Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells
Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtype...
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| Veröffentlicht in: | Endocrine 2012-12, Vol.42 (3), p.670-675 |
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| creator | Su, Zhipeng Jiang, Xiaolong Wang, Chengde Liu, Jie Chen, Yunxiang Li, Qun Wu, Jinsen Zheng, Weiming Zhuge, Qichuan Jin, Kunlin Wu, Zhebao |
| description | Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L. |
| doi_str_mv | 10.1007/s12020-012-9715-y |
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As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.</description><identifier>ISSN: 1355-008X</identifier><identifier>EISSN: 1559-0100</identifier><identifier>DOI: 10.1007/s12020-012-9715-y</identifier><identifier>PMID: 22684586</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Apoptosis - drug effects ; Bromocriptine - pharmacology ; Cell Line, Tumor ; Cell Survival - drug effects ; Diabetes ; Endocrinology ; Flow Cytometry ; Humanities and Social Sciences ; Internal Medicine ; Medicine ; Medicine & Public Health ; multidisciplinary ; Nerve Growth Factor - pharmacology ; Original Article ; Pituitary Neoplasms - metabolism ; Rats ; Real-Time Polymerase Chain Reaction ; Receptors, Dopamine D2 - agonists ; Receptors, Dopamine D2 - biosynthesis ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Science</subject><ispartof>Endocrine, 2012-12, Vol.42 (3), p.670-675</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-31951080a97fdbee6bce5478089bac43605a7fbfe1d9f67c4fda6168528b859f3</citedby><cites>FETCH-LOGICAL-c344t-31951080a97fdbee6bce5478089bac43605a7fbfe1d9f67c4fda6168528b859f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12020-012-9715-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12020-012-9715-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22684586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Zhipeng</creatorcontrib><creatorcontrib>Jiang, Xiaolong</creatorcontrib><creatorcontrib>Wang, Chengde</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Chen, Yunxiang</creatorcontrib><creatorcontrib>Li, Qun</creatorcontrib><creatorcontrib>Wu, Jinsen</creatorcontrib><creatorcontrib>Zheng, Weiming</creatorcontrib><creatorcontrib>Zhuge, Qichuan</creatorcontrib><creatorcontrib>Jin, Kunlin</creatorcontrib><creatorcontrib>Wu, Zhebao</creatorcontrib><title>Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells</title><title>Endocrine</title><addtitle>Endocrine</addtitle><addtitle>Endocrine</addtitle><description>Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Bromocriptine - pharmacology</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Diabetes</subject><subject>Endocrinology</subject><subject>Flow Cytometry</subject><subject>Humanities and Social Sciences</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>multidisciplinary</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>Original Article</subject><subject>Pituitary Neoplasms - metabolism</subject><subject>Rats</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Dopamine D2 - agonists</subject><subject>Receptors, Dopamine D2 - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Science</subject><issn>1355-008X</issn><issn>1559-0100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3TAQha0KVP76AGyQl2wCYydO7CUCCkhI3RSJneU4Y2p0EwfbKdw-Pb66lCWrOZr55mjmEHLM4IwBdOeJceBQAeOV6pio1t_IPhNClQ7ATtG1EBWAfNwjByk9A3DO2-472StFNkK2--TflXcOI07ZmxXFom1ONDg6YfyL9CmG1_yHOmNziDRMFN_miCn5Igs0hNmMfkLKaUSL8wZKS5_XMybqJ3pzW9NoMp19Xnw2cU3zMhbG4mqVjsiuM6uEPz7qIXn4ef378ra6_3Vzd3lxX9m6aXJVMyUYSDCqc0OP2PYWRdNJkKo3tqlbEKZzvUM2KNd2tnGDaVkrBZe9FMrVh-R06zvH8LJgynr0aXOBmTAsSTPOGeNKtaygbIvaGFKK6PQc_Vju1gz0JnK9jVyXyPUmcr0uOycf9ks_4vC58T_jAvAtkMpoesKon8MSp_LyF67vl8yOnw</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Su, Zhipeng</creator><creator>Jiang, Xiaolong</creator><creator>Wang, Chengde</creator><creator>Liu, Jie</creator><creator>Chen, Yunxiang</creator><creator>Li, Qun</creator><creator>Wu, Jinsen</creator><creator>Zheng, Weiming</creator><creator>Zhuge, Qichuan</creator><creator>Jin, Kunlin</creator><creator>Wu, Zhebao</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121201</creationdate><title>Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells</title><author>Su, Zhipeng ; Jiang, Xiaolong ; Wang, Chengde ; Liu, Jie ; Chen, Yunxiang ; Li, Qun ; Wu, Jinsen ; Zheng, Weiming ; Zhuge, Qichuan ; Jin, Kunlin ; Wu, Zhebao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-31951080a97fdbee6bce5478089bac43605a7fbfe1d9f67c4fda6168528b859f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Bromocriptine - pharmacology</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Diabetes</topic><topic>Endocrinology</topic><topic>Flow Cytometry</topic><topic>Humanities and Social Sciences</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>multidisciplinary</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>Original Article</topic><topic>Pituitary Neoplasms - metabolism</topic><topic>Rats</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Dopamine D2 - agonists</topic><topic>Receptors, Dopamine D2 - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Zhipeng</creatorcontrib><creatorcontrib>Jiang, Xiaolong</creatorcontrib><creatorcontrib>Wang, Chengde</creatorcontrib><creatorcontrib>Liu, Jie</creatorcontrib><creatorcontrib>Chen, Yunxiang</creatorcontrib><creatorcontrib>Li, Qun</creatorcontrib><creatorcontrib>Wu, Jinsen</creatorcontrib><creatorcontrib>Zheng, Weiming</creatorcontrib><creatorcontrib>Zhuge, Qichuan</creatorcontrib><creatorcontrib>Jin, Kunlin</creatorcontrib><creatorcontrib>Wu, Zhebao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Zhipeng</au><au>Jiang, Xiaolong</au><au>Wang, Chengde</au><au>Liu, Jie</au><au>Chen, Yunxiang</au><au>Li, Qun</au><au>Wu, Jinsen</au><au>Zheng, Weiming</au><au>Zhuge, Qichuan</au><au>Jin, Kunlin</au><au>Wu, Zhebao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells</atitle><jtitle>Endocrine</jtitle><stitle>Endocrine</stitle><addtitle>Endocrine</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>42</volume><issue>3</issue><spage>670</spage><epage>675</epage><pages>670-675</pages><issn>1355-008X</issn><eissn>1559-0100</eissn><abstract>Nerve growth factor (NGF) can increase expression of dopamine 2 receptors (D2R) in GH3 rat pituitary tumor cells (GH3 cells). As D2R exists in long (D2L) and short (D2S) isoforms, effects of NGF on D2R subtypes have not been accurately evaluated. In this study, we compared mRNA levels of D2R subtypes in GH3 cells treated with or without NGF with real-time RT-PCR. In addition, we also evaluated the relationship between GH3 cell growth after bromocriptine treatment and mRNA levels of D2R subtypes. We found that D2R total, D2L, and D2S mRNA in GH3 cells were significantly increased after NGF treatment, compared with the vehicle group. Moreover, NGF increased the ratio of D2S to D2L. GH3 cell survival rate after bromocriptine treatment was negatively correlated with D2R total mRNA, and D2S may be more potent than D2L in inhibiting cell growth. Cell apoptosis rate was highly elevated in GH3 cells treated with NGF and bromocriptine, compared with the control group or the group treated with NGF or bromocriptine alone. Our data provide preliminary evidence that the effect of NGF was more prominent on expression of D2S than D2L, in addition, D2S might have a greater impact suppressing GH3 cells growth than D2L.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22684586</pmid><doi>10.1007/s12020-012-9715-y</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Bromocriptine - pharmacology Cell Line, Tumor Cell Survival - drug effects Diabetes Endocrinology Flow Cytometry Humanities and Social Sciences Internal Medicine Medicine Medicine & Public Health multidisciplinary Nerve Growth Factor - pharmacology Original Article Pituitary Neoplasms - metabolism Rats Real-Time Polymerase Chain Reaction Receptors, Dopamine D2 - agonists Receptors, Dopamine D2 - biosynthesis RNA, Messenger - biosynthesis RNA, Messenger - genetics Science |
| title | Differential effects of nerve growth factor on expression of dopamine 2 receptor subtypes in GH3 rat pituitary tumor cells |
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