Quasi-Real Time Quantification of Uric Acid in Urine Using Boron Doped Diamond Microelectrode with in Situ Cleaning
We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2012-12, Vol.84 (23), p.10207-10213 |
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| creator | Kiran, Raphael Scorsone, Emmanuel Mailley, Pascal Bergonzo, Philippe |
| description | We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD’s superior properties such as low background current, low adsorption of species, long-term stability, and antifouling capabilities using electrochemical reactivation. Moreover, the article also describes an in situ activation technique, where the electrodes were reactivated within human urine, thereby opening the way toward automatic quantification of UA with in situ cleaning. The time taken to quantify UA concentration and cleaning remains below 0.5 s. Two analytic models were derived, based on different concentrations of ascorbic acid (AA) and uric acid, consisting of 2 s order calibration curves. Solving the second order equation enables the direct estimation of UA concentration, and values demonstrated good accuracy when compared with spectrophotometric measurements. |
| doi_str_mv | 10.1021/ac301177z |
| format | Article |
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On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD’s superior properties such as low background current, low adsorption of species, long-term stability, and antifouling capabilities using electrochemical reactivation. Moreover, the article also describes an in situ activation technique, where the electrodes were reactivated within human urine, thereby opening the way toward automatic quantification of UA with in situ cleaning. The time taken to quantify UA concentration and cleaning remains below 0.5 s. Two analytic models were derived, based on different concentrations of ascorbic acid (AA) and uric acid, consisting of 2 s order calibration curves. 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Chem</addtitle><description>We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD’s superior properties such as low background current, low adsorption of species, long-term stability, and antifouling capabilities using electrochemical reactivation. Moreover, the article also describes an in situ activation technique, where the electrodes were reactivated within human urine, thereby opening the way toward automatic quantification of UA with in situ cleaning. The time taken to quantify UA concentration and cleaning remains below 0.5 s. Two analytic models were derived, based on different concentrations of ascorbic acid (AA) and uric acid, consisting of 2 s order calibration curves. Solving the second order equation enables the direct estimation of UA concentration, and values demonstrated good accuracy when compared with spectrophotometric measurements.</description><subject>Acids</subject><subject>Analytical chemistry</subject><subject>Automation</subject><subject>Biosensing Techniques</subject><subject>Boron - chemistry</subject><subject>Chemistry</subject><subject>Diamond - chemistry</subject><subject>Electrocatalysis</subject><subject>Electrochemical methods</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Microelectrodes</subject><subject>Spectrometric and optical methods</subject><subject>Uric Acid - urine</subject><subject>Volumetric analysis</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0V9rFDEQAPAgir1WH_wCEhBBH1Yzye5m97Fe6x-oiNp7XuaSiabsJtdkF9FPb46erWhewsBvJjMZxp6AeAVCwms0SgBo_eseW0EjRdV2nbzPVkIIVUktxBE7zvlKFCSgfciOpALZ1o1Ysfx5weyrL4Qjv_QT8RKH2TtvcPYx8Oj4JnnDT4233Id9EIhvsg_f-JuYijiLO7L8zOMUg-UfvUmRRjJzipb4Dz9_36d99fPC1yNhKImP2AOHY6bHh_uEbd6eX67fVxef3n1Yn15UqLSaK6Whh77dth1Kiz0gkdYdtNpahBplQySbpq23fesaZ7WpXS87KRzauunIqhP24qbuLsXrhfI8TD4bGkcMFJc8gCxHqR6g0Gf_0Ku4pFC62yvVqU7IpqiXN6rMmHMiN-ySnzD9HEAM-00Mt5so9umh4rKdyN7KP19fwPMDwGxwdAmD8fnOtVpCrfWdQ5P_6uq_B38Dipuaug</recordid><startdate>20121204</startdate><enddate>20121204</enddate><creator>Kiran, Raphael</creator><creator>Scorsone, Emmanuel</creator><creator>Mailley, Pascal</creator><creator>Bergonzo, Philippe</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20121204</creationdate><title>Quasi-Real Time Quantification of Uric Acid in Urine Using Boron Doped Diamond Microelectrode with in Situ Cleaning</title><author>Kiran, Raphael ; Scorsone, Emmanuel ; Mailley, Pascal ; Bergonzo, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a373t-3719196b68a2da91aee778167dda14a25ee25564b96f5fd7c4f92820fad458ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acids</topic><topic>Analytical chemistry</topic><topic>Automation</topic><topic>Biosensing Techniques</topic><topic>Boron - chemistry</topic><topic>Chemistry</topic><topic>Diamond - chemistry</topic><topic>Electrocatalysis</topic><topic>Electrochemical methods</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Microelectrodes</topic><topic>Spectrometric and optical methods</topic><topic>Uric Acid - urine</topic><topic>Volumetric analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiran, Raphael</creatorcontrib><creatorcontrib>Scorsone, Emmanuel</creatorcontrib><creatorcontrib>Mailley, Pascal</creatorcontrib><creatorcontrib>Bergonzo, Philippe</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiran, Raphael</au><au>Scorsone, Emmanuel</au><au>Mailley, Pascal</au><au>Bergonzo, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quasi-Real Time Quantification of Uric Acid in Urine Using Boron Doped Diamond Microelectrode with in Situ Cleaning</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2012-12-04</date><risdate>2012</risdate><volume>84</volume><issue>23</issue><spage>10207</spage><epage>10213</epage><pages>10207-10213</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We report herein an innovative electrochemical (EC) technique based on boron doped diamond (BDD) microelectrodes which enable the fast determination of uric acid (UA) concentrations in urine. On the basis of fast cyclic voltammetry (CV), the technique was assessed in human urine samples and compared successfully using routine spectrophotometric diagnosis. The approach relies on the use of BDD’s superior properties such as low background current, low adsorption of species, long-term stability, and antifouling capabilities using electrochemical reactivation. 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| subjects | Acids Analytical chemistry Automation Biosensing Techniques Boron - chemistry Chemistry Diamond - chemistry Electrocatalysis Electrochemical methods Electrochemistry Electrodes Exact sciences and technology Humans Microelectrodes Spectrometric and optical methods Uric Acid - urine Volumetric analysis |
| title | Quasi-Real Time Quantification of Uric Acid in Urine Using Boron Doped Diamond Microelectrode with in Situ Cleaning |
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