Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR
Clostridial myositis is an acute, generally fatal toxemia that is considered to be rare in pet animals. The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium...
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| Veröffentlicht in: | Anaerobe 2012-10, Vol.18 (5), p.504-507 |
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| creator | Ribeiro, Márcio Garcia Silva, Rodrigo Otávio Silveira Pires, Prhiscylla Sadanã Martinho, Anna Paula Vitirito Lucas, Thays Mizuki Teixeira, Ana Izabel Passarela Paes, Antonio Carlos Barros, Claudenice Batista Lobato, Francisco Carlos Faria |
| description | Clostridial myositis is an acute, generally fatal toxemia that is considered to be rare in pet animals. The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium septicum, Clostridium perfringens type A, Clostridium chauvoei, and Clostridium novyi type A. A ten-month-old male Rottweiler dog, that had displayed lameness and swelling of the left limb for 12 h, was admitted to a veterinary hospital. The animal was weak, dyspneic and hyperthermic, and a clinical examination indicated the presence of gas and edema in the limb. Despite emergency treatment, the animal died in only a few minutes. Samples of muscular tissue from the necrotic area were aseptically collected and plated onto defibrinated sheep blood agar (5%) in anaerobic conditions. Colonies suggestive of Clostridium spp. were submitted to testing by multiplex-PCR. Impression smears of the tissues, visualized with Gram and also with panoptic stains, revealed long rod-shaped organisms, and specimens also tested positive using the fluorescent antibody technique (FAT). The FAT and mPCR tests enabled a diagnosis of C. septicum myonecrosis in the dog. |
| doi_str_mv | 10.1016/j.anaerobe.2012.09.003 |
| format | Article |
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The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium septicum, Clostridium perfringens type A, Clostridium chauvoei, and Clostridium novyi type A. A ten-month-old male Rottweiler dog, that had displayed lameness and swelling of the left limb for 12 h, was admitted to a veterinary hospital. The animal was weak, dyspneic and hyperthermic, and a clinical examination indicated the presence of gas and edema in the limb. Despite emergency treatment, the animal died in only a few minutes. Samples of muscular tissue from the necrotic area were aseptically collected and plated onto defibrinated sheep blood agar (5%) in anaerobic conditions. Colonies suggestive of Clostridium spp. were submitted to testing by multiplex-PCR. Impression smears of the tissues, visualized with Gram and also with panoptic stains, revealed long rod-shaped organisms, and specimens also tested positive using the fluorescent antibody technique (FAT). The FAT and mPCR tests enabled a diagnosis of C. septicum myonecrosis in the dog.</description><identifier>ISSN: 1075-9964</identifier><identifier>EISSN: 1095-8274</identifier><identifier>DOI: 10.1016/j.anaerobe.2012.09.003</identifier><identifier>PMID: 22975141</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Agar ; Anaerobic conditions ; Animals ; Antibodies ; Blood ; Canine ; clinical examination ; Clostridium ; Clostridium chauvoei ; Clostridium novyi ; Clostridium perfringens ; Clostridium perfringens A ; Clostridium septicum ; Clostridium septicum - genetics ; Clostridium septicum - isolation & purification ; Clostridium sordellii ; Colonies ; Dog Diseases - diagnosis ; Dog Diseases - microbiology ; Dogs ; Edema ; fluorescent antibody technique ; Gas gangrene ; Gas Gangrene - diagnosis ; Gas Gangrene - microbiology ; Gas Gangrene - veterinary ; Hospitals ; lameness ; Limbs ; Male ; males ; Molecular Diagnostic Techniques - methods ; Multiplex Polymerase Chain Reaction - methods ; Myonecrosis ; Myositis ; Myositis - diagnosis ; Myositis - microbiology ; Myositis - veterinary ; Necrosis - diagnosis ; Necrosis - microbiology ; Necrosis - veterinary ; pets ; Rottweiler ; sheep ; Stains ; tissues ; Toxemia</subject><ispartof>Anaerobe, 2012-10, Vol.18 (5), p.504-507</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-cbc95e2b9d277335d5061abe30efe18475beab7839362afad84d25db4ac1390c3</citedby><cites>FETCH-LOGICAL-c425t-cbc95e2b9d277335d5061abe30efe18475beab7839362afad84d25db4ac1390c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anaerobe.2012.09.