Cell culture assay system for the evaluation of natural product-mediated anti-Hepatitis B virus activity
Utilizing the hepatitis B virus (HBV)-producing hepatoblastoma cell line, HepG2.2.15, which is stably transfected with the cloned HBV genome, methods were devised to examine the effects of test substances on intracellular extrachromosomal HBV DNA levels and secretion of hepatitis B surface antigen (...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 1997-02, Vol.3 (4), p.369-377 |
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| creator | Mehdi, H. Tan, G.T. Pezzuto, J.M. Fong, H.H.S. Farnsworth, N.R. El-Feraly, F.S. Al-Yahya, M.A. Mossa, J.S. Peeples, M.E. Kernan, M.R. Rozhon, E.J. |
| description | Utilizing the hepatitis B virus (HBV)-producing hepatoblastoma cell line, HepG2.2.15, which is stably transfected with the cloned HBV genome, methods were devised to examine the effects of test substances on intracellular extrachromosomal HBV DNA levels and secretion of hepatitis B surface antigen (HBsAg). The known inhibitor of HBV replication, dideoxycytosine (ddC), had a minor effect on the secretion of HBsAg, but >90% of intracellular extrachromosomal HBV DNA expression was lost at a non-cytotoxic drug concentration (25μM). This inhibitory effect was reversed when ddC was removed from the medium. Of 19 plant materials tested, extracts from the aerial parts of
Clematis sinensis Lour, and
Clerodendron inerme R. Br. significantly inhibited the secretion of HBsAg into the culture medium at non-cytotoxic concentrations, but had no effect on intracellular extrachromosomal HBV DNA levels. This system is useful for the evaluation of test materials, or combinations of test materials, for their potential to inhibit HBV markers. |
| doi_str_mv | 10.1016/S0944-7113(97)80011-6 |
| format | Article |
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Clematis sinensis Lour, and
Clerodendron inerme R. Br. significantly inhibited the secretion of HBsAg into the culture medium at non-cytotoxic concentrations, but had no effect on intracellular extrachromosomal HBV DNA levels. This system is useful for the evaluation of test materials, or combinations of test materials, for their potential to inhibit HBV markers.</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/S0944-7113(97)80011-6</identifier><identifier>PMID: 23195196</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>antiviral ; ccc (covalently closed circular) DNA ; cytotoxicity ; HBsAg ; Hepatitis B virus ; plant extracts ; rc (relaxed circular) DNA</subject><ispartof>Phytomedicine (Stuttgart), 1997-02, Vol.3 (4), p.369-377</ispartof><rights>1996 Gustav Fischer Verlag</rights><rights>Copyright © 1996 Gustav Fischer Verlag. Published by Elsevier GmbH.. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-a3fd96aeed16398be0b061d437e3bb00372d1cefd0594e1fc126a7838cb1709d3</citedby><cites>FETCH-LOGICAL-c295t-a3fd96aeed16398be0b061d437e3bb00372d1cefd0594e1fc126a7838cb1709d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0944-7113(97)80011-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23195196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehdi, H.</creatorcontrib><creatorcontrib>Tan, G.T.</creatorcontrib><creatorcontrib>Pezzuto, J.M.</creatorcontrib><creatorcontrib>Fong, H.H.S.</creatorcontrib><creatorcontrib>Farnsworth, N.R.</creatorcontrib><creatorcontrib>El-Feraly, F.S.</creatorcontrib><creatorcontrib>Al-Yahya, M.A.</creatorcontrib><creatorcontrib>Mossa, J.S.</creatorcontrib><creatorcontrib>Peeples, M.E.</creatorcontrib><creatorcontrib>Kernan, M.R.</creatorcontrib><creatorcontrib>Rozhon, E.J.</creatorcontrib><title>Cell culture assay system for the evaluation of natural product-mediated anti-Hepatitis B virus activity</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Utilizing the hepatitis B virus (HBV)-producing hepatoblastoma cell line, HepG2.2.15, which is stably transfected with the cloned HBV genome, methods were devised to examine the effects of test substances on intracellular extrachromosomal HBV DNA levels and secretion of hepatitis B surface antigen (HBsAg). The known inhibitor of HBV replication, dideoxycytosine (ddC), had a minor effect on the secretion of HBsAg, but >90% of intracellular extrachromosomal HBV DNA expression was lost at a non-cytotoxic drug concentration (25μM). This inhibitory effect was reversed when ddC was removed from the medium. Of 19 plant materials tested, extracts from the aerial parts of
Clematis sinensis Lour, and
