matrix color, cristae number, and morphology. Moreover, using real-time PCR, increased relative expressions of P450 aromatase and SF-1 genes were observed. The results demonstrate for the first time that MC-LR could induce toxicity in the male reproductive system of R. nigromaculata. The findings in this research will provide more insights into the relationships between aquatic microcystins and amphibians.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2012.09.017</identifier><identifier>PMID: 23084047</identifier><identifier>CODEN: AQTODG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amphibia. Reptilia ; amphibians ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; catalase ; color ; Ecotoxicology, biological effects of pollution ; endoplasmic reticulum ; Enzyme Activation - drug effects ; Enzymes - metabolism ; Frog ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; General aspects ; genes ; Genitalia, Male - drug effects ; glutathione ; glutathione transferase ; Golgi apparatus ; Male ; malondialdehyde ; Malondialdehyde - metabolism ; MCLR ; microcystin-LR ; Microcystins - toxicity ; Microscopy, Electron, Transmission ; mitochondria ; oxidative stress ; quantitative polymerase chain reaction ; Rana ; Rana nigromaculata ; Ranidae ; reactive oxygen species ; Reactive Oxygen Species - metabolism ; Reproductive toxicity ; Sertoli Cells - drug effects ; sperm motility ; spermatozoa ; Spermatozoa - drug effects ; superoxide dismutase ; testes ; Testis ; toxicity ; unspecific monooxygenase ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution ; Water Pollutants, Chemical - toxicity</subject><ispartof>Aquatic toxicology, 2013-01, Vol.126 (15), p.283-290</ispartof><rights>2012 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-22b3c7165080fb876cfd8b04416b68f981de5bcade346c047956e24ab9e1af153</citedby><cites>FETCH-LOGICAL-c452t-22b3c7165080fb876cfd8b04416b68f981de5bcade346c047956e24ab9e1af153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2012.09.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26830998$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23084047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hangjun</creatorcontrib><creatorcontrib>Cai, Chenchen</creatorcontrib><creatorcontrib>Wu, Yingzhu</creatorcontrib><creatorcontrib>Ye, Binhui</creatorcontrib><creatorcontrib>Han, Li</creatorcontrib><creatorcontrib>Shou, Xiaolu</creatorcontrib><creatorcontrib>Wang, Mengdi</creatorcontrib><creatorcontrib>Wang, Jia</creatorcontrib><creatorcontrib>Jia, Xiuying</creatorcontrib><title>Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>► Microcystin-LR (MCLR) can induce reproductive toxic effects on male Rana nigromaculata in vitro. ► MCLR can induce distention of mitochondria, endoplasmic reticulum, and Golgi apparatus in the testis of R. nigromaculata. ► The antioxidant defense system of the testis in R. nigromaculata can quickly respond to oxidative stress induced by MCLR. ► Expressions of P450 aromatase and SF-1 genes are important toxic mechanisms of male R. nigromaculata exposed to MCLR.
This study aims to demonstrate that microcystin-LR (MC-LR) has toxic effects on the reproductive system of male Rana nigromaculata in vitro. R. nigromaculata were treated with 0, 0.1, 1, 10, and 100nmol/L of MC-LR for 6h. Results show that exposure to 1nmol/L to 100nmol/L of MC-LR decreased sperm motility and number of sperm cells and increased the sperm abnormality rate, whose values were significantly different from those of the control (P<0.01). Moreover, the same dosage of MC-LR increased reactive oxygen species production and malondialdehyde content. At the same time, antioxidant enzyme (catalase and glutathione S-transferase) activity and glutathione reduced content rapidly increased, whereas antioxidant enzyme superoxide dismutase activity significantly decreased. These results imply that the defense system of the testes quickly responds to oxidative stress. Ultrastructural observation shows distention of the mitochondria, endoplasmic reticulum, and Golgi apparatus and changes in the mitochondrial matrix color, cristae number, and morphology. Moreover, using real-time PCR, increased relative expressions of P450 aromatase and SF-1 genes were observed. The results demonstrate for the first time that MC-LR could induce toxicity in the male reproductive system of R. nigromaculata. The findings in this research will provide more insights into the relationships between aquatic microcystins and amphibians.