Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer
Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron micros...
Gespeichert in:
| Veröffentlicht in: | Poultry science 2012-12, Vol.91 (12), p.3202-3208 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3208 |
|---|---|
| container_issue | 12 |
| container_start_page | 3202 |
| container_title | Poultry science |
| container_volume | 91 |
| creator | Li, L Zhu, D K Zhou, Y Wang, M S Cheng, A C Jia, R Y Chen, S Liu, F Yang, Q M Chen, X Y |
| description | Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microfilaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks. |
| doi_str_mv | 10.3382/ps.2012-02552 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1171871819</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1171871819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c332t-25221e51cc85f22748d5da4735949e1d949020183218ac5c8d7b0849516f72503</originalsourceid><addsrcrecordid>eNo9UE1LxDAQzUFx19WjV8nRS9fMpNmmx2XxCxZE0XNIkxSj7bYmrbD_3uyHwjDDgzdv3jxCroDNOZd428c5MsCMoRB4QqaMccxEUcKEnMf4yRjCYlGckQlyEIJxmJKXpf1w0XcbqjeW-s2P3oOho3Y0X9S1Vdh2tPZV6KpGx4Ea1zSRVlv66l3rQkI6rerB9y4OvnbhgpzWuonu8jhn5P3-7m31mK2fH55Wy3VmOMchQ4EIToAxUtSIRS6tsDovuCjz0oFNnaVnJEeQ2ggjbVExmZcCFnWByfyM3Bx0-9B9j-m4an3cudMb141RARQgU0GZqNmBakIXY3C16oNvddgqYGqXnOqj2iWn9skl_vVReqxaZ__Zf7HxX5ZZaek</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1171871819</pqid></control><display><type>article</type><title>Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Li, L ; Zhu, D K ; Zhou, Y ; Wang, M S ; Cheng, A C ; Jia, R Y ; Chen, S ; Liu, F ; Yang, Q M ; Chen, X Y</creator><creatorcontrib>Li, L ; Zhu, D K ; Zhou, Y ; Wang, M S ; Cheng, A C ; Jia, R Y ; Chen, S ; Liu, F ; Yang, Q M ; Chen, X Y</creatorcontrib><description>Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microfilaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks.</description><identifier>ISSN: 0032-5791</identifier><identifier>DOI: 10.3382/ps.2012-02552</identifier><identifier>PMID: 23155031</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Bacterial Adhesion - physiology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Ducks - embryology ; Fibroblasts - microbiology ; Gene Expression Regulation, Bacterial - physiology ; Riemerella - physiology</subject><ispartof>Poultry science, 2012-12, Vol.91 (12), p.3202-3208</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-25221e51cc85f22748d5da4735949e1d949020183218ac5c8d7b0849516f72503</citedby><cites>FETCH-LOGICAL-c332t-25221e51cc85f22748d5da4735949e1d949020183218ac5c8d7b0849516f72503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23155031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, L</creatorcontrib><creatorcontrib>Zhu, D K</creatorcontrib><creatorcontrib>Zhou, Y</creatorcontrib><creatorcontrib>Wang, M S</creatorcontrib><creatorcontrib>Cheng, A C</creatorcontrib><creatorcontrib>Jia, R Y</creatorcontrib><creatorcontrib>Chen, S</creatorcontrib><creatorcontrib>Liu, F</creatorcontrib><creatorcontrib>Yang, Q M</creatorcontrib><creatorcontrib>Chen, X Y</creatorcontrib><title>Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer</title><title>Poultry science</title><addtitle>Poult Sci</addtitle><description>Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microfilaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks.</description><subject>Animals</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Ducks - embryology</subject><subject>Fibroblasts - microbiology</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>Riemerella - physiology</subject><issn>0032-5791</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UE1LxDAQzUFx19WjV8nRS9fMpNmmx2XxCxZE0XNIkxSj7bYmrbD_3uyHwjDDgzdv3jxCroDNOZd428c5MsCMoRB4QqaMccxEUcKEnMf4yRjCYlGckQlyEIJxmJKXpf1w0XcbqjeW-s2P3oOho3Y0X9S1Vdh2tPZV6KpGx4Ea1zSRVlv66l3rQkI6rerB9y4OvnbhgpzWuonu8jhn5P3-7m31mK2fH55Wy3VmOMchQ4EIToAxUtSIRS6tsDovuCjz0oFNnaVnJEeQ2ggjbVExmZcCFnWByfyM3Bx0-9B9j-m4an3cudMb141RARQgU0GZqNmBakIXY3C16oNvddgqYGqXnOqj2iWn9skl_vVReqxaZ__Zf7HxX5ZZaek</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Li, L</creator><creator>Zhu, D