Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women

PPARδ is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPARδ content with blood lipids and lipoprot...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:American journal of physiology: endocrinology and metabolism 2012-11, Vol.303 (10), p.E1212-E1221
Hauptverfasser: Greene, Nicholas P, Fluckey, James D, Lambert, Brad S, Greene, Elizabeth S, Riechman, Steven E, Crouse, Stephen F
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page E1221
container_issue 10
container_start_page E1212
container_title American journal of physiology: endocrinology and metabolism
container_volume 303
creator Greene, Nicholas P
Fluckey, James D
Lambert, Brad S
Greene, Elizabeth S
Riechman, Steven E
Crouse, Stephen F
description PPARδ is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPARδ content with blood lipids and lipoproteins before and following exercise, overweight and obese men (n = 9) and women (n = 7) were recruited; age, BMI, body fat percentage, and Vo(2max) were (means ± SE) 45 ± 2.5 yr, 31.9 ± 1.4 kg/m(-2), 41.1 ± 1.5%, and 26.0 ± 1.3 mLO(2)·kg(-1)·min(-1), respectively. Subjects performed 12 wk of endurance exercise training (3 sessions/wk, progressing to 500 kcal/session). To assess the acute exercise response, subjects performed a single exercise session on a treadmill (70% Vo(2max), 400 kcal energy expenditure) before and after training. Muscle and blood samples were obtained prior to any exercise and 24 h after each acute exercise session. Muscle was analyzed for protein content of PPARδ, PPARα, PGC-1α, AMPKα, and the oxidative and lipoprotein markers FAT/CD36, CPT I, COX-IV, LPL, F(1) ATPase, ABCAI, and LDL receptor. Blood was assessed for lipids and lipoproteins. Repeated-measures ANOVA revealed no influence of sex on measured outcomes. PPARδ, PGC-1α, FAT/CD36, and LPL content were enhanced following acute exercise, whereas PPARα, AMPKα, CPT I, and COX-IV content were enhanced only after exercise training. PPARδ content negatively correlated with total and LDL cholesterol concentrations primarily in the untrained condition (r ≤ -0.4946, P < 0.05), whereas AMPKα was positively correlated with HDL cholesterol concentrations regardless of exercise (r ≥ 0.5543, P < 0.05). Our findings demonstrate exercise-induced expression of skeletal muscle PPARs and their target proteins, and this expression is associated with improved blood lipids and lipoproteins in obese adults.
doi_str_mv 10.1152/ajpendo.00309.2012
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1171859960</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1171859960</sourcerecordid><originalsourceid>FETCH-LOGICAL-c303t-c85191cf1aae0b39493ff2097e78a61c7dd3feb9abd0bb7616a0407cdfdff7253</originalsourceid><addsrcrecordid>eNp1kc1OGzEURq0KVELaF-ii8pIFE67tzEy8QhEqFBFEhNr1yD_XYDQZp_YMKW_DQ_Q5-kw1IXTHytf6vnt0pUPIFwYTxkp-oh7W2NkwARAgJxwY_0BGOeAFK8tyj4yASVGw2VQekMOUHgCgLqf8IzngXMr8qUbk-Rbvhlb1ISYaHNVtCJa2fu1toqrbjmEdQ4--S6d0uZzf_v2zDebXy6tj6js7GLRUP1H8jdH4hMdURaQmxIiZm7ON7-_fQ2YADY8YN-jv7vuToDEhXWG3LW5Cnj6RfafahJ9375j8PP_24-x7sbi5uDybLwojQPSFmZVMMuOYUghayKkUznGQNdYzVTFTWyscaqm0Ba3rilUKplAb66xzNS_FmBy9cvNpvwZMfbPyyWDbqg7DkBrGajYrpawgV_lr1cSQUkTXrKNfqfjUMGhezDQ7M83WTPNiJi993fEHvUL7f-VNhfgHNoiPZA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1171859960</pqid></control><display><type>article</type><title>Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women</title><source>MEDLINE</source><source>American Physiological Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Greene, Nicholas P ; Fluckey, James D ; Lambert, Brad S ; Greene, Elizabeth S ; Riechman, Steven E ; Crouse, Stephen F</creator><creatorcontrib>Greene, Nicholas P ; Fluckey, James D ; Lambert, Brad S ; Greene, Elizabeth S ; Riechman, Steven E ; Crouse, Stephen F</creatorcontrib><description>PPARδ is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPARδ content with blood lipids and lipoproteins before and following exercise, overweight and obese men (n = 9) and women (n = 7) were recruited; age, BMI, body fat percentage, and Vo(2max) were (means ± SE) 45 ± 2.5 yr, 31.9 ± 1.4 kg/m(-2), 41.1 ± 1.5%, and 26.0 ± 1.3 mLO(2)·kg(-1)·min(-1), respectively. Subjects performed 12 wk of endurance exercise training (3 sessions/wk, progressing to 500 kcal/session). To assess the acute exercise response, subjects performed a single exercise session on a treadmill (70% Vo(2max), 