The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model
BACKGROUND:Bioabsorbable unfilled synthetic nerve conduits have been used in the reconstruction of small segmental nerve defects with variable results, especially in motor nerves. We hypothesized that providing a synthetic mimic of the Schwann cell basal lamina in the form of a collagen-glycosaminog...
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| Veröffentlicht in: | Journal of bone and joint surgery. American volume 2012-11, Vol.94 (22), p.2084-2091 |
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| creator | Lee, Joo-Yup Giusti, Guilherme Friedrich, Patricia F Archibald, Simon J Kemnitzer, John E Patel, Jignesh Desai, Namrata Bishop, Allen T Shin, Alexander Y |
| description | BACKGROUND:Bioabsorbable unfilled synthetic nerve conduits have been used in the reconstruction of small segmental nerve defects with variable results, especially in motor nerves. We hypothesized that providing a synthetic mimic of the Schwann cell basal lamina in the form of a collagen-glycosaminoglycan (GAG) matrix would improve the bridging of the nerve gap and functional motor recovery.
METHODS:A unilateral 10-mm sciatic nerve defect was created in eighty-eight male Lewis rats. Animals were randomly divided into four experimental groupsrepair with reversed autograft, reconstruction with collagen nerve conduit (1.5-mm NeuraGen, Integra LifeSciences), reconstruction with collagen nerve conduit filled with collagen matrix, and reconstruction with collagen nerve conduit filled with collagen-GAG (chondroitin-6-sulfate) matrix. Nerve regeneration was evaluated at twelve weeks on the basis of the compound muscle action potential, maximum isometric tetanic force, and wet muscle weight of the tibialis anterior muscle, the ankle contracture angle, and nerve histomorphometry.
RESULTS:The use of autograft resulted in significantly better motor recovery compared with the other experimental methods. Conduit filled with collagen-GAG matrix demonstrated superior results compared with empty conduit or conduit filled with collagen matrix with respect to all experimental parameters. Axon counts in the conduit filled with collagen-GAG matrix were not significantly different from those in the reversed autograft at twelve weeks after repair.
CONCLUSIONS:The addition of the synthetic collagen basal-lamina matrix with chondroitin-6-sulfate into the lumen of an entubulation repair significantly improved bridging of the nerve gap and functional motor recovery in a rat model.
CLINICAL RELEVANCE:Use of a nerve conduit filled with collagen-GAG matrix to bridge a motor or mixed nerve defect may result in superior functional motor recovery compared with commercially available empty collagen conduit. However, nerve autograft remains the gold standard for reconstruction of a segmental motor nerve defect. |
| doi_str_mv | 10.2106/JBJS.K.00658 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1197482291</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1197482291</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4099-ed89edc7ba23177df282ea05a3cc7a22214beda96c5c69e5232ab7cf945a2f0a3</originalsourceid><addsrcrecordid>eNpF0ctu1DAUBmALgehQ2LFG3iB10Qy-JE68hFFb6AVQKevojHPSGDzxYDsMfQTeGk9nWiRLvujzsc5vQl5zNhecqXfnH86_zS_mjKmqeUJmvJJVwWWjnpIZY4IXWlbVAXkR4w_GWFmy-jk5EJLXQgo1I39vBqQnfY8mUd_ThXcObnGknzH8xrwdu8mmSE-tc9jRjU3DoynO3J3xEVZ29Ld5CSO9ghTsH-pH-hWDXQ8YwNErn3zYF7zGfDGfJpuNHSnQa0hZdOhekmc9uIiv9vMh-X56crP4WFx-Ofu0eH9ZmJJpXWDXaOxMvYRtE3XXi0YgsAqkMTUIIXi5xA60MpVRGqvcJixr0-uyAtEzkIfkaFd3HfyvCWNqVzYazD2N6KfYcq7rshFC80yPd9QEH2PAvl0Hu4Jw13LWbsNvt-G3F-19-Jm_2VeelivsHvFD2hm83QOIBlwfYDQ2_ndKKS3LMrty5zbeJQzxp5s2GNoBwaUhP5b_UQlZCMYFz4MV2yMt_wFpnZ10</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1197482291</pqid></control><display><type>article</type><title>The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><source>Journals@Ovid Complete</source><creator>Lee, Joo-Yup ; Giusti, Guilherme ; Friedrich, Patricia F ; Archibald, Simon J ; Kemnitzer, John E ; Patel, Jignesh ; Desai, Namrata ; Bishop, Allen T ; Shin, Alexander Y</creator><creatorcontrib>Lee, Joo-Yup ; Giusti, Guilherme ; Friedrich, Patricia F ; Archibald, Simon J ; Kemnitzer, John E ; Patel, Jignesh ; Desai, Namrata ; Bishop, Allen T ; Shin, Alexander Y</creatorcontrib><description>BACKGROUND:Bioabsorbable unfilled synthetic nerve conduits have been used in the reconstruction of small segmental nerve defects with variable results, especially in motor nerves. We hypothesized that providing a synthetic mimic of the Schwann cell basal lamina in the form of a collagen-glycosaminoglycan (GAG) matrix would improve the bridging of the nerve gap and functional motor recovery.