</description><subject>Amphibia. Reptilia</subject><subject>amphibians</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>catalase</subject><subject>color</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>endoplasmic reticulum</subject><subject>Enzyme Activation - drug effects</subject><subject>Enzymes - metabolism</subject><subject>Frog</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>General aspects</subject><subject>genes</subject><subject>Genitalia, Male - drug effects</subject><subject>glutathione</subject><subject>glutathione transferase</subject><subject>Golgi apparatus</subject><subject>Male</subject><subject>malondialdehyde</subject><subject>Malondialdehyde - metabolism</subject><subject>MCLR</subject><subject>microcystin-LR</subject><subject>Microcystins - toxicity</subject><subject>Microscopy, Electron, Transmission</subject><subject>mitochondria</subject><subject>oxidative stress</subject><subject>quantitative polymerase chain reaction</subject><subject>Rana</subject><subject>Rana nigromaculata</subject><subject>Ranidae</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Reproductive toxicity</subject><subject>Sertoli Cells - drug effects</subject><subject>sperm motility</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>superoxide dismutase</subject><subject>testes</subject><subject>Testis</subject><subject>toxicity</subject><subject>unspecific monooxygenase</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCTwB8QeKS1E4cxzmhqoJSaSWkfkicsCbOuHiVxK3trNp_j5ddyhFffPAznneeIeQdZyVnXJ5uSnhYIPnHsmK8KllXMt6-ICuu2q7gDRcvySpzshCi-XFEjmPcsHwq0b0mR1XNlGCiXZGfN_7RGYrWokmReksnZ4I3TzG5uVhfUT_T9AtpwPvgh8Ukt0Ua8ytOf2AYkV7BDHR2d8FPYJYRElA3061Lwb8hryyMEd8e7hNy-_XLzfm3Yv394vL8bF0Y0VSpqKq-Ni2XDVPM9qqVxg6qZ0Jw2UtlO8UHbHoDA9ZCmhy8ayRWAvoOOVje1Cfk0_7fnPJhwZj05KLBcYQZ_RI1r6SSDZeszmizR_OYMQa0-j64CcKT5kzv1OqNPqjVO7WadTqrzXXvDy2WfsLhueqvywx8PAAQDYw2wGxc_MdJVbOuU5n7sOcseA13ITO317mTYIyrvL0d8XlPYFa2dRh0NA5ng4MLeU168O4_YX8DDB-j7Q</recordid><startdate>20130115</startdate><enddate>20130115</enddate><creator>Zhang, Hangjun</creator><creator>Cai, Chenchen</creator><creator>Wu, Yingzhu</creator><creator>Ye, Binhui</creator><creator>Han, Li</creator><creator>Shou, Xiaolu</creator><creator>Wang, Mengdi</creator><creator>Wang, Jia</creator><creator>Jia, Xiuying</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>SOI</scope></search><sort><creationdate>20130115</creationdate><title>Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro</title><author>Zhang, Hangjun ; Cai, Chenchen ; Wu, Yingzhu ; Ye, Binhui ; Han, Li ; Shou, Xiaolu ; Wang, Mengdi ; Wang, Jia ; Jia, Xiuying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-22b3c7165080fb876cfd8b04416b68f981de5bcade346c047956e24ab9e1af153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amphibia. Reptilia</topic><topic>amphibians</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>catalase</topic><topic>color</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>endoplasmic reticulum</topic><topic>Enzyme Activation - drug effects</topic><topic>Enzymes - metabolism</topic><topic>Frog</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>General aspects</topic><topic>genes</topic><topic>Genitalia, Male - drug effects</topic><topic>glutathione</topic><topic>glutathione transferase</topic><topic>Golgi apparatus</topic><topic>Male</topic><topic>malondialdehyde</topic><topic>Malondialdehyde - metabolism</topic><topic>MCLR</topic><topic>microcystin-LR</topic><topic>Microcystins - toxicity</topic><topic>Microscopy, Electron, Transmission</topic><topic>mitochondria</topic><topic>oxidative stress</topic><topic>quantitative polymerase chain reaction</topic><topic>Rana</topic><topic>Rana nigromaculata</topic><topic>Ranidae</topic><topic>reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Reproductive toxicity</topic><topic>Sertoli Cells - drug effects</topic><topic>sperm motility</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>superoxide dismutase</topic><topic>testes</topic><topic>Testis</topic><topic>toxicity</topic><topic>unspecific