Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells
► Farm rabbits in Inner Mongolia, China, were examined for HEV infection. ► Overall, 151 (71.6%) of 211 rabbits studied had detectable rabbit HEV. ► Three clusters of rabbit HEV strains were found within a single farm. ► Rabbit HEV strains propagated efficiently in A549 and PLC/PRF/5 cells. ► The po...
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| creator | Jirintai, Suljid Jinshan Tanggis Manglai, Dugarjavin Mulyanto Takahashi, Masaharu Nagashima, Shigeo Kobayashi, Tominari Nishizawa, Tsutomu Okamoto, Hiroaki |
| description | ► Farm rabbits in Inner Mongolia, China, were examined for HEV infection. ► Overall, 151 (71.6%) of 211 rabbits studied had detectable rabbit HEV. ► Three clusters of rabbit HEV strains were found within a single farm. ► Rabbit HEV strains propagated efficiently in A549 and PLC/PRF/5 cells. ► The potential zoonotic risk of rabbit HEV was suggested.
Rabbit hepatitis E virus (HEV) strains have recently been isolated in several areas of China and in the US and France. However, the host range, distribution and zoonotic potential of these HEV strains remain unknown and their propagation in cultured cells has not yet been reported. A total of 211 4-month-old rabbits raised on a farm in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 121 rabbits (57.3%) tested positive for anti-HEV antibodies, and 151 (71.6%) had detectable HEV RNA. The 174 HEV strains recovered from these viremic rabbits, including two distinct strains each from 23 rabbits, differed from each other by up to 13.6% in a 412-nucleotide (nt) sequence within ORF2, and were 89.3–95.9% identical to the reported rabbit HEV strains in other provinces of China. Three representative Inner Mongolian strains, one each from three phylogenetic clusters, whose entire genomic sequences were determined, shared 79.6–96.7% identities with reported rabbit HEV strains within the entire or 242- to 1349-nt partial genomic sequence. Rabbit HEV strains recovered from liver tissues of rabbits with a high HEV load propagated efficiently in human cell lines (A549 and PLC/PRF/5 cells), suggesting the potential zoonotic risk of rabbit HEV. |
| doi_str_mv | 10.1016/j.virusres.2012.09.015 |
| format | Article |
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Rabbit hepatitis E virus (HEV) strains have recently been isolated in several areas of China and in the US and France. However, the host range, distribution and zoonotic potential of these HEV strains remain unknown and their propagation in cultured cells has not yet been reported. A total of 211 4-month-old rabbits raised on a farm in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 121 rabbits (57.3%) tested positive for anti-HEV antibodies, and 151 (71.6%) had detectable HEV RNA. The 174 HEV strains recovered from these viremic rabbits, including two distinct strains each from 23 rabbits, differed from each other by up to 13.6% in a 412-nucleotide (nt) sequence within ORF2, and were 89.3–95.9% identical to the reported rabbit HEV strains in other provinces of China. Three representative Inner Mongolian strains, one each from three phylogenetic clusters, whose entire genomic sequences were determined, shared 79.6–96.7% identities with reported rabbit HEV strains within the entire or 242- to 1349-nt partial genomic sequence. Rabbit HEV strains recovered from liver tissues of rabbits with a high HEV load propagated efficiently in human cell lines (A549 and PLC/PRF/5 cells), suggesting the potential zoonotic risk of rabbit HEV.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2012.09.015</identifier><identifier>PMID: 23041252</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies ; Cell culture ; Cell Line ; China ; Farms ; Genetic Heterogeneity ; Genome, Viral ; genomics ; Hepatitis Antibodies - blood ; Hepatitis Antibodies - immunology ; Hepatitis E virus ; Hepatitis E virus - genetics ; Hepatitis E virus - immunology ; Hepatitis E virus - physiology ; Hepatitis, Viral, Animal - epidemiology ; Hepatitis, Viral, Animal - immunology ; Hepatitis, Viral, Animal - virology ; Host range ; human cell lines ; Humans ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Inner Mongolia ; Liver ; nucleotide sequences ; Phylogeny ; Rabbit ; Rabbits ; risk ; RNA ; RNA, Viral ; Seroepidemiologic Studies ; Viral Proteins - genetics ; Virus Replication</subject><ispartof>Virus research, 2012-12, Vol.170 (1-2), p.126-137</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-a79eadd87ce3f5215c0581c65944c6cfe9331e5394da392147cf5ad7621847123</citedby><cites>FETCH-LOGICAL-c491t-a79eadd87ce3f5215c0581c65944c6cfe9331e5394da392147cf5ad7621847123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2012.09.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23041252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jirintai, Suljid</creatorcontrib><creatorcontrib>Jinshan</creatorcontrib><creatorcontrib>Tanggis</creatorcontrib><creatorcontrib>Manglai, Dugarjavin</creatorcontrib><creatorcontrib>Mulyanto</creatorcontrib><creatorcontrib>Takahashi, Masaharu</creatorcontrib><creatorcontrib>Nagashima, Shigeo</creatorcontrib><creatorcontrib>Kobayashi, Tominari</creatorcontrib><creatorcontrib>Nishizawa, Tsutomu</creatorcontrib><creatorcontrib>Okamoto, Hiroaki</creatorcontrib><title>Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>► Farm rabbits in Inner Mongolia, China, were examined for HEV infection. ► Overall, 151 (71.6%) of 211 rabbits studied had detectable rabbit HEV. ► Three clusters of rabbit HEV strains were found within a single farm. ► Rabbit HEV strains propagated efficiently in A549 and PLC/PRF/5 cells. ► The potential zoonotic risk of rabbit HEV was suggested.