K</creator><creator>Zhou, Y</creator><creator>Wang, M S</creator><creator>Cheng, A C</creator><creator>Jia, R Y</creator><creator>Chen, S</creator><creator>Liu, F</creator><creator>Yang, Q M</creator><creator>Chen, X Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121201</creationdate><title>Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer</title><author>Li, L ; Zhu, D K ; Zhou, Y ; Wang, M S ; Cheng, A C ; Jia, R Y ; Chen, S ; Liu, F ; Yang, Q M ; Chen, X Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-25221e51cc85f22748d5da4735949e1d949020183218ac5c8d7b0849516f72503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Ducks - embryology</topic><topic>Fibroblasts - microbiology</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>Riemerella - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, L</creatorcontrib><creatorcontrib>Zhu, D K</creatorcontrib><creatorcontrib>Zhou, Y</creatorcontrib><creatorcontrib>Wang, M S</creatorcontrib><creatorcontrib>Cheng, A C</creatorcontrib><creatorcontrib>Jia, R Y</creatorcontrib><creatorcontrib>Chen, S</creatorcontrib><creatorcontrib>Liu, F</creatorcontrib><creatorcontrib>Yang, Q M</creatorcontrib><creatorcontrib>Chen, X Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Poultry science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, L</au><au>Zhu, D K</au><au>Zhou, Y</au><au>Wang, M S</au><au>Cheng, A C</au><au>Jia, R Y</au><au>Chen, S</au><au>Liu, F</au><au>Yang, Q M</au><au>Chen, X Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer</atitle><jtitle>Poultry science</jtitle><addtitle>Poult Sci</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>91</volume><issue>12</issue><spage>3202</spage><epage>3208</epage><pages>3202-3208</pages><issn>0032-5791</issn><abstract>Here, we investigated adhesion and invasion of Riemerella anatipestifer (RA) to primary duck embryo fibroblast (DEF) cells. The ability of RA to adhere to, and more importantly, to invade DEF cells was demonstrated by using a gentamicin invasion assay and was confirmed by transmission electron microscopy (TEM). Adhesion of RA could be found by TEM after 1 h of inoculation. Both apoptosis and necrocytosis of DEF were indicated by TEM after 10 h of incubation, which suggested a complex mechanism of DEF cell death induced by RA. Our results showed that internalized RA had the ability to leave the DEF cells. Inhibition studies indicated that RA proteins play a role in adhesion. Moreover, invasion of RA to DEF cells was shown to require rearrangement of actin microfilaments and microtubular cytoskeletal elements. Because the adhesion and invasion ability of RA to DEF cells could be demonstrated in vitro, similar processes might occur in vivo, where DEF cells play a crucial role in the diffusion of RA in ducks.</abstract><cop>England</cop><pmid>23155031</pmid><doi>10.3382/ps.2012-02552</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-5791 |
| ispartof | Poultry science, 2012-12, Vol.91 (12), p.3202-3208 |
| issn | 0032-5791 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1171871819 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Adhesion - physiology Bacterial Proteins - genetics Bacterial Proteins - metabolism Ducks - embryology Fibroblasts - microbiology Gene Expression Regulation, Bacterial - physiology Riemerella - physiology |
| title | Adhesion and invasion to duck embryo fibroblast cells by Riemerella anatipestifer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T02%3A50%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adhesion%20and%20invasion%20to%20duck%20embryo%20fibroblast%20cells%20by%20Riemerella%20anatipestifer&rft.jtitle=Poultry%20science&rft.au=Li,%20L&rft.date=2012-12-01&rft.volume=91&rft.issue=12&rft.spage=3202&rft.epage=3208&rft.pages=3202-3208&rft.issn=0032-5791&rft_id=info:doi/10.3382/ps.2012-02552&rft_dat=%3Cproquest_cross%3E1171871819%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1171871819&rft_id=info:pmid/23155031&rfr_iscdi=true |