Development of a cell-based high throughput luciferase enzyme fragment complementation assay to identify nuclear-factor-e2-related transcription factor 2 activators

Nuclear-factor-E2-related transcription factor 2 (Nrf2) regulates a large panel of Phase II genes and plays an important role in cell survival. Nrf2 activation has been shown as preventing cigarette smoke-induced alveolar enlargement in mice. Therefore, activation of the Nrf2 protein by small-molecu...

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Veröffentlicht in:	Assay and drug development technologies 2012-12, Vol.10 (6), p.514-524
Hauptverfasser:	Xie, Wensheng, Pao, Christina, Graham, Taylor, Dul, Ed, Lu, Quinn, Sweitzer, Thomas D, Ames, Robert S, Li, Hu
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Automation Cell Count Cloning, Molecular Core Binding Factor Alpha 1 Subunit - metabolism Culture Media Cytomegalovirus - genetics Data Interpretation, Statistical Dimethyl Sulfoxide - pharmacology Genetic Complementation Test - methods Genetic Vectors HEK293 Cells High-Throughput Screening Assays - methods Humans Luciferases - metabolism NF-E2-Related Factor 2 - agonists NF-E2-Related Factor 2 - drug effects Real-Time Polymerase Chain Reaction Small Molecule Libraries Transduction, Genetic
Online-Zugang:	Volltext
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