Design of double-walled carbon nanotubes for biomedical applications
Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWN...
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| Veröffentlicht in: | Nanotechnology 2012-09, Vol.23 (36), p.365102-365102 |
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| container_issue | 36 |
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| container_title | Nanotechnology |
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| creator | Neves, V Heister, E Costa, S Tîlmaciu, C Flahaut, E Soula, B Coley, H M McFadden, J Silva, S R P |
| description | Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT-siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). Uptake of the custom-designed nanotubes was not associated with detectable biochemical perturbations in cultured cells, but transfection of cells with DWNTs loaded with siRNA targeting the green fluorescent protein (GFP) gene, serving as a model system, as well as with therapeutic siRNA targeting the survivin gene, led to a significant gene silencing effect, and in the latter case a resulting apoptotic effect in cancer cells. |
| doi_str_mv | 10.1088/0957-4484/23/36/365102 |
| format | Article |
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DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT-siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). Uptake of the custom-designed nanotubes was not associated with detectable biochemical perturbations in cultured cells, but transfection of cells with DWNTs loaded with siRNA targeting the green fluorescent protein (GFP) gene, serving as a model system, as well as with therapeutic siRNA targeting the survivin gene, led to a significant gene silencing effect, and in the latter case a resulting apoptotic effect in cancer cells.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/0957-4484/23/36/365102</identifier><identifier>PMID: 22914449</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Apoptosis ; Biochemistry, Molecular Biology ; Bioengineering ; Biomedical Research - methods ; Carbon nanotubes ; Cell Line, Tumor ; Chemical vapor deposition ; Crosslinking ; Engineering Sciences ; Flow Cytometry ; Gene Silencing ; Genes ; Green Fluorescent Proteins - metabolism ; Humans ; Life Sciences ; Micro and nanotechnologies ; Microelectronics ; Nanostructure ; Nanotechnology ; Nanotubes, Carbon - chemistry ; Polypeptides ; Ribonucleic acids ; RNA, Small Interfering - metabolism ; Spectrum Analysis, Raman ; Time Factors</subject><ispartof>Nanotechnology, 2012-09, Vol.23 (36), p.365102-365102</ispartof><rights>2012 IOP Publishing Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-3054fb2b6efee97994c74c817798bf5a6a9c86956bd897917462065756299c73</citedby><orcidid>0000-0001-8344-6902</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0957-4484/23/36/365102/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>230,314,776,780,881,27901,27902,53821,53868</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22914449$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-00858588$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Neves, V</creatorcontrib><creatorcontrib>Heister, E</creatorcontrib><creatorcontrib>Costa, S</creatorcontrib><creatorcontrib>Tîlmaciu, C</creatorcontrib><creatorcontrib>Flahaut, E</creatorcontrib><creatorcontrib>Soula, B</creatorcontrib><creatorcontrib>Coley, H M</creatorcontrib><creatorcontrib>McFadden, J</creatorcontrib><creatorcontrib>Silva, S R P</creatorcontrib><title>Design of double-walled carbon nanotubes for biomedical applications</title><title>Nanotechnology</title><addtitle>Nano</addtitle><addtitle>Nanotechnology</addtitle><description>Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT-siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). 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| subjects | Apoptosis Biochemistry, Molecular Biology Bioengineering Biomedical Research - methods Carbon nanotubes Cell Line, Tumor Chemical vapor deposition Crosslinking Engineering Sciences Flow Cytometry Gene Silencing Genes Green Fluorescent Proteins - metabolism Humans Life Sciences Micro and nanotechnologies Microelectronics Nanostructure Nanotechnology Nanotubes, Carbon - chemistry Polypeptides Ribonucleic acids RNA, Small Interfering - metabolism Spectrum Analysis, Raman Time Factors |
| title | Design of double-walled carbon nanotubes for biomedical applications |
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