Rabbit hepatitis E virus (HEV) strains have recently been isolated in several areas of China and in the US and France. However, the host range, distribution and zoonotic potential of these HEV strains remain unknown and their propagation in cultured cells has not yet been reported. A total of 211 4-month-old rabbits raised on a farm in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 121 rabbits (57.3%) tested positive for anti-HEV antibodies, and 151 (71.6%) had detectable HEV RNA. The 174 HEV strains recovered from these viremic rabbits, including two distinct strains each from 23 rabbits, differed from each other by up to 13.6% in a 412-nucleotide (nt) sequence within ORF2, and were 89.3–95.9% identical to the reported rabbit HEV strains in other provinces of China. Three representative Inner Mongolian strains, one each from three phylogenetic clusters, whose entire genomic sequences were determined, shared 79.6–96.7% identities with reported rabbit HEV strains within the entire or 242- to 1349-nt partial genomic sequence. Rabbit HEV strains recovered from liver tissues of rabbits with a high HEV load propagated efficiently in human cell lines (A549 and PLC/PRF/5 cells), suggesting the potential zoonotic risk of rabbit HEV.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>China</subject><subject>Farms</subject><subject>Genetic Heterogeneity</subject><subject>Genome, Viral</subject><subject>genomics</subject><subject>Hepatitis Antibodies - blood</subject><subject>Hepatitis Antibodies - immunology</subject><subject>Hepatitis E virus</subject><subject>Hepatitis E virus - genetics</subject><subject>Hepatitis E virus - immunology</subject><subject>Hepatitis E virus - physiology</subject><subject>Hepatitis, Viral, Animal - epidemiology</subject><subject>Hepatitis, Viral, Animal - immunology</subject><subject>Hepatitis, Viral, Animal - virology</subject><subject>Host range</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>Inner Mongolia</subject><subject>Liver</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Rabbit</subject><subject>Rabbits</subject><subject>risk</subject><subject>RNA</subject><subject>RNA, Viral</subject><subject>Seroepidemiologic Studies</subject><subject>Viral Proteins - genetics</subject><subject>Virus Replication</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFO3DAURa2qFUwpv0C97IJkbMeO413RCFqkQUVtWVse53nwKImndoLER_DPOAx0264sS-fe9_QOQmeUlJTQerkrH3ycUoRUMkJZSVRJqHiHFrSRrJBcsfdokcGmoJKwY_QxpR0hpK5kfYSOWUU4ZYIt0NNN6MBOnYnYDKZ7TD7h4PA97M3ox_y5xC-DsIuhx87EHkez2fgxYT_g62GAiG_CsA2dN-d4de8Hk4taPANpshZSclOH9zHszTZXhmHOXQiuXrDb9Wp5-_NqKbCFrkuf0AdnugSnr-8Juru6_L36Xqx_fLteXawLyxUdCyMVmLZtpIXKCUaFJaKhthaKc1tbB6qqKIhK8dZUilEurROmlTWjDZeUVSfoy6E37_VngjTq3qd5AzNAmJLOt5GSU0LEf6C5NI-qZUbrA2pjSFmN0_voexMfNSV6tqZ3-s2anq1ponS2loNnrzOmTQ_t39ibpgx8PgDOBG220Sd99ys31FkpYUrRTHw9EJDP9uAh6mQ9DBZaH8GOug3-X1s8AwQdtIo</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Jirintai, Suljid</creator><creator>Jinshan</creator><creator>Tanggis</creator><creator>Manglai, Dugarjavin</creator><creator>Mulyanto</creator><creator>Takahashi, Masaharu</creator><creator>Nagashima, Shigeo</creator><creator>Kobayashi, Tominari</creator><creator>Nishizawa, Tsutomu</creator><creator>Okamoto, Hiroaki</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20121201</creationdate><title>Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells</title><author>Jirintai, Suljid ; Jinshan ; Tanggis ; Manglai, Dugarjavin ; Mulyanto ; Takahashi, Masaharu ; Nagashima, Shigeo ; Kobayashi, Tominari ; Nishizawa, Tsutomu ; Okamoto, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-a79eadd87ce3f5215c0581c65944c6cfe9331e5394da392147cf5ad7621847123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>China</topic><topic>Farms</topic><topic>Genetic Heterogeneity</topic><topic>Genome, Viral</topic><topic>genomics</topic><topic>Hepatitis Antibodies - blood</topic><topic>Hepatitis Antibodies - immunology</topic><topic>Hepatitis E virus</topic><topic>Hepatitis E virus - genetics</topic><topic>Hepatitis E virus - immunology</topic><topic>Hepatitis E virus - physiology</topic><topic>Hepatitis, Viral, Animal - epidemiology</topic><topic>Hepatitis, Viral, Animal - immunology</topic><topic>Hepatitis, Viral, Animal - virology</topic><topic>Host range</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - immunology</topic><topic>Inner Mongolia</topic><topic>Liver</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Rabbit</topic><topic>Rabbits</topic><topic>risk</topic><topic>RNA</topic><topic>RNA, Viral</topic><topic>Seroepidemiologic Studies</topic><topic>Viral Proteins - genetics</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jirintai, Suljid</creatorcontrib><creatorcontrib>Jinshan</creatorcontrib><creatorcontrib>Tanggis</creatorcontrib><creatorcontrib>Manglai, Dugarjavin</creatorcontrib><creatorcontrib>Mulyanto</creatorcontrib><creatorcontrib>Takahashi, Masaharu</creatorcontrib><creatorcontrib>Nagashima, Shigeo</creatorcontrib><creatorcontrib>Kobayashi, Tominari</creatorcontrib><creatorcontrib>Nishizawa, Tsutomu</creatorcontrib><creatorcontrib>Okamoto, Hiroaki</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jirintai, Suljid</au><au>Jinshan</au><au>Tanggis</au><au>Manglai, Dugarjavin</au><au>Mulyanto</au><au>Takahashi, Masaharu</au><au>Nagashima, Shigeo</au><au>Kobayashi, Tominari</au><au>Nishizawa, Tsutomu</au><au>Okamoto, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>170</volume><issue>1-2</issue><spage>126</spage><epage>137</epage><pages>126-137</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>► Farm rabbits in Inner Mongolia, China, were examined for HEV infection. ► Overall, 151 (71.6%) of 211 rabbits studied had detectable rabbit HEV. ► Three clusters of rabbit HEV strains were found within a single farm. ► Rabbit HEV strains propagated efficiently in A549 and PLC/PRF/5 cells. ► The potential zoonotic risk of rabbit HEV was suggested.
Rabbit hepatitis E virus (HEV) strains have recently been isolated in several areas of China and in the US and France. However, the host range, distribution and zoonotic potential of these HEV strains remain unknown and their propagation in cultured cells has not yet been reported. A total of 211 4-month-old rabbits raised on a farm in Inner Mongolia were tested for the presence of anti-HEV antibodies and HEV RNA. Overall, 121 rabbits (57.3%) tested positive for anti-HEV antibodies, and 151 (71.6%) had detectable HEV RNA. The 174 HEV strains recovered from these viremic rabbits, including two distinct strains each from 23 rabbits, differed from each other by up to 13.6% in a 412-nucleotide (nt) sequence within ORF2, and were 89.3–95.9% identical to the reported rabbit HEV strains in other provinces of China. Three representative Inner Mongolian strains, one each from three phylogenetic clusters, whose entire genomic sequences were determined, shared 79.6–96.7% identities with reported rabbit HEV strains within the entire or 242- to 1349-nt partial genomic sequence. Rabbit HEV strains recovered from liver tissues of rabbits with a high HEV load propagated efficiently in human cell lines (A549 and PLC/PRF/5 cells), suggesting the potential zoonotic risk of rabbit HEV.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23041252</pmid><doi>10.1016/j.virusres.2012.09.015</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Antibodies Cell culture Cell Line China Farms Genetic Heterogeneity Genome, Viral genomics Hepatitis Antibodies - blood Hepatitis Antibodies - immunology Hepatitis E virus Hepatitis E virus - genetics Hepatitis E virus - immunology Hepatitis E virus - physiology Hepatitis, Viral, Animal - epidemiology Hepatitis, Viral, Animal - immunology Hepatitis, Viral, Animal - virology Host range human cell lines Humans Immunoglobulin G - blood Immunoglobulin G - immunology Inner Mongolia Liver nucleotide sequences Phylogeny Rabbit Rabbits risk RNA RNA, Viral Seroepidemiologic Studies Viral Proteins - genetics Virus Replication |
| title | Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells |
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