400 kcal energy expenditure) before and after training. Muscle and blood samples were obtained prior to any exercise and 24 h after each acute exercise session. Muscle was analyzed for protein content of PPARδ, PPARα, PGC-1α, AMPKα, and the oxidative and lipoprotein markers FAT/CD36, CPT I, COX-IV, LPL, F(1) ATPase, ABCAI, and LDL receptor. Blood was assessed for lipids and lipoproteins. Repeated-measures ANOVA revealed no influence of sex on measured outcomes. PPARδ, PGC-1α, FAT/CD36, and LPL content were enhanced following acute exercise, whereas PPARα, AMPKα, CPT I, and COX-IV content were enhanced only after exercise training. PPARδ content negatively correlated with total and LDL cholesterol concentrations primarily in the untrained condition (r ≤ -0.4946, P &lt; 0.05), whereas AMPKα was positively correlated with HDL cholesterol concentrations regardless of exercise (r ≥ 0.5543, P &lt; 0.05). Our findings demonstrate exercise-induced expression of skeletal muscle PPARs and their target proteins, and this expression is associated with improved blood lipids and lipoproteins in obese adults.</description><identifier>ISSN: 0193-1849</identifier><identifier>EISSN: 1522-1555</identifier><identifier>DOI: 10.1152/ajpendo.00309.2012</identifier><identifier>PMID: 22990076</identifier><language>eng</language><publisher>United States</publisher><subject>Adenylate Kinase - blood ; Adenylate Kinase - metabolism ; ATP Binding Cassette Transporter 1 ; ATP-Binding Cassette Transporters - blood ; Biopsy ; Blotting, Western ; CD36 Antigens - blood ; Cholesterol - blood ; Cohort Studies ; Energy Metabolism - physiology ; Exercise - physiology ; Female ; Heat-Shock Proteins - blood ; Humans ; Lipids - blood ; Lipoprotein Lipase - blood ; Lipoproteins - blood ; Male ; Middle Aged ; Muscle, Skeletal - enzymology ; Muscle, Skeletal - metabolism ; Obesity - blood ; Obesity - enzymology ; Obesity - metabolism ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ; PPAR alpha - blood ; PPAR delta - metabolism ; Proton-Translocating ATPases - blood ; Receptors, LDL - blood ; Statistics, Nonparametric ; Transcription Factors - blood</subject><ispartof>American journal of physiology: endocrinology and metabolism, 2012-11, Vol.303 (10), p.E1212-E1221</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-c85191cf1aae0b39493ff2097e78a61c7dd3feb9abd0bb7616a0407cdfdff7253</citedby><cites>FETCH-LOGICAL-c303t-c85191cf1aae0b39493ff2097e78a61c7dd3feb9abd0bb7616a0407cdfdff7253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22990076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Greene, Nicholas P</creatorcontrib><creatorcontrib>Fluckey, James D</creatorcontrib><creatorcontrib>Lambert, Brad S</creatorcontrib><creatorcontrib>Greene, Elizabeth S</creatorcontrib><creatorcontrib>Riechman, Steven E</creatorcontrib><creatorcontrib>Crouse, Stephen F</creatorcontrib><title>Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women</title><title>American journal of physiology: endocrinology and metabolism</title><addtitle>Am J Physiol Endocrinol Metab</addtitle><description>PPARδ is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPARδ content with blood lipids and lipoproteins before and following exercise, overweight and obese men (n = 9) and women (n = 7) were recruited; age, BMI, body fat percentage, and Vo(2max) were (means ± SE) 45 ± 2.5 yr, 31.9 ± 1.4 kg/m(-2), 41.1 ± 1.5%, and 26.0 ± 1.3 mLO(2)·kg(-1)·min(-1), respectively. Subjects performed 12 wk of endurance exercise training (3 sessions/wk, progressing to 500 kcal/session). To assess the acute exercise response, subjects performed a single exercise session on a treadmill (70% Vo(2max), 400 kcal energy expenditure) before and after training. Muscle and blood samples were obtained prior to any exercise and 24 h after each acute exercise session. Muscle was analyzed for protein content of PPARδ, PPARα, PGC-1α, AMPKα, and the oxidative and lipoprotein markers FAT/CD36, CPT I, COX-IV, LPL, F(1) ATPase, ABCAI, and LDL receptor. Blood was assessed for lipids and lipoproteins. Repeated-measures ANOVA revealed no influence of sex on measured outcomes. PPARδ, PGC-1α, FAT/CD36, and LPL content were enhanced following acute exercise, whereas PPARα, AMPKα, CPT I, and COX-IV content were enhanced only after exercise training. PPARδ content negatively correlated with total and LDL cholesterol concentrations primarily in the untrained condition (r ≤ -0.4946, P &lt; 0.05), whereas AMPKα was positively correlated with HDL cholesterol concentrations regardless of exercise (r ≥ 0.5543, P &lt; 0.05). Our findings demonstrate exercise-induced expression of skeletal muscle PPARs and their target proteins, and this expression is associated with improved blood lipids and lipoproteins in obese adults.