Methylenetetrahydrofolate reductase gene polymorphisms (677C > T and 1298A > C) in Egyptian patients with non-Hodgkin lymphoma
Folate metabolism plays an essential role in Deoxyribonucleic acid (DNA) synthesis and methylation processes. Deviations in the flux of the folate may affect the susceptibility to various cancers including lymphoma. The aim of this study was to investigate the genetic polymorphisms in 5, 10-methylen...
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| Veröffentlicht in: | Journal of cancer research and therapeutics 2012-07, Vol.8 (3), p.355-360 |
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| description | Folate metabolism plays an essential role in Deoxyribonucleic acid (DNA) synthesis and methylation processes. Deviations in the flux of the folate may affect the susceptibility to various cancers including lymphoma.
The aim of this study was to investigate the genetic polymorphisms in 5, 10-methylenetetrahydrofolate reductase (MTHFR 677C/T and 1298A/C) and to evaluate its associations with the risk of Non Hodgkin lymphoma.
The study included 50 patients with diffuse large B cell lymphoma (DLBCL) as well as 50 age matched apparently healthy volunteers (as control). All the subjects included in the study were genotyped for the detection of the MTHFR gene polymorphisms (677C > T and 1298A > C) by using restriction fragment length polymorphism (PCR-RFLP).
There were highly statistically significant differences between the 2 groups with respect to results of PCR-RFLP for MTHFR 677C→T polymorphism for CC genotype (P value = 0.001), statistically significant differences for CT (P value = 0.048) and TT (P value = 0.038) genotypes; however, no statistically significant differences regarding CC/CT or TT/CT alleles (P value = 0.052). Also, there were highly statistically significant differences between the patient and control groups with regards to the results of MTHFR1298 A/C polymorphism for the AA, AC genotypes as well as the AA/AC and CC/AC alleles (P value < 0.0001), and statistically significant difference regarding CC genotype (P value 0.0192).
In conclusion, this study demonstrated a significant association between the MTHFR polymorphisms and the risk of DLBCL. Thus the study could support that folate intake together with the genetic basis may help in modifying the risk to lymphoma. |
| doi_str_mv | 10.4103/0973-1482.103512 |
| format | Article |
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The aim of this study was to investigate the genetic polymorphisms in 5, 10-methylenetetrahydrofolate reductase (MTHFR 677C/T and 1298A/C) and to evaluate its associations with the risk of Non Hodgkin lymphoma.
The study included 50 patients with diffuse large B cell lymphoma (DLBCL) as well as 50 age matched apparently healthy volunteers (as control). All the subjects included in the study were genotyped for the detection of the MTHFR gene polymorphisms (677C > T and 1298A > C) by using restriction fragment length polymorphism (PCR-RFLP).
There were highly statistically significant differences between the 2 groups with respect to results of PCR-RFLP for MTHFR 677C→T polymorphism for CC genotype (P value = 0.001), statistically significant differences for CT (P value = 0.048) and TT (P value = 0.038) genotypes; however, no statistically significant differences regarding CC/CT or TT/CT alleles (P value = 0.052). Also, there were highly statistically significant differences between the patient and control groups with regards to the results of MTHFR1298 A/C polymorphism for the AA, AC genotypes as well as the AA/AC and CC/AC alleles (P value < 0.0001), and statistically significant difference regarding CC genotype (P value 0.0192).
In conclusion, this study demonstrated a significant association between the MTHFR polymorphisms and the risk of DLBCL. Thus the study could support that folate intake together with the genetic basis may help in modifying the risk to lymphoma.</description><identifier>ISSN: 0973-1482</identifier><identifier>EISSN: 1998-4138</identifier><identifier>DOI: 10.4103/0973-1482.103512</identifier><identifier>PMID: 23174714</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Abdomen ; Adult ; Aged ; Cardiovascular disease ; Colorectal cancer ; Deoxyribonucleic acid ; Dihydrofolate reductase ; Disease susceptibility ; DNA ; DNA methylation ; Egypt ; Enzymes ; Female ; Folic Acid - metabolism ; Gene Frequency ; Genes ; Genetic aspects ; Genetic Association Studies ; Genetic Predisposition to Disease ; Genotype ; Haplotypes ; Humans ; Informed consent ; Lymphoma ; Lymphoma, Large B-Cell, Diffuse - genetics ; Lymphoma, Non-Hodgkin - genetics ; Male ; Methylenetetrahydrofolate Reductase (NADPH2) - genetics ; Middle Aged ; Non-Hodgkin's lymphomas ; Nuclear medicine ; Physiological aspects ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; Risk ; Risk factors ; Single nucleotide polymorphisms ; Studies ; Vitamin B ; Young Adult</subject><ispartof>Journal of cancer research and therapeutics, 2012-07, Vol.8 (3), p.355-360</ispartof><rights>COPYRIGHT 2012 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt Ltd Jul 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-edf5b2efb8d932616f407896268d7782ec0cebd11ec534edf2b321cd04b77313</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23174714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nasr, Aml S</creatorcontrib><creatorcontrib>Sami, Rania M</creatorcontrib><creatorcontrib>Ibrahim, Noha Y</creatorcontrib><title>Methylenetetrahydrofolate reductase gene polymorphisms (677C > T and 1298A > C) in Egyptian patients with non-Hodgkin lymphoma</title><title>Journal of cancer research and therapeutics</title><addtitle>J Cancer Res Ther</addtitle><description>Folate metabolism plays an essential role in Deoxyribonucleic acid (DNA) synthesis and methylation processes. Deviations in the flux of the folate may affect the susceptibility to various cancers including lymphoma.
