Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach
Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to in...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2012, Vol.74(11), pp.1401-1407 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1407 |
|---|---|
| container_issue | 11 |
| container_start_page | 1401 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 74 |
| creator | SUNG, Jin-Hee CHO, Eun-Hae CHO, Jae-Hyeon WON, Chung-Kil KIM, Myeong-Ok KOH, Phil-Ok |
| description | Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD+], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes. |
| doi_str_mv | 10.1292/jvms.12-0063 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1222233232</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1222233232</sourcerecordid><originalsourceid>FETCH-LOGICAL-c635t-e08b0a07571dac868807c8c441d75c148b37b193ef5653e16fc0f3b34018877f3</originalsourceid><addsrcrecordid>eNpdkc2P1CAYh4nRuLOjN8-GxIsHq3y0hR6bietusps1Rs-EwttdJrSMQE3m4P8uY9c5CAm8gScPHz-E3lDykbKOfdr_mlKpKkJa_gxtKK9FJWrePUcb0tG2EqwhF-gypT0hjNZt9xJdMCZkQ5p2g37fWJizG53R2YUZhxF_jSGDmxP-Bg-L1xksHo74CuLincG9cRa7GWt856z1gHcQYYja4z5miEd8b4xf0snVz24q63fBgq_61RsmZxLuD4cYtHl8hV6M2id4_TRv0Y-rz99319Xt_ZebXX9bmZY3uQIiB6KJaAS12shWSiKMNHVNrWgMreXAxUA7DmPTNhxoOxoy8oHXhEopxMi36P3qLcf-XCBlNblkwHs9Q1iSoqw0zlnpW_TuP3QfljiX26nyeVIwTpgo1IeVMjGkFGFUh1geG4-KEnWKRZ1iKZU6xVLwt0_SZZjAnuF_ORRgtwL7lPUDnAEdszMeVpuoFaV_x1V73jWPOiqY-R_G6J_o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468723027</pqid></control><display><type>article</type><title>Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach</title><source>J-STAGE (Free - Japanese)</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>SUNG, Jin-Hee ; CHO, Eun-Hae ; CHO, Jae-Hyeon ; WON, Chung-Kil ; KIM, Myeong-Ok ; KOH, Phil-Ok</creator><creatorcontrib>SUNG, Jin-Hee ; CHO, Eun-Hae ; CHO, Jae-Hyeon ; WON, Chung-Kil ; KIM, Myeong-Ok ; KOH, Phil-Ok</creatorcontrib><description>Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD+], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.12-0063</identifier><identifier>PMID: 22785056</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Adenosylhomocysteinase - metabolism ; Animals ; Blotting, Western ; Brain - metabolism ; Brain Ischemia - drug therapy ; Brain Ischemia - etiology ; Brain Ischemia - metabolism ; Coumaric Acids - pharmacology ; DNA Primers - genetics ; Electrophoresis, Gel, Two-Dimensional ; ferulic acid ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Enzymologic - genetics ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism ; Infarction, Middle Cerebral Artery - complications ; ischemia ; Isocitrate Dehydrogenase - metabolism ; Male ; MAP Kinase Kinase 1 - metabolism ; Mass Spectrometry ; Polymerase Chain Reaction ; Proteins - isolation & purification ; Proteins - metabolism ; proteomics ; Proteomics - methods ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Journal of Veterinary Medical Science, 2012, Vol.74(11), pp.1401-1407</ispartof><rights>2012 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c635t-e08b0a07571dac868807c8c441d75c148b37b193ef5653e16fc0f3b34018877f3</citedby><cites>FETCH-LOGICAL-c635t-e08b0a07571dac868807c8c441d75c148b37b193ef5653e16fc0f3b34018877f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22785056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SUNG, Jin-Hee</creatorcontrib><creatorcontrib>CHO, Eun-Hae</creatorcontrib><creatorcontrib>CHO, Jae-Hyeon</creatorcontrib><creatorcontrib>WON, Chung-Kil</creatorcontrib><creatorcontrib>KIM, Myeong-Ok</creatorcontrib><creatorcontrib>KOH, Phil-Ok</creatorcontrib><title>Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD+], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes.</description><subject>Adenosylhomocysteinase - metabolism</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Brain - metabolism</subject><subject>Brain Ischemia - drug therapy</subject><subject>Brain Ischemia - etiology</subject><subject>Brain Ischemia - metabolism</subject><subject>Coumaric Acids - pharmacology</subject><subject>DNA Primers - genetics</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>ferulic acid</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Enzymologic - genetics</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism</subject><subject>Infarction, Middle Cerebral Artery - complications</subject><subject>ischemia</subject><subject>Isocitrate Dehydrogenase - metabolism</subject><subject>Male</subject><subject>MAP Kinase Kinase 1 - metabolism</subject><subject>Mass Spectrometry</subject><subject>Polymerase