</description><subject>Adenylate Kinase - blood</subject><subject>Adenylate Kinase - metabolism</subject><subject>ATP Binding Cassette Transporter 1</subject><subject>ATP-Binding Cassette Transporters - blood</subject><subject>Biopsy</subject><subject>Blotting, Western</subject><subject>CD36 Antigens - blood</subject><subject>Cholesterol - blood</subject><subject>Cohort Studies</subject><subject>Energy Metabolism - physiology</subject><subject>Exercise - physiology</subject><subject>Female</subject><subject>Heat-Shock Proteins - blood</subject><subject>Humans</subject><subject>Lipids - blood</subject><subject>Lipoprotein Lipase - blood</subject><subject>Lipoproteins - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Muscle, Skeletal - enzymology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Obesity - blood</subject><subject>Obesity - enzymology</subject><subject>Obesity - metabolism</subject><subject>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha</subject><subject>PPAR alpha - blood</subject><subject>PPAR delta - metabolism</subject><subject>Proton-Translocating ATPases - blood</subject><subject>Receptors, LDL - blood</subject><subject>Statistics, Nonparametric</subject><subject>Transcription Factors - blood</subject><issn>0193-1849</issn><issn>1522-1555</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1OGzEURq0KVELaF-ii8pIFE67tzEy8QhEqFBFEhNr1yD_XYDQZp_YMKW_DQ_Q5-kw1IXTHytf6vnt0pUPIFwYTxkp-oh7W2NkwARAgJxwY_0BGOeAFK8tyj4yASVGw2VQekMOUHgCgLqf8IzngXMr8qUbk-Rbvhlb1ISYaHNVtCJa2fu1toqrbjmEdQ4--S6d0uZzf_v2zDebXy6tj6js7GLRUP1H8jdH4hMdURaQmxIiZm7ON7-_fQ2YADY8YN-jv7vuToDEhXWG3LW5Cnj6RfafahJ9375j8PP_24-x7sbi5uDybLwojQPSFmZVMMuOYUghayKkUznGQNdYzVTFTWyscaqm0Ba3rilUKplAb66xzNS_FmBy9cvNpvwZMfbPyyWDbqg7DkBrGajYrpawgV_lr1cSQUkTXrKNfqfjUMGhezDQ7M83WTPNiJi993fEHvUL7f-VNhfgHNoiPZA</recordid><startdate>20121115</startdate><enddate>20121115</enddate><creator>Greene, Nicholas P</creator><creator>Fluckey, James D</creator><creator>Lambert, Brad S</creator><creator>Greene, Elizabeth S</creator><creator>Riechman, Steven E</creator><creator>Crouse, Stephen F</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121115</creationdate><title>Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women</title><author>Greene, Nicholas P ; Fluckey, James D ; Lambert, Brad S ; Greene, Elizabeth S ; Riechman, Steven E ; Crouse, Stephen F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-c85191cf1aae0b39493ff2097e78a61c7dd3feb9abd0bb7616a0407cdfdff7253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenylate Kinase - blood</topic><topic>Adenylate Kinase - metabolism</topic><topic>ATP Binding Cassette Transporter 1</topic><topic>ATP-Binding Cassette Transporters - blood</topic><topic>Biopsy</topic><topic>Blotting, Western</topic><topic>CD36 Antigens - blood</topic><topic>Cholesterol - blood</topic><topic>Cohort Studies</topic><topic>Energy Metabolism - physiology</topic><topic>Exercise - physiology</topic><topic>Female</topic><topic>Heat-Shock Proteins - blood</topic><topic>Humans</topic><topic>Lipids - blood</topic><topic>Lipoprotein Lipase - blood</topic><topic>Lipoproteins - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Muscle, Skeletal - enzymology</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Obesity - blood</topic><topic>Obesity - enzymology</topic><topic>Obesity - metabolism</topic><topic>Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha</topic><topic>PPAR alpha - blood</topic><topic>PPAR delta - metabolism</topic><topic>Proton-Translocating ATPases - blood</topic><topic>Receptors, LDL - blood</topic><topic>Statistics, Nonparametric</topic><topic>Transcription Factors - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Greene, Nicholas P</creatorcontrib><creatorcontrib>Fluckey, James D</creatorcontrib><creatorcontrib>Lambert, Brad S</creatorcontrib><creatorcontrib>Greene, Elizabeth S</creatorcontrib><creatorcontrib>Riechman, Steven E</creatorcontrib><creatorcontrib>Crouse, Stephen F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology: endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