monooxygenase</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hangjun</creatorcontrib><creatorcontrib>Cai, Chenchen</creatorcontrib><creatorcontrib>Wu, Yingzhu</creatorcontrib><creatorcontrib>Ye, Binhui</creatorcontrib><creatorcontrib>Han, Li</creatorcontrib><creatorcontrib>Shou, Xiaolu</creatorcontrib><creatorcontrib>Wang, Mengdi</creatorcontrib><creatorcontrib>Wang, Jia</creatorcontrib><creatorcontrib>Jia, Xiuying</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environment Abstracts</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hangjun</au><au>Cai, Chenchen</au><au>Wu, Yingzhu</au><au>Ye, Binhui</au><au>Han, Li</au><au>Shou, Xiaolu</au><au>Wang, Mengdi</au><au>Wang, Jia</au><au>Jia, Xiuying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2013-01-15</date><risdate>2013</risdate><volume>126</volume><issue>15</issue><spage>283</spage><epage>290</epage><pages>283-290</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><coden>AQTODG</coden><abstract>► Microcystin-LR (MCLR) can induce reproductive toxic effects on male Rana nigromaculata in vitro. ► MCLR can induce distention of mitochondria, endoplasmic reticulum, and Golgi apparatus in the testis of R. nigromaculata. ► The antioxidant defense system of the testis in R. nigromaculata can quickly respond to oxidative stress induced by MCLR. ► Expressions of P450 aromatase and SF-1 genes are important toxic mechanisms of male R. nigromaculata exposed to MCLR.
This study aims to demonstrate that microcystin-LR (MC-LR) has toxic effects on the reproductive system of male Rana nigromaculata in vitro. R. nigromaculata were treated with 0, 0.1, 1, 10, and 100nmol/L of MC-LR for 6h. Results show that exposure to 1nmol/L to 100nmol/L of MC-LR decreased sperm motility and number of sperm cells and increased the sperm abnormality rate, whose values were significantly different from those of the control (P<0.01). Moreover, the same dosage of MC-LR increased reactive oxygen species production and malondialdehyde content. At the same time, antioxidant enzyme (catalase and glutathione S-transferase) activity and glutathione reduced content rapidly increased, whereas antioxidant enzyme superoxide dismutase activity significantly decreased. These results imply that the defense system of the testes quickly responds to oxidative stress. Ultrastructural observation shows distention of the mitochondria, endoplasmic reticulum, and Golgi apparatus and changes in the mitochondrial matrix color, cristae number, and morphology. Moreover, using real-time PCR, increased relative expressions of P450 aromatase and SF-1 genes were observed. The results demonstrate for the first time that MC-LR could induce toxicity in the male reproductive system of R. nigromaculata. The findings in this research will provide more insights into the relationships between aquatic microcystins and amphibians.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23084047</pmid><doi>10.1016/j.aquatox.2012.09.017</doi><tpages>8</tpages></addata></record> |
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| ispartof | Aquatic toxicology, 2013-01, Vol.126 (15), p.283-290 |
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| subjects | Amphibia. Reptilia amphibians Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences catalase color Ecotoxicology, biological effects of pollution endoplasmic reticulum Enzyme Activation - drug effects Enzymes - metabolism Frog Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects General aspects genes Genitalia, Male - drug effects glutathione glutathione transferase Golgi apparatus Male malondialdehyde Malondialdehyde - metabolism MCLR microcystin-LR Microcystins - toxicity Microscopy, Electron, Transmission mitochondria oxidative stress quantitative polymerase chain reaction Rana Rana nigromaculata Ranidae reactive oxygen species Reactive Oxygen Species - metabolism Reproductive toxicity Sertoli Cells - drug effects sperm motility spermatozoa Spermatozoa - drug effects superoxide dismutase testes Testis toxicity unspecific monooxygenase Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Water Pollutants, Chemical - toxicity |
| title | Toxic effects of microcystin-LR on the reproductive system of male Rana nigromaculata in vitro |
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