Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment
OBJECTIVE: Patients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor beta 1 (TGF beta 1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation...
Gespeichert in:
| Veröffentlicht in: | European journal of endocrinology 2011-09, Vol.165 (3), p.393-400 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 400 |
|---|---|
| container_issue | 3 |
| container_start_page | 393 |
| container_title | European journal of endocrinology |
| container_volume | 165 |
| creator | Ueland, Thor Lekva, Tove Otterdal, Kari Dahl, Tuva B Olarescu, Nicoleta Cristina Joergensen, Anders P Fougner, Kristian J Brixen, Kim Aukrust, Paal Bollerslev, J |
| description | OBJECTIVE: Patients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor beta 1 (TGF beta 1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation by GH/IGF1 in vivo and in vitro. DESIGN AND METHODS: The effects of GH substitution (9-12 months, placebo controlled) on circulating and cortical bone matrix contents of TGF beta 1 were investigated in patients with aoGHD. The effects of GH/IGF1 on TGF beta 1 secretion in osteoblasts (hFOB), adipocytes, and THP-1 macrophages as well as the effects on release from platelets were investigated in vitro. RESULTS: In vivo GH substitution increased TGF beta 1 protein levels in cortical bone and serum. In vitro, GH/IGF1 stimulation induced a significant increase in TGF beta 1 secretion in hFOB. In contrast, no major effect of GH/IGF1 on TGF beta 1 was found in adipocytes and THP-1 macrophages. Finally, a minor modifying effect on SFLLRN-stimulated platelet release of TGF beta 1 was observed in the presence of IGF1. CONCLUSION: GH substitution increases TGF beta 1 in vivo and in vitro, and this effect could contribute to improved bone metabolism during such therapy, potentially reflecting direct effect of GH/IGF1 on bone cells. |
| doi_str_mv | 10.1530/EJE-11-0442 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1171868617</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1171868617</sourcerecordid><originalsourceid>FETCH-LOGICAL-p118t-807157bc86d987dc0f5df7a0902b0a3611ecc91dd08c87c722dd0b845bd1ebda3</originalsourceid><addsrcrecordid>eNotjctOwzAURL0AiVJY8QN3ySbgm5fdJapKW1SJDawrP25KUGIH26HwA3w3qWA1ozmjGcZukN9hVfD71dMqQ8x4WeZnbMYlL7OyLosLdhnjO-c4eT5jP1tnAqlIFiKFsQflLGjvCHqVQvsFHX1SF8E3kIJysfGhb90BDsEf0xs0yiQfQFNSgNA6GFRqyaUIx3bC6w1YalozReb7hAPFwbtIkPwJpuk69VP_ip03qot0_a9z9vq4ellust3zert82GUDokyZ5AIroY2s7UIKa3hT2UYovuC55qqoEcmYBVrLpZHCiDyfrJZlpS2StqqYs9u_3SH4j5Fi2vdtNNR1ypEf4x5RoKxljaL4BcQ9ZGw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1171868617</pqid></control><display><type>article</type><title>Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment</title><source>Oxford Academic Journals (OUP)</source><creator>Ueland, Thor ; Lekva, Tove ; Otterdal, Kari ; Dahl, Tuva B ; Olarescu, Nicoleta Cristina ; Joergensen, Anders P ; Fougner, Kristian J ; Brixen, Kim ; Aukrust, Paal ; Bollerslev, J</creator><creatorcontrib>Ueland, Thor ; Lekva, Tove ; Otterdal, Kari ; Dahl, Tuva B ; Olarescu, Nicoleta Cristina ; Joergensen, Anders P ; Fougner, Kristian J ; Brixen, Kim ; Aukrust, Paal ; Bollerslev, J</creatorcontrib><description>OBJECTIVE: Patients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor beta 1 (TGF beta 1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation by GH/IGF1 in vivo and in vitro. DESIGN AND METHODS: The effects of GH substitution (9-12 months, placebo controlled) on circulating and cortical bone matrix contents of TGF beta 1 were investigated in patients with aoGHD. The effects of GH/IGF1 on TGF beta 1 secretion in osteoblasts (hFOB), adipocytes, and THP-1 macrophages as well as the effects on release from platelets were investigated in vitro. RESULTS: In vivo GH substitution increased TGF beta 1 protein levels in cortical bone and serum. In vitro, GH/IGF1 stimulation induced a significant increase in TGF beta 1 secretion in hFOB. In contrast, no major effect of GH/IGF1 on TGF beta 1 was found in adipocytes and THP-1 macrophages. Finally, a minor modifying effect on SFLLRN-stimulated platelet release of TGF beta 1 was observed in the presence of IGF1. CONCLUSION: GH substitution increases TGF beta 1 in vivo and in vitro, and this effect could contribute to improved bone metabolism during such therapy, potentially reflecting direct effect of GH/IGF1 on bone cells.