Clerodendron inerme R. Br. significantly inhibited the secretion of HBsAg into the culture medium at non-cytotoxic concentrations, but had no effect on intracellular extrachromosomal HBV DNA levels. This system is useful for the evaluation of test materials, or combinations of test materials, for their potential to inhibit HBV markers.</description><subject>antiviral</subject><subject>ccc (covalently closed circular) DNA</subject><subject>cytotoxicity</subject><subject>HBsAg</subject><subject>Hepatitis B virus</subject><subject>plant extracts</subject><subject>rc (relaxed circular) DNA</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkMtOwzAQRS0EgvL4BJCXsAh44sSJVwgqXhISC0BiZzn2RDVKk2I7lfr3uC2wZTWbM3PnHkJOgV0CA3H1ymRRZBUAP5fVRc0YQCZ2yAQE1BmT5ccumfwhB-QwhM_EFLJi--Qg5yBLkGJCZlPsOmrGLo4eqQ5Br2hYhYhz2g6exhlSXOpu1NENPR1a2utE6o4u_GBHE7M5WqcjWqr76LJHXCQyukBv6dL5MVBtolu6uDome63uAp78zCPyfn_3Nn3Mnl8enqY3z5nJZRkzzVsrhUa0ILisG2QNE2ALXiFvGsZ4lVsw2FpWygKhNZALXdW8Ng1UTFp-RM63d9ODXyOGqOYumFRS9ziMQUGeJyFlIXhCyy1q_BCCx1YtvJtrv1LA1Fqy2khWa4NKVmojWYm0d_YTMTap_t_Wr9UEXG8BTEWXDr0KxmFvkiqPJio7uH8ivgHoRY2w</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Mehdi, H.</creator><creator>Tan, G.T.</creator><creator>Pezzuto, J.M.</creator><creator>Fong, H.H.S.</creator><creator>Farnsworth, N.R.</creator><creator>El-Feraly, F.S.</creator><creator>Al-Yahya, M.A.</creator><creator>Mossa, J.S.</creator><creator>Peeples, M.E.</creator><creator>Kernan, M.R.</creator><creator>Rozhon, E.J.</creator><general>Elsevier GmbH</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970201</creationdate><title>Cell culture assay system for the evaluation of natural product-mediated anti-Hepatitis B virus activity</title><author>Mehdi, H. ; Tan, G.T. ; Pezzuto, J.M. ; Fong, H.H.S. ; Farnsworth, N.R. ; El-Feraly, F.S. ; Al-Yahya, M.A. ; Mossa, J.S. ; Peeples, M.E. ; Kernan, M.R. ; Rozhon, E.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-a3fd96aeed16398be0b061d437e3bb00372d1cefd0594e1fc126a7838cb1709d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>antiviral</topic><topic>ccc (covalently closed circular) DNA</topic><topic>cytotoxicity</topic><topic>HBsAg</topic><topic>Hepatitis B virus</topic><topic>plant extracts</topic><topic>rc (relaxed circular) DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehdi, H.</creatorcontrib><creatorcontrib>Tan, G.T.</creatorcontrib><creatorcontrib>Pezzuto, J.M.</creatorcontrib><creatorcontrib>Fong, H.H.S.</creatorcontrib><creatorcontrib>Farnsworth, N.R.</creatorcontrib><creatorcontrib>El-Feraly, F.S.</creatorcontrib><creatorcontrib>Al-Yahya, M.A.</creatorcontrib><creatorcontrib>Mossa, J.S.</creatorcontrib><creatorcontrib>Peeples, M.E.</creatorcontrib><creatorcontrib>Kernan, M.R.</creatorcontrib><creatorcontrib>Rozhon, E.J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehdi, H.</au><au>Tan, G.T.</au><au>Pezzuto, J.M.</au><au>Fong, H.H.S.</au><au>Farnsworth, N.R.</au><au>El-Feraly, F.S.</au><au>Al-Yahya, M.A.</au><au>Mossa, J.S.</au><au>Peeples, M.E.</au><au>Kernan, M.R.</au><au>Rozhon, E.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell culture assay system for the evaluation of natural product-mediated anti-Hepatitis B virus activity</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>3</volume><issue>4</issue><spage>369</spage><epage>377</epage><pages>369-377</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Utilizing the hepatitis B virus (HBV)-producing hepatoblastoma cell line, HepG2.2.15, which is stably transfected with the cloned HBV genome, methods were devised to examine the effects of test substances on intracellular extrachromosomal HBV DNA levels and secretion of hepatitis B surface antigen (HBsAg). The known inhibitor of HBV replication, dideoxycytosine (ddC), had a minor effect on the secretion of HBsAg, but >90% of intracellular extrachromosomal HBV DNA expression was lost at a non-cytotoxic drug concentration (25μM). This inhibitory effect was reversed when ddC was removed from the medium. Of 19 plant materials tested, extracts from the aerial parts of
Clematis sinensis Lour, and
Clerodendron inerme R. Br. significantly inhibited the secretion of HBsAg into the culture medium at non-cytotoxic concentrations, but had no effect on intracellular extrachromosomal HBV DNA levels. This system is useful for the evaluation of test materials, or combinations of test materials, for their potential to inhibit HBV markers.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>23195196</pmid><doi>10.1016/S0944-7113(97)80011-6</doi><tpages>9</tpages></addata></record> |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | antiviral ccc (covalently closed circular) DNA cytotoxicity HBsAg Hepatitis B virus plant extracts rc (relaxed circular) DNA |
| title | Cell culture assay system for the evaluation of natural product-mediated anti-Hepatitis B virus activity |
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