METHODS:A unilateral 10-mm sciatic nerve defect was created in eighty-eight male Lewis rats. Animals were randomly divided into four experimental groupsrepair with reversed autograft, reconstruction with collagen nerve conduit (1.5-mm NeuraGen, Integra LifeSciences), reconstruction with collagen nerve conduit filled with collagen matrix, and reconstruction with collagen nerve conduit filled with collagen-GAG (chondroitin-6-sulfate) matrix. Nerve regeneration was evaluated at twelve weeks on the basis of the compound muscle action potential, maximum isometric tetanic force, and wet muscle weight of the tibialis anterior muscle, the ankle contracture angle, and nerve histomorphometry.
RESULTS:The use of autograft resulted in significantly better motor recovery compared with the other experimental methods. Conduit filled with collagen-GAG matrix demonstrated superior results compared with empty conduit or conduit filled with collagen matrix with respect to all experimental parameters. Axon counts in the conduit filled with collagen-GAG matrix were not significantly different from those in the reversed autograft at twelve weeks after repair.
CONCLUSIONS:The addition of the synthetic collagen basal-lamina matrix with chondroitin-6-sulfate into the lumen of an entubulation repair significantly improved bridging of the nerve gap and functional motor recovery in a rat model.
CLINICAL RELEVANCE:Use of a nerve conduit filled with collagen-GAG matrix to bridge a motor or mixed nerve defect may result in superior functional motor recovery compared with commercially available empty collagen conduit. However, nerve autograft remains the gold standard for reconstruction of a segmental motor nerve defect.</description><identifier>ISSN: 0021-9355</identifier><identifier>EISSN: 1535-1386</identifier><identifier>DOI: 10.2106/JBJS.K.00658</identifier><identifier>PMID: 23172326</identifier><identifier>CODEN: JBJSA3</identifier><language>eng</language><publisher>Boston, MA: Copyright by The Journal of Bone and Joint Surgery, Incorporated</publisher><subject>Absorbable Implants ; Animals ; Biological and medical sciences ; Chondroitin Sulfates - pharmacology ; Collagen - pharmacology ; Disease Models, Animal ; Diseases of the osteoarticular system ; Electromyography - methods ; Glycosaminoglycans - pharmacology ; Guided Tissue Regeneration ; Male ; Medical sciences ; Motor Skills - physiology ; Nerve Regeneration - physiology ; Orthopedic surgery ; Peripheral Nerves - drug effects ; Peripheral Nerves - pathology ; Peripheral Nerves - surgery ; Peripheral Nervous System Diseases - drug therapy ; Peripheral Nervous System Diseases - surgery ; Random Allocation ; Rats ; Rats, Inbred Lew ; Recovery of Function ; Reference Values ; Sciatic Nerve - drug effects ; Sciatic Nerve - pathology ; Sciatic Nerve - surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Transplantation, Autologous ; Treatment Outcome</subject><ispartof>Journal of bone and joint surgery. American volume, 2012-11, Vol.94 (22), p.2084-2091</ispartof><rights>Copyright 2012 by The Journal of Bone and Joint Surgery, Incorporated</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4099-ed89edc7ba23177df282ea05a3cc7a22214beda96c5c69e5232ab7cf945a2f0a3</citedby><cites>FETCH-LOGICAL-c4099-ed89edc7ba23177df282ea05a3cc7a22214beda96c5c69e5232ab7cf945a2f0a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26669344$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23172326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Joo-Yup</creatorcontrib><creatorcontrib>Giusti, Guilherme</creatorcontrib><creatorcontrib>Friedrich, Patricia F</creatorcontrib><creatorcontrib>Archibald, Simon J</creatorcontrib><creatorcontrib>Kemnitzer, John E</creatorcontrib><creatorcontrib>Patel, Jignesh</creatorcontrib><creatorcontrib>Desai, Namrata</creatorcontrib><creatorcontrib>Bishop, Allen T</creatorcontrib><creatorcontrib>Shin, Alexander Y</creatorcontrib><title>The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model</title><title>Journal of bone and joint surgery. American volume</title><addtitle>J Bone Joint Surg Am</addtitle><description>BACKGROUND:Bioabsorbable unfilled synthetic nerve conduits have been used in the reconstruction of small segmental nerve defects with variable results, especially in motor nerves. We hypothesized that providing a synthetic mimic of the Schwann cell basal lamina in the form of a collagen-glycosaminoglycan (GAG) matrix would improve the bridging of the nerve gap and functional motor recovery.
METHODS:A unilateral 10-mm sciatic nerve defect was created in eighty-eight male Lewis rats. Animals were randomly divided into four experimental groupsrepair with reversed autograft, reconstruction with collagen nerve conduit (1.5-mm NeuraGen, Integra LifeSciences), reconstruction with collagen nerve conduit filled with collagen matrix, and reconstruction with collagen nerve conduit filled with collagen-GAG (chondroitin-6-sulfate) matrix. Nerve regeneration was evaluated at twelve weeks on the basis of the compound muscle action potential, maximum isometric tetanic force, and wet muscle weight of the tibialis anterior muscle, the ankle contracture angle, and nerve histomorphometry.
RESULTS:The use of autograft resulted in significantly better motor recovery compared with the other experimental methods. Conduit filled with collagen-GAG matrix demonstrated superior results compared with empty conduit or conduit filled with collagen matrix with respect to all experimental parameters. Axon counts in the conduit filled with collagen-GAG matrix were not significantly different from those in the reversed autograft at twelve weeks after repair.
CONCLUSIONS:The addition of the synthetic collagen basal-lamina matrix with chondroitin-6-sulfate into the lumen of an entubulation repair significantly improved bridging of the nerve gap and functional motor recovery in a rat model.