The aim of this study was to investigate the genetic polymorphisms in 5, 10-methylenetetrahydrofolate reductase (MTHFR 677C/T and 1298A/C) and to evaluate its associations with the risk of Non Hodgkin lymphoma.
The study included 50 patients with diffuse large B cell lymphoma (DLBCL) as well as 50 age matched apparently healthy volunteers (as control). All the subjects included in the study were genotyped for the detection of the MTHFR gene polymorphisms (677C > T and 1298A > C) by using restriction fragment length polymorphism (PCR-RFLP).
There were highly statistically significant differences between the 2 groups with respect to results of PCR-RFLP for MTHFR 677C→T polymorphism for CC genotype (P value = 0.001), statistically significant differences for CT (P value = 0.048) and TT (P value = 0.038) genotypes; however, no statistically significant differences regarding CC/CT or TT/CT alleles (P value = 0.052). Also, there were highly statistically significant differences between the patient and control groups with regards to the results of MTHFR1298 A/C polymorphism for the AA, AC genotypes as well as the AA/AC and CC/AC alleles (P value < 0.0001), and statistically significant difference regarding CC genotype (P value 0.0192).
In conclusion, this study demonstrated a significant association between the MTHFR polymorphisms and the risk of DLBCL. Thus the study could support that folate intake together with the genetic basis may help in modifying the risk to lymphoma.</description><subject>Abdomen</subject><subject>Adult</subject><subject>Aged</subject><subject>Cardiovascular disease</subject><subject>Colorectal cancer</subject><subject>Deoxyribonucleic acid</subject><subject>Dihydrofolate reductase</subject><subject>Disease susceptibility</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Egypt</subject><subject>Enzymes</subject><subject>Female</subject><subject>Folic Acid - metabolism</subject><subject>Gene Frequency</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic Association Studies</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotype</subject><subject>Haplotypes</subject><subject>Humans</subject><subject>Informed consent</subject><subject>Lymphoma</subject><subject>Lymphoma, Large B-Cell, Diffuse - genetics</subject><subject>Lymphoma, Non-Hodgkin - genetics</subject><subject>Male</subject><subject>Methylenetetrahydrofolate Reductase (NADPH2) - genetics</subject><subject>Middle Aged</subject><subject>Non-Hodgkin's lymphomas</subject><subject>Nuclear medicine</subject><subject>Physiological aspects</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Risk</subject><subject>Risk factors</subject><subject>Single nucleotide polymorphisms</subject><subject>Studies</subject><subject>Vitamin B</subject><subject>Young Adult</subject><issn>0973-1482</issn><issn>1998-4138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptks1v1DAQxSMEotvCnROyxKUcsvgrsXNBWq1Ki1TEZe-WE082KYkdbEdVLvztONpSBFr5YI3n957Go5dl7wjecoLZJ1wJlhMu6TZVBaEvsg2pKplzwuTLbPPcvsguQ3jAuBCUytfZBWVEcEH4Jvv1DWK3DGAhQvS6W4x3rRt0BOTBzE3UAdAxtdHkhmV0fur6MAZ0XQqxR5_RAWlrEKGV3KVq_xH1Ft0clyn22qJJxx5sDOixjx2yzuZ3zhx_JCRZTZ0b9ZvsVauHAG-f7qvs8OXmsL_L77_fft3v7vOGCx5zMG1RU2hraSpGS1K2HAtZlbSURghJocEN1IYQaArGE01rRkljMK-FYIRdZdcn28m7nzOEqMY-NDAM2oKbgyKkElwWVYkT-uE_9MHN3qbhFKGc4grzSv6ljnoA1dvWpeU1q6naMUJLTouCJyo_Q63b9HpwFto-Pf_Db8_w6RgY--asAJ8EjXcheGjV5PtR-0URrNaEqDUCao2AOiUkSd4__W-uRzDPgj-RYL8BPHiypg</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Nasr, Aml S</creator><creator>Sami, Rania M</creator><creator>Ibrahim, Noha Y</creator><general>Medknow Publications and Media Pvt. 