Impact of Ty3/Gypsy group retrotransposon Lila on the D-Genome specificity of common wheat Triticum aestivum L
An analysis of the primary structure of BAC clone 112D20 T. aestivum , that contains D-genome specific Ty3- Gypsy -retrotransposon Lila is presented. PCR analysis of nulli-tetrasomic and deletion lines of T. aestivum allowed to localize this BAC clone in the distal region of the long arm of chromoso...
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| Veröffentlicht in: | Molecular biology (New York) 2012-07, Vol.46 (4), p.522-530 |
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| creator | Shcherban’, A. B. Adonina, I. G. Salina, E. A. |
| description | An analysis of the primary structure of BAC clone 112D20
T. aestivum
, that contains D-genome specific Ty3-
Gypsy
-retrotransposon
Lila
is presented. PCR analysis of nulli-tetrasomic and deletion lines of
T. aestivum
allowed to localize this BAC clone in the distal region of the long arm of chromosome 5D. Characteristic feature of BAC clone 112D20 is a high concentration of Ty3-
Gypsy
-retrotransposons (61.7%), and low content of the genes (1.2%). Only a single open reading frame was revealed homologous to an unknown gene of
Ae. tauschii
. Specific to the D-genome Ty3-
Gypsy
-retrotransposon
Lila
in the BAC clone 112D20 is 14 kb in length and contains unequal in size long terminal repeats. The data of in situ hybridization and PCR analysis of different Triticeae species suggest that this retroelement was amplified within the ancestral species of
Ae. tauschii
, the donor D-genome. The suggested time of amplification based on estimation of insertion time of
Lila
112D20 is 1.7 million years, which corresponds to the formation of the first allopolyploid forms of wheat. Based on comparison with the previously obtained data, it is concluded that the amplification of retroelements specific to each genome of wheat took place during formation of the diploid progenitors of these genomes. |
| doi_str_mv | 10.1134/S002689331202015X |
| format | Article |
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T. aestivum
, that contains D-genome specific Ty3-
Gypsy
-retrotransposon
Lila
is presented. PCR analysis of nulli-tetrasomic and deletion lines of
T. aestivum
allowed to localize this BAC clone in the distal region of the long arm of chromosome 5D. Characteristic feature of BAC clone 112D20 is a high concentration of Ty3-
Gypsy
-retrotransposons (61.7%), and low content of the genes (1.2%). Only a single open reading frame was revealed homologous to an unknown gene of
Ae. tauschii
. Specific to the D-genome Ty3-
Gypsy
-retrotransposon
Lila
in the BAC clone 112D20 is 14 kb in length and contains unequal in size long terminal repeats. The data of in situ hybridization and PCR analysis of different Triticeae species suggest that this retroelement was amplified within the ancestral species of
Ae. tauschii
, the donor D-genome. The suggested time of amplification based on estimation of insertion time of
Lila
112D20 is 1.7 million years, which corresponds to the formation of the first allopolyploid forms of wheat. Based on comparison with the previously obtained data, it is concluded that the amplification of retroelements specific to each genome of wheat took place during formation of the diploid progenitors of these genomes.</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1134/S002689331202015X</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Amino acid sequence ; Bacterial artificial chromosomes ; Biochemistry ; Biomedical and Life Sciences ; Chromosome deletion ; Chromosomes ; Clonal deletion ; Cloning ; Data processing ; Diploids ; Genomes ; Genomics ; Genomics. Transcriptomics ; Human Genetics ; Insertion ; Life Sciences ; Long terminal repeat ; Molecular biology ; Open reading frames ; Polymerase chain reaction ; Retrotransposons ; Stem cells ; Triticeae ; Triticum aestivum ; Wheat</subject><ispartof>Molecular biology (New York), 2012-07, Vol.46 (4), p.522-530</ispartof><rights>Pleiades Publishing, Ltd. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-610688b63fe89faf6cb042bbf4911ec05f32a01555580f53453f519a959e20e23</citedby><cites>FETCH-LOGICAL-c349t-610688b63fe89faf6cb042bbf4911ec05f32a01555580f53453f519a959e20e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S002689331202015X$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S002689331202015X$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Shcherban’, A. B.</creatorcontrib><creatorcontrib>Adonina, I. G.</creatorcontrib><creatorcontrib>Salina, E. A.</creatorcontrib><title>Impact of Ty3/Gypsy group retrotransposon Lila on the D-Genome specificity of common wheat Triticum aestivum L</title><title>Molecular biology (New York)</title><addtitle>Mol Biol</addtitle><description>An analysis of the primary structure of BAC clone 112D20
T. aestivum
, that contains D-genome specific Ty3-
Gypsy
-retrotransposon
Lila
is presented. PCR analysis of nulli-tetrasomic and deletion lines of
T. aestivum
allowed to localize this BAC clone in the distal region of the long arm of chromosome 5D. Characteristic feature of BAC clone 112D20 is a high concentration of Ty3-
Gypsy
-retrotransposons (61.7%), and low content of the genes (1.2%). Only a single open reading frame was revealed homologous to an unknown gene of
Ae. tauschii
. Specific to the D-genome Ty3-
Gypsy
-retrotransposon
Lila
in the BAC clone 112D20 is 14 kb in length and contains unequal in size long terminal repeats. The data of in situ hybridization and PCR analysis of different Triticeae species suggest that this retroelement was amplified within the ancestral species of
Ae. tauschii
, the donor D-genome. The suggested time of amplification based on estimation of insertion time of