CLINICAL RELEVANCE:Use of a nerve conduit filled with collagen-GAG matrix to bridge a motor or mixed nerve defect may result in superior functional motor recovery compared with commercially available empty collagen conduit. However, nerve autograft remains the gold standard for reconstruction of a segmental motor nerve defect.</description><subject>Absorbable Implants</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chondroitin Sulfates - pharmacology</subject><subject>Collagen - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Diseases of the osteoarticular system</subject><subject>Electromyography - methods</subject><subject>Glycosaminoglycans - pharmacology</subject><subject>Guided Tissue Regeneration</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Motor Skills - physiology</subject><subject>Nerve Regeneration - physiology</subject><subject>Orthopedic surgery</subject><subject>Peripheral Nerves - drug effects</subject><subject>Peripheral Nerves - pathology</subject><subject>Peripheral Nerves - surgery</subject><subject>Peripheral Nervous System Diseases - drug therapy</subject><subject>Peripheral Nervous System Diseases - surgery</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Recovery of Function</subject><subject>Reference Values</subject><subject>Sciatic Nerve - drug effects</subject><subject>Sciatic Nerve - pathology</subject><subject>Sciatic Nerve - surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Transplantation, Autologous</subject><subject>Treatment Outcome</subject><issn>0021-9355</issn><issn>1535-1386</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0ctu1DAUBmALgehQ2LFG3iB10Qy-JE68hFFb6AVQKevojHPSGDzxYDsMfQTeGk9nWiRLvujzsc5vQl5zNhecqXfnH86_zS_mjKmqeUJmvJJVwWWjnpIZY4IXWlbVAXkR4w_GWFmy-jk5EJLXQgo1I39vBqQnfY8mUd_ThXcObnGknzH8xrwdu8mmSE-tc9jRjU3DoynO3J3xEVZ29Ld5CSO9ghTsH-pH-hWDXQ8YwNErn3zYF7zGfDGfJpuNHSnQa0hZdOhekmc9uIiv9vMh-X56crP4WFx-Ofu0eH9ZmJJpXWDXaOxMvYRtE3XXi0YgsAqkMTUIIXi5xA60MpVRGqvcJixr0-uyAtEzkIfkaFd3HfyvCWNqVzYazD2N6KfYcq7rshFC80yPd9QEH2PAvl0Hu4Jw13LWbsNvt-G3F-19-Jm_2VeelivsHvFD2hm83QOIBlwfYDQ2_ndKKS3LMrty5zbeJQzxp5s2GNoBwaUhP5b_UQlZCMYFz4MV2yMt_wFpnZ10</recordid><startdate>20121121</startdate><enddate>20121121</enddate><creator>Lee, Joo-Yup</creator><creator>Giusti, Guilherme</creator><creator>Friedrich, Patricia F</creator><creator>Archibald, Simon J</creator><creator>Kemnitzer, John E</creator><creator>Patel, Jignesh</creator><creator>Desai, Namrata</creator><creator>Bishop, Allen T</creator><creator>Shin, Alexander Y</creator><general>Copyright by The Journal of Bone and Joint Surgery, Incorporated</general><general>Journal of Bone and Joint Surgery Incorporated</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121121</creationdate><title>The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model</title><author>Lee, Joo-Yup ; Giusti, Guilherme ; Friedrich, Patricia F ; Archibald, Simon J ; Kemnitzer, John