Chain Reaction</subject><subject>Proteins - isolation & purification</subject><subject>Proteins - metabolism</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2P1CAYh4nRuLOjN8-GxIsHq3y0hR6bietusps1Rs-EwttdJrSMQE3m4P8uY9c5CAm8gScPHz-E3lDykbKOfdr_mlKpKkJa_gxtKK9FJWrePUcb0tG2EqwhF-gypT0hjNZt9xJdMCZkQ5p2g37fWJizG53R2YUZhxF_jSGDmxP-Bg-L1xksHo74CuLincG9cRa7GWt856z1gHcQYYja4z5miEd8b4xf0snVz24q63fBgq_61RsmZxLuD4cYtHl8hV6M2id4_TRv0Y-rz99319Xt_ZebXX9bmZY3uQIiB6KJaAS12shWSiKMNHVNrWgMreXAxUA7DmPTNhxoOxoy8oHXhEopxMi36P3qLcf-XCBlNblkwHs9Q1iSoqw0zlnpW_TuP3QfljiX26nyeVIwTpgo1IeVMjGkFGFUh1geG4-KEnWKRZ1iKZU6xVLwt0_SZZjAnuF_ORRgtwL7lPUDnAEdszMeVpuoFaV_x1V73jWPOiqY-R_G6J_o</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>SUNG, Jin-Hee</creator><creator>CHO, Eun-Hae</creator><creator>CHO, Jae-Hyeon</creator><creator>WON, Chung-Kil</creator><creator>KIM, Myeong-Ok</creator><creator>KOH, Phil-Ok</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2012</creationdate><title>Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach</title><author>SUNG, Jin-Hee ; CHO, Eun-Hae ; CHO, Jae-Hyeon ; WON, Chung-Kil ; KIM, Myeong-Ok ; KOH, Phil-Ok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c635t-e08b0a07571dac868807c8c441d75c148b37b193ef5653e16fc0f3b34018877f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenosylhomocysteinase - metabolism</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Brain - metabolism</topic><topic>Brain Ischemia - drug therapy</topic><topic>Brain Ischemia - etiology</topic><topic>Brain Ischemia - metabolism</topic><topic>Coumaric Acids - pharmacology</topic><topic>DNA Primers - genetics</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>ferulic acid</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Enzymologic - genetics</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism</topic><topic>Infarction, Middle Cerebral Artery - complications</topic><topic>ischemia</topic><topic>Isocitrate Dehydrogenase - metabolism</topic><topic>Male</topic><topic>MAP Kinase Kinase 1 - metabolism</topic><topic>Mass Spectrometry</topic><topic>Polymerase Chain Reaction</topic><topic>Proteins - isolation & purification</topic><topic>Proteins - metabolism</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SUNG, Jin-Hee</creatorcontrib><creatorcontrib>CHO, Eun-Hae</creatorcontrib><creatorcontrib>CHO, Jae-Hyeon</creatorcontrib><creatorcontrib>WON, Chung-Kil</creatorcontrib><creatorcontrib>KIM, Myeong-Ok</creatorcontrib><creatorcontrib>KOH, Phil-Ok</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SUNG, Jin-Hee</au><au>CHO, Eun-Hae</au><au>CHO, Jae-Hyeon</au><au>WON, Chung-Kil</au><au>KIM, Myeong-Ok</au><au>KOH, Phil-Ok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2012</date><risdate>2012</risdate><volume>74</volume><issue>11</issue><spage>1401</spage><epage>1407</epage><pages>1401-1407</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD+], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>22785056</pmid><doi>10.1292/jvms.12-0063</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2012, Vol.74(11), pp.1401-1407 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1222233232 |
| source | J-STAGE (Free - Japanese); MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adenosylhomocysteinase - metabolism Animals Blotting, Western Brain - metabolism Brain Ischemia - drug therapy Brain Ischemia - etiology Brain Ischemia - metabolism Coumaric Acids - pharmacology DNA Primers - genetics Electrophoresis, Gel, Two-Dimensional ferulic acid Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Enzymologic - genetics Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism Infarction, Middle Cerebral Artery - complications ischemia Isocitrate Dehydrogenase - metabolism Male MAP Kinase Kinase 1 - metabolism Mass Spectrometry Polymerase Chain Reaction Proteins - isolation & purification Proteins - metabolism proteomics Proteomics - methods Rats Rats, Sprague-Dawley |
| title | Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T10%3A06%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20Proteins%20Regulated%20by%20Ferulic%20Acid%20in%20a%20Middle%20Cerebral%20Artery%20Occlusion%20Animal%20Model-A%20Proteomics%20Approach&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=SUNG,%20Jin-Hee&rft.date=2012&rft.volume=74&rft.issue=11&rft.spage=1401&rft.epage=1407&rft.pages=1401-1407&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.12-0063&rft_dat=%3Cproquest_cross%3E1222233232%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468723027&rft_id=info:pmid/22785056&rfr_iscdi=true |