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22975141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro, Márcio Garcia</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><creatorcontrib>Pires, Prhiscylla Sadanã</creatorcontrib><creatorcontrib>Martinho, Anna Paula Vitirito</creatorcontrib><creatorcontrib>Lucas, Thays Mizuki</creatorcontrib><creatorcontrib>Teixeira, Ana Izabel Passarela</creatorcontrib><creatorcontrib>Paes, Antonio Carlos</creatorcontrib><creatorcontrib>Barros, Claudenice Batista</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><title>Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR</title><title>Anaerobe</title><addtitle>Anaerobe</addtitle><description>Clostridial myositis is an acute, generally fatal toxemia that is considered to be rare in pet animals. The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium septicum, Clostridium perfringens type A, Clostridium chauvoei, and Clostridium novyi type A. A ten-month-old male Rottweiler dog, that had displayed lameness and swelling of the left limb for 12 h, was admitted to a veterinary hospital. The animal was weak, dyspneic and hyperthermic, and a clinical examination indicated the presence of gas and edema in the limb. Despite emergency treatment, the animal died in only a few minutes. Samples of muscular tissue from the necrotic area were aseptically collected and plated onto defibrinated sheep blood agar (5%) in anaerobic conditions. Colonies suggestive of Clostridium spp. were submitted to testing by multiplex-PCR. Impression smears of the tissues, visualized with Gram and also with panoptic stains, revealed long rod-shaped organisms, and specimens also tested positive using the fluorescent antibody technique (FAT). The FAT and mPCR tests enabled a diagnosis of C. septicum myonecrosis in the dog.</description><subject>Agar</subject><subject>Anaerobic conditions</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Blood</subject><subject>Canine</subject><subject>clinical examination</subject><subject>Clostridium</subject><subject>Clostridium chauvoei</subject><subject>Clostridium novyi</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens A</subject><subject>Clostridium septicum</subject><subject>Clostridium septicum - genetics</subject><subject>Clostridium septicum - isolation & purification</subject><subject>Clostridium sordellii</subject><subject>Colonies</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Edema</subject><subject>fluorescent antibody technique</subject><subject>Gas gangrene</subject><subject>Gas Gangrene - diagnosis</subject><subject>Gas Gangrene - microbiology</subject><subject>Gas Gangrene - veterinary</subject><subject>Hospitals</subject><subject>lameness</subject><subject>Limbs</subject><subject>Male</subject><subject>males</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Multiplex Polymerase Chain Reaction - methods</subject><subject>Myonecrosis</subject><subject>Myositis</subject><subject>Myositis - diagnosis</subject><subject>Myositis - microbiology</subject><subject>Myositis - veterinary</subject><subject>Necrosis - diagnosis</subject><subject>Necrosis - microbiology</subject><subject>Necrosis - veterinary</subject><subject>pets</subject><subject>Rottweiler</subject><subject>sheep</subject><subject>Stains</subject><subject>tissues</subject><subject>Toxemia</subject><issn>1075-9964</issn><issn>1095-8274</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtu1EAQRS0EIg_4heAlC-x09cvuHWgUEqQEEJB1qx_lUY9s99BtA_P3sTUJW1hVLc69VTpFcQGkBgLycleb0WCKFmtKgNZE1YSwZ8UpECWqljb8-bo3olJK8pPiLOcdIQBciJfFCaWqEcDhtPh8d4gjuhRzyKU9lJs-5ikFH-ahzLifgluWMJam9HH7rvTBbMeY0a-sKUf8XQ5zP4V9j3-qr5tvr4oXnekzvn6c58X9x6sfm5vq9sv1p82H28pxKqbKWacEUqs8bRrGhBdEgrHICHYILW-ERWOblikmqemMb7mnwltuHDBFHDsv3h579yn-nDFPegjZYd-bEeOcNUADrZJCwX-gAK1koOSCyiO6-sgJO71PYTDpoIHoVbve6SftetWuidKL9iV48XhjtgP6v7Enzwvw5gh0JmqzTSHr--9LgySECEFbvhDvjwQu2n4FTDq7gKNDHxK6SfsY_vXFA4inn1U</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Ribeiro, Márcio Garcia</creator><creator>Silva, Rodrigo Otávio Silveira</creator><creator>Pires, Prhiscylla Sadanã</creator><creator>Martinho, Anna Paula Vitirito</creator><creator>Lucas, Thays Mizuki</creator><creator>Teixeira, Ana Izabel Passarela</creator><creator>Paes, Antonio Carlos</creator><creator>Barros, Claudenice Batista</creator><creator>Lobato, Francisco Carlos Faria</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20121001</creationdate><title>Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR</title><author>Ribeiro, Márcio Garcia ; Silva, Rodrigo Otávio Silveira ; Pires, Prhiscylla Sadanã ; Martinho, Anna Paula Vitirito ; Lucas, Thays Mizuki ; Teixeira, Ana Izabel Passarela ; Paes, Antonio Carlos ; Barros, Claudenice Batista ; Lobato, Francisco Carlos Faria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-cbc95e2b9d277335d5061abe30efe18475beab7839362afad84d25db4ac1390c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agar</topic><topic>Anaerobic conditions</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Blood</topic><topic>Canine</topic><topic>clinical