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metabolism</topic><topic>Gene Frequency</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic Association Studies</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotype</topic><topic>Haplotypes</topic><topic>Humans</topic><topic>Informed consent</topic><topic>Lymphoma</topic><topic>Lymphoma, Large B-Cell, Diffuse - genetics</topic><topic>Lymphoma, Non-Hodgkin - genetics</topic><topic>Male</topic><topic>Methylenetetrahydrofolate Reductase (NADPH2) - genetics</topic><topic>Middle Aged</topic><topic>Non-Hodgkin's lymphomas</topic><topic>Nuclear medicine</topic><topic>Physiological aspects</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Risk</topic><topic>Risk factors</topic><topic>Single nucleotide polymorphisms</topic><topic>Studies</topic><topic>Vitamin B</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nasr, Aml S</creatorcontrib><creatorcontrib>Sami, Rania M</creatorcontrib><creatorcontrib>Ibrahim, Noha Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medicine (ProQuest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and therapeutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nasr, Aml S</au><au>Sami, Rania M</au><au>Ibrahim, Noha Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylenetetrahydrofolate reductase gene polymorphisms (677C > T and 1298A > C) in Egyptian patients with non-Hodgkin lymphoma</atitle><jtitle>Journal of cancer research and therapeutics</jtitle><addtitle>J Cancer Res Ther</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>8</volume><issue>3</issue><spage>355</spage><epage>360</epage><pages>355-360</pages><issn>0973-1482</issn><eissn>1998-4138</eissn><abstract>Folate metabolism plays an essential role in Deoxyribonucleic acid (DNA) synthesis and methylation processes. Deviations in the flux of the folate may affect the susceptibility to various cancers including lymphoma.
The aim of this study was to investigate the genetic polymorphisms in 5, 10-methylenetetrahydrofolate reductase (MTHFR 677C/T and 1298A/C) and to evaluate its associations with the risk of Non Hodgkin lymphoma.
The study included 50 patients with diffuse large B cell lymphoma (DLBCL) as well as 50 age matched apparently healthy volunteers (as control). All the subjects included in the study were genotyped for the detection of the MTHFR gene polymorphisms (677C > T and 1298A > C) by using restriction fragment length polymorphism (PCR-RFLP).
There were highly statistically significant differences between the 2 groups with respect to results of PCR-RFLP for MTHFR 677C→T polymorphism for CC genotype (P value = 0.001), statistically significant differences for CT (P value = 0.048) and TT (P value = 0.038) genotypes; however, no statistically significant differences regarding CC/CT or TT/CT alleles (P value = 0.052). Also, there were highly statistically significant differences between the patient and control groups with regards to the results of MTHFR1298 A/C polymorphism for the AA, AC genotypes as well as the AA/AC and CC/AC alleles (P value < 0.0001), and statistically significant difference regarding CC genotype (P value 0.0192).
In conclusion, this study demonstrated a significant association between the MTHFR polymorphisms and the risk of DLBCL. Thus the study could support that folate intake together with the genetic basis may help in modifying the risk to lymphoma.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>23174714</pmid><doi>10.4103/0973-1482.103512</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Adult Aged Cardiovascular disease Colorectal cancer Deoxyribonucleic acid Dihydrofolate reductase Disease susceptibility DNA DNA methylation Egypt Enzymes Female Folic Acid - metabolism Gene Frequency Genes Genetic aspects Genetic Association Studies Genetic Predisposition to Disease Genotype Haplotypes Humans Informed consent Lymphoma Lymphoma, Large B-Cell, Diffuse - genetics Lymphoma, Non-Hodgkin - genetics Male Methylenetetrahydrofolate Reductase (NADPH2) - genetics Middle Aged Non-Hodgkin's lymphomas Nuclear medicine Physiological aspects Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Risk Risk factors Single nucleotide polymorphisms Studies Vitamin B Young Adult |
| title | Methylenetetrahydrofolate reductase gene polymorphisms (677C > T and 1298A > C) in Egyptian patients with non-Hodgkin lymphoma |
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