E ; Patel, Jignesh ; Desai, Namrata ; Bishop, Allen T ; Shin, Alexander Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4099-ed89edc7ba23177df282ea05a3cc7a22214beda96c5c69e5232ab7cf945a2f0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Absorbable Implants</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chondroitin Sulfates - pharmacology</topic><topic>Collagen - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Diseases of the osteoarticular system</topic><topic>Electromyography - methods</topic><topic>Glycosaminoglycans - pharmacology</topic><topic>Guided Tissue Regeneration</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Motor Skills - physiology</topic><topic>Nerve Regeneration - physiology</topic><topic>Orthopedic surgery</topic><topic>Peripheral Nerves - drug effects</topic><topic>Peripheral Nerves - pathology</topic><topic>Peripheral Nerves - surgery</topic><topic>Peripheral Nervous System Diseases - drug therapy</topic><topic>Peripheral Nervous System Diseases - surgery</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Recovery of Function</topic><topic>Reference Values</topic><topic>Sciatic Nerve - drug effects</topic><topic>Sciatic Nerve - pathology</topic><topic>Sciatic Nerve - surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Transplantation, Autologous</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Joo-Yup</creatorcontrib><creatorcontrib>Giusti, Guilherme</creatorcontrib><creatorcontrib>Friedrich, Patricia F</creatorcontrib><creatorcontrib>Archibald, Simon J</creatorcontrib><creatorcontrib>Kemnitzer, John E</creatorcontrib><creatorcontrib>Patel, Jignesh</creatorcontrib><creatorcontrib>Desai, Namrata</creatorcontrib><creatorcontrib>Bishop, Allen T</creatorcontrib><creatorcontrib>Shin, Alexander Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bone and joint surgery. American volume</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Joo-Yup</au><au>Giusti, Guilherme</au><au>Friedrich, Patricia F</au><au>Archibald, Simon J</au><au>Kemnitzer, John E</au><au>Patel, Jignesh</au><au>Desai, Namrata</au><au>Bishop, Allen T</au><au>Shin, Alexander Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model</atitle><jtitle>Journal of bone and joint surgery. American volume</jtitle><addtitle>J Bone Joint Surg Am</addtitle><date>2012-11-21</date><risdate>2012</risdate><volume>94</volume><issue>22</issue><spage>2084</spage><epage>2091</epage><pages>2084-2091</pages><issn>0021-9355</issn><eissn>1535-1386</eissn><coden>JBJSA3</coden><abstract>BACKGROUND:Bioabsorbable unfilled synthetic nerve conduits have been used in the reconstruction of small segmental nerve defects with variable results, especially in motor nerves. We hypothesized that providing a synthetic mimic of the Schwann cell basal lamina in the form of a collagen-glycosaminoglycan (GAG) matrix would improve the bridging of the nerve gap and functional motor recovery.