</description><identifier>ISSN: 0804-4643</identifier><identifier>DOI: 10.1530/EJE-11-0442</identifier><language>eng</language><subject>Adipocytes ; Bone (cortical) ; Bone growth ; Bone matrix ; Bone turnover ; Growth hormone ; Insulin-like growth factor I ; Insulin-like growth factors ; Macrophages ; Osteoblasts ; Osteoporosis ; Platelets ; Secretion ; Transforming growth factor- beta 1</subject><ispartof>European journal of endocrinology, 2011-09, Vol.165 (3), p.393-400</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Ueland, Thor</creatorcontrib><creatorcontrib>Lekva, Tove</creatorcontrib><creatorcontrib>Otterdal, Kari</creatorcontrib><creatorcontrib>Dahl, Tuva B</creatorcontrib><creatorcontrib>Olarescu, Nicoleta Cristina</creatorcontrib><creatorcontrib>Joergensen, Anders P</creatorcontrib><creatorcontrib>Fougner, Kristian J</creatorcontrib><creatorcontrib>Brixen, Kim</creatorcontrib><creatorcontrib>Aukrust, Paal</creatorcontrib><creatorcontrib>Bollerslev, J</creatorcontrib><title>Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment</title><title>European journal of endocrinology</title><description>OBJECTIVE: Patients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor beta 1 (TGF beta 1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation by GH/IGF1 in vivo and in vitro. DESIGN AND METHODS: The effects of GH substitution (9-12 months, placebo controlled) on circulating and cortical bone matrix contents of TGF beta 1 were investigated in patients with aoGHD. The effects of GH/IGF1 on TGF beta 1 secretion in osteoblasts (hFOB), adipocytes, and THP-1 macrophages as well as the effects on release from platelets were investigated in vitro. RESULTS: In vivo GH substitution increased TGF beta 1 protein levels in cortical bone and serum. In vitro, GH/IGF1 stimulation induced a significant increase in TGF beta 1 secretion in hFOB. In contrast, no major effect of GH/IGF1 on TGF beta 1 was found in adipocytes and THP-1 macrophages. Finally, a minor modifying effect on SFLLRN-stimulated platelet release of TGF beta 1 was observed in the presence of IGF1. CONCLUSION: GH substitution increases TGF beta 1 in vivo and in vitro, and this effect could contribute to improved bone metabolism during such therapy, potentially reflecting direct effect of GH/IGF1 on bone cells.</description><subject>Adipocytes</subject><subject>Bone (cortical)</subject><subject>Bone growth</subject><subject>Bone matrix</subject><subject>Bone turnover</subject><subject>Growth hormone</subject><subject>Insulin-like growth factor I</subject><subject>Insulin-like growth factors</subject><subject>Macrophages</subject><subject>Osteoblasts</subject><subject>Osteoporosis</subject><subject>Platelets</subject><subject>Secretion</subject><subject>Transforming growth factor- beta 1</subject><issn>0804-4643</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNotjctOwzAURL0AiVJY8QN3ySbgm5fdJapKW1SJDawrP25KUGIH26HwA3w3qWA1ozmjGcZukN9hVfD71dMqQ8x4WeZnbMYlL7OyLosLdhnjO-c4eT5jP1tnAqlIFiKFsQflLGjvCHqVQvsFHX1SF8E3kIJysfGhb90BDsEf0xs0yiQfQFNSgNA6GFRqyaUIx3bC6w1YalozReb7hAPFwbtIkPwJpuk69VP_ip03qot0_a9z9vq4ellust3zert82GUDokyZ5AIroY2s7UIKa3hT2UYovuC55qqoEcmYBVrLpZHCiDyfrJZlpS2StqqYs9u_3SH4j5Fi2vdtNNR1ypEf4x5RoKxljaL4BcQ9ZGw</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Ueland, Thor</creator><creator>Lekva, Tove</creator><creator>Otterdal, Kari</creator><creator>Dahl, Tuva B</creator><creator>Olarescu, Nicoleta Cristina</creator><creator>Joergensen, Anders P</creator><creator>Fougner, Kristian J</creator><creator>Brixen, Kim</creator><creator>Aukrust, Paal</creator><creator>Bollerslev, J</creator><scope>7QP</scope></search><sort><creationdate>20110901</creationdate><title>Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment</title><author>Ueland, Thor ; Lekva, Tove ; Otterdal, Kari ; Dahl, Tuva B ; Olarescu, Nicoleta Cristina ; Joergensen, Anders P ; Fougner, Kristian J ; Brixen, Kim ; Aukrust, Paal ; Bollerslev, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p118t-807157bc86d987dc0f5df7a0902b0a3611ecc91dd08c87c722dd0b845bd1ebda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipocytes</topic><topic>Bone (cortical)</topic><topic>Bone growth</topic><topic>Bone matrix</topic><topic>Bone turnover</topic><topic>Growth hormone</topic><topic>Insulin-like growth factor I</topic><topic>Insulin-like growth factors</topic><topic>Macrophages</topic><topic>Osteoblasts</topic><topic>Osteoporosis</topic><topic>Platelets</topic><topic>Secretion</topic><topic>Transforming growth factor- beta 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ueland, Thor</creatorcontrib><creatorcontrib>Lekva, Tove</creatorcontrib><creatorcontrib>Otterdal, Kari</creatorcontrib><creatorcontrib>Dahl, Tuva B</creatorcontrib><creatorcontrib>Olarescu, Nicoleta Cristina</creatorcontrib><creatorcontrib>Joergensen, Anders P</creatorcontrib><creatorcontrib>Fougner, Kristian J</creatorcontrib><creatorcontrib>Brixen, Kim</creatorcontrib><creatorcontrib>Aukrust, Paal</creatorcontrib><creatorcontrib>Bollerslev, J</creatorcontrib><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>European journal of endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ueland, Thor</au><au>Lekva, Tove</au><au>Otterdal, Kari</au><au>Dahl, Tuva B</au><au>Olarescu, Nicoleta Cristina</au><au>Joergensen, Anders P</au><au>Fougner, Kristian J</au><au>Brixen, Kim</au><au>Aukrust, Paal</au><au>Bollerslev, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment</atitle><jtitle>European journal of endocrinology</jtitle><date>2011-09-01</date><risdate>2011</risdate><volume>165</volume><issue>3</issue><spage>393</spage><epage>400</epage><pages>393-400</pages><issn>0804-4643</issn><abstract>OBJECTIVE: Patients with adult onset GH deficiency (aoGHD) have secondary osteoporosis, which is reversed by long-term GH substitution. Transforming growth factor beta 1 (TGF beta 1 or TGFB1) is abundant in bone tissue and could mediate some effects of GH/IGFs on bone. We investigated its regulation by GH/IGF1 in vivo and in vitro. DESIGN AND METHODS: The effects of GH substitution (9-12 months, placebo controlled) on circulating and cortical bone matrix contents of TGF beta 1 were investigated in patients with aoGHD. The effects of GH/IGF1 on TGF beta 1 secretion in osteoblasts (hFOB), adipocytes, and THP-1 macrophages as well as the effects on release from platelets were investigated in vitro. RESULTS: In vivo GH substitution increased TGF beta 1 protein levels in cortical bone and serum. In vitro, GH/IGF1 stimulation induced a significant increase in TGF beta 1 secretion in hFOB. In contrast, no major effect of GH/IGF1 on TGF beta 1 was found in adipocytes and THP-1 macrophages. Finally, a minor modifying effect on SFLLRN-stimulated platelet release of TGF beta 1 was observed in the presence of IGF1. CONCLUSION: GH substitution increases TGF beta 1 in vivo and in vitro, and this effect could contribute to improved bone metabolism during such therapy, potentially reflecting direct effect of GH/IGF1 on bone cells.</abstract><doi>10.1530/EJE-11-0442</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0804-4643 |
| ispartof | European journal of endocrinology, 2011-09, Vol.165 (3), p.393-400 |
| issn | 0804-4643 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1171868617 |
| source | Oxford Academic Journals (OUP) |
| subjects | Adipocytes Bone (cortical) Bone growth Bone matrix Bone turnover Growth hormone Insulin-like growth factor I Insulin-like growth factors Macrophages Osteoblasts Osteoporosis Platelets Secretion Transforming growth factor- beta 1 |
| title | Increased serum and bone matrix levels of transforming growth factor beta 1 in patients with GH deficiency in response to GH treatment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T16%3A46%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increased%20serum%20and%20bone%20matrix%20levels%20of%20transforming%20growth%20factor%20beta%201%20in%20patients%20with%20GH%20deficiency%20in%20response%20to%20GH%20treatment&rft.jtitle=European%20journal%20of%20endocrinology&rft.au=Ueland,%20Thor&rft.date=2011-09-01&rft.volume=165&rft.issue=3&rft.spage=393&rft.epage=400&rft.pages=393-400&rft.issn=0804-4643&rft_id=info:doi/10.1530/EJE-11-0442&rft_dat=%3Cproquest%3E1171868617%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1171868617&rft_id=info:pmid/&rfr_iscdi=true |