Lila
112D20 is 1.7 million years, which corresponds to the formation of the first allopolyploid forms of wheat. Based on comparison with the previously obtained data, it is concluded that the amplification of retroelements specific to each genome of wheat took place during formation of the diploid progenitors of these genomes.</description><subject>Amino acid sequence</subject><subject>Bacterial artificial chromosomes</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Chromosome deletion</subject><subject>Chromosomes</subject><subject>Clonal deletion</subject><subject>Cloning</subject><subject>Data processing</subject><subject>Diploids</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genomics. Transcriptomics</subject><subject>Human Genetics</subject><subject>Insertion</subject><subject>Life Sciences</subject><subject>Long terminal repeat</subject><subject>Molecular biology</subject><subject>Open reading frames</subject><subject>Polymerase chain reaction</subject><subject>Retrotransposons</subject><subject>Stem cells</subject><subject>Triticeae</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFKxDAQhoMouK4-gLeAFy91M0lamqOsugoFD67graQh2c2ybWqSKn17U9aDKIaBCcz3_5PJIHQJ5AaA8cULIbQoBWNACSWQvx2hGRSkzBjl-TGaTeVsqp-isxB2hEAKOkPdU9tLFbEzeD2yxWrsw4g33g099jp6F73sQu-C63Bl9xKnHLca32Ur3blW49BrZY1VNo6Th3Jtm5DPrZYRr72NVg0tljpE-5Eu1Tk6MXIf9MV3nqPXh_v18jGrnldPy9sqU4yLmBVAirJsCmZ0KYw0hWoIp01juADQiuSGUZmmTKckJmc8ZyYHIUUuNCWasjm6Pvj23r0PqX3d2qD0fi877YZQA6UAnIiSJPTqF7pzg-_S62ogTIDg6acSBQdKeReC16buvW2lHxNUTxuo_2wgaehBExLbbbT_6fyf6AtMo4bl</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Shcherban’, A. B.</creator><creator>Adonina, I. G.</creator><creator>Salina, E. A.</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20120701</creationdate><title>Impact of Ty3/Gypsy group retrotransposon Lila on the D-Genome specificity of common wheat Triticum aestivum L</title><author>Shcherban’, A. B. ; Adonina, I. G. ; Salina, E. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-610688b63fe89faf6cb042bbf4911ec05f32a01555580f53453f519a959e20e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino acid sequence</topic><topic>Bacterial artificial chromosomes</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Chromosome deletion</topic><topic>Chromosomes</topic><topic>Clonal deletion</topic><topic>Cloning</topic><topic>Data processing</topic><topic>Diploids</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Genomics. Transcriptomics</topic><topic>Human Genetics</topic><topic>Insertion</topic><topic>Life Sciences</topic><topic>Long terminal repeat</topic><topic>Molecular biology</topic><topic>Open reading frames</topic><topic>Polymerase chain reaction</topic><topic>Retrotransposons</topic><topic>Stem cells</topic><topic>Triticeae</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shcherban’, A. B.</creatorcontrib><creatorcontrib>Adonina, I. G.</creatorcontrib><creatorcontrib>Salina, E. 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B.</au><au>Adonina, I. G.</au><au>Salina, E. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of Ty3/Gypsy group retrotransposon Lila on the D-Genome specificity of common wheat Triticum aestivum L</atitle><jtitle>Molecular biology (New York)</jtitle><stitle>Mol Biol</stitle><date>2012-07-01</date><risdate>2012</risdate><volume>46</volume><issue>4</issue><spage>522</spage><epage>530</epage><pages>522-530</pages><issn>0026-8933</issn><eissn>1608-3245</eissn><abstract>An analysis of the primary structure of BAC clone 112D20
T. aestivum
, that contains D-genome specific Ty3-
Gypsy
-retrotransposon
Lila
is presented. PCR analysis of nulli-tetrasomic and deletion lines of
T. aestivum
allowed to localize this BAC clone in the distal region of the long arm of chromosome 5D. Characteristic feature of BAC clone 112D20 is a high concentration of Ty3-
Gypsy
-retrotransposons (61.7%), and low content of the genes (1.2%). Only a single open reading frame was revealed homologous to an unknown gene of
Ae. tauschii
. Specific to the D-genome Ty3-
Gypsy
-retrotransposon
Lila
in the BAC clone 112D20 is 14 kb in length and contains unequal in size long terminal repeats. The data of in situ hybridization and PCR analysis of different Triticeae species suggest that this retroelement was amplified within the ancestral species of
Ae. tauschii
, the donor D-genome. The suggested time of amplification based on estimation of insertion time of
Lila
112D20 is 1.7 million years, which corresponds to the formation of the first allopolyploid forms of wheat. Based on comparison with the previously obtained data, it is concluded that the amplification of retroelements specific to each genome of wheat took place during formation of the diploid progenitors of these genomes.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S002689331202015X</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-8933 |
| ispartof | Molecular biology (New York), 2012-07, Vol.46 (4), p.522-530 |
| issn | 0026-8933 1608-3245 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1221140980 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Amino acid sequence Bacterial artificial chromosomes Biochemistry Biomedical and Life Sciences Chromosome deletion Chromosomes Clonal deletion Cloning Data processing Diploids Genomes Genomics Genomics. Transcriptomics Human Genetics Insertion Life Sciences Long terminal repeat Molecular biology Open reading frames Polymerase chain reaction Retrotransposons Stem cells Triticeae Triticum aestivum Wheat |
| title | Impact of Ty3/Gypsy group retrotransposon Lila on the D-Genome specificity of common wheat Triticum aestivum L |
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