METHODS:A unilateral 10-mm sciatic nerve defect was created in eighty-eight male Lewis rats. Animals were randomly divided into four experimental groupsrepair with reversed autograft, reconstruction with collagen nerve conduit (1.5-mm NeuraGen, Integra LifeSciences), reconstruction with collagen nerve conduit filled with collagen matrix, and reconstruction with collagen nerve conduit filled with collagen-GAG (chondroitin-6-sulfate) matrix. Nerve regeneration was evaluated at twelve weeks on the basis of the compound muscle action potential, maximum isometric tetanic force, and wet muscle weight of the tibialis anterior muscle, the ankle contracture angle, and nerve histomorphometry.
RESULTS:The use of autograft resulted in significantly better motor recovery compared with the other experimental methods. Conduit filled with collagen-GAG matrix demonstrated superior results compared with empty conduit or conduit filled with collagen matrix with respect to all experimental parameters. Axon counts in the conduit filled with collagen-GAG matrix were not significantly different from those in the reversed autograft at twelve weeks after repair.
CONCLUSIONS:The addition of the synthetic collagen basal-lamina matrix with chondroitin-6-sulfate into the lumen of an entubulation repair significantly improved bridging of the nerve gap and functional motor recovery in a rat model.
CLINICAL RELEVANCE:Use of a nerve conduit filled with collagen-GAG matrix to bridge a motor or mixed nerve defect may result in superior functional motor recovery compared with commercially available empty collagen conduit. However, nerve autograft remains the gold standard for reconstruction of a segmental motor nerve defect.</abstract><cop>Boston, MA</cop><pub>Copyright by The Journal of Bone and Joint Surgery, Incorporated</pub><pmid>23172326</pmid><doi>10.2106/JBJS.K.00658</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of bone and joint surgery. American volume, 2012-11, Vol.94 (22), p.2084-2091 |
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| subjects | Absorbable Implants Animals Biological and medical sciences Chondroitin Sulfates - pharmacology Collagen - pharmacology Disease Models, Animal Diseases of the osteoarticular system Electromyography - methods Glycosaminoglycans - pharmacology Guided Tissue Regeneration Male Medical sciences Motor Skills - physiology Nerve Regeneration - physiology Orthopedic surgery Peripheral Nerves - drug effects Peripheral Nerves - pathology Peripheral Nerves - surgery Peripheral Nervous System Diseases - drug therapy Peripheral Nervous System Diseases - surgery Random Allocation Rats Rats, Inbred Lew Recovery of Function Reference Values Sciatic Nerve - drug effects Sciatic Nerve - pathology Sciatic Nerve - surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Transplantation, Autologous Treatment Outcome |
| title | The Effect of Collagen Nerve Conduits Filled with Collagen-Glycosaminoglycan Matrix on Peripheral Motor Nerve Regeneration in a Rat Model |
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