examination</topic><topic>Clostridium</topic><topic>Clostridium chauvoei</topic><topic>Clostridium novyi</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens A</topic><topic>Clostridium septicum</topic><topic>Clostridium septicum - genetics</topic><topic>Clostridium septicum - isolation & purification</topic><topic>Clostridium sordellii</topic><topic>Colonies</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Edema</topic><topic>fluorescent antibody technique</topic><topic>Gas gangrene</topic><topic>Gas Gangrene - diagnosis</topic><topic>Gas Gangrene - microbiology</topic><topic>Gas Gangrene - veterinary</topic><topic>Hospitals</topic><topic>lameness</topic><topic>Limbs</topic><topic>Male</topic><topic>males</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Multiplex Polymerase Chain Reaction - methods</topic><topic>Myonecrosis</topic><topic>Myositis</topic><topic>Myositis - diagnosis</topic><topic>Myositis - microbiology</topic><topic>Myositis - veterinary</topic><topic>Necrosis - diagnosis</topic><topic>Necrosis - microbiology</topic><topic>Necrosis - veterinary</topic><topic>pets</topic><topic>Rottweiler</topic><topic>sheep</topic><topic>Stains</topic><topic>tissues</topic><topic>Toxemia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro, Márcio Garcia</creatorcontrib><creatorcontrib>Silva, Rodrigo Otávio Silveira</creatorcontrib><creatorcontrib>Pires, Prhiscylla Sadanã</creatorcontrib><creatorcontrib>Martinho, Anna Paula Vitirito</creatorcontrib><creatorcontrib>Lucas, Thays Mizuki</creatorcontrib><creatorcontrib>Teixeira, Ana Izabel Passarela</creatorcontrib><creatorcontrib>Paes, Antonio Carlos</creatorcontrib><creatorcontrib>Barros, Claudenice Batista</creatorcontrib><creatorcontrib>Lobato, Francisco Carlos Faria</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Anaerobe</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro, Márcio Garcia</au><au>Silva, Rodrigo Otávio Silveira</au><au>Pires, Prhiscylla Sadanã</au><au>Martinho, Anna Paula Vitirito</au><au>Lucas, Thays Mizuki</au><au>Teixeira, Ana Izabel Passarela</au><au>Paes, Antonio Carlos</au><au>Barros, Claudenice Batista</au><au>Lobato, Francisco Carlos Faria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR</atitle><jtitle>Anaerobe</jtitle><addtitle>Anaerobe</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>18</volume><issue>5</issue><spage>504</spage><epage>507</epage><pages>504-507</pages><issn>1075-9964</issn><eissn>1095-8274</eissn><abstract>Clostridial myositis is an acute, generally fatal toxemia that is considered to be rare in pet animals. The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium septicum, Clostridium perfringens type A, Clostridium chauvoei, and Clostridium novyi type A. A ten-month-old male Rottweiler dog, that had displayed lameness and swelling of the left limb for 12 h, was admitted to a veterinary hospital. The animal was weak, dyspneic and hyperthermic, and a clinical examination indicated the presence of gas and edema in the limb. Despite emergency treatment, the animal died in only a few minutes. Samples of muscular tissue from the necrotic area were aseptically collected and plated onto defibrinated sheep blood agar (5%) in anaerobic conditions. Colonies suggestive of Clostridium spp. were submitted to testing by multiplex-PCR. Impression smears of the tissues, visualized with Gram and also with panoptic stains, revealed long rod-shaped organisms, and specimens also tested positive using the fluorescent antibody technique (FAT). The FAT and mPCR tests enabled a diagnosis of C. septicum myonecrosis in the dog.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22975141</pmid><doi>10.1016/j.anaerobe.2012.09.003</doi><tpages>4</tpages></addata></record> |
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| subjects | Agar Anaerobic conditions Animals Antibodies Blood Canine clinical examination Clostridium Clostridium chauvoei Clostridium novyi Clostridium perfringens Clostridium perfringens A Clostridium septicum Clostridium septicum - genetics Clostridium septicum - isolation & purification Clostridium sordellii Colonies Dog Diseases - diagnosis Dog Diseases - microbiology Dogs Edema fluorescent antibody technique Gas gangrene Gas Gangrene - diagnosis Gas Gangrene - microbiology Gas Gangrene - veterinary Hospitals lameness Limbs Male males Molecular Diagnostic Techniques - methods Multiplex Polymerase Chain Reaction - methods Myonecrosis Myositis Myositis - diagnosis Myositis - microbiology Myositis - veterinary Necrosis - diagnosis Necrosis - microbiology Necrosis - veterinary pets Rottweiler sheep Stains tissues Toxemia |
| title | Myonecrosis by Clostridium septicum in a dog, diagnosed by a new multiplex-PCR |
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