Diagnosis of Kyasanur forest disease by nested RT-PCR, real-time RT-PCR and IgM capture ELISA

► Sensitive Taqman based qRT-PCR, nested RT-PCR and MAC ELISA were developed for rapid and accurate diagnosis of suspected Kyasanur Forest Disease (KFD) cases in India. ► Clinical samples and different KFD virus strains were screened using real-time RT-PCR and nRT-PCR and were found that real-time R...

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Veröffentlicht in:	Journal of virological methods 2012-12, Vol.186 (1-2), p.49-54
Hauptverfasser:	Mourya, Devendra T., Yadav, Pragya D., Mehla, Rajeev, Barde, Pradip V., Yergolkar, Prasanna N., Kumar, Sandeep R.P., Thakare, Jyotsna P., Mishra, Akhilesh C.
Format:	Artikel
Sprache:	eng
Schlagworte:	antibodies Antibodies, Viral - blood Biological and medical sciences blood serum Complement fixation complement fixation tests control methods Diagnosis diagnostic techniques early diagnosis Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Flaviviridae Flavivirus Flavivirus - genetics Flavivirus - immunology Forests Fundamental and applied biological sciences. Psychology hemagglutination human resources Humans Immunoglobulin M Immunoglobulin M - blood India Infection Kyasanur forest disease Kyasanur Forest Disease - diagnosis Kyasanur Forest disease virus MAC-ELISA Microbiology Nested RT-PCR nucleotide sequences Personnel Polymerase chain reaction Polymerase Chain Reaction - methods Public health Real-Time Polymerase Chain Reaction - methods reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RNA Sensitivity and Specificity Seroconversion Serological tests TaqMan real-time RT-PCR Techniques used in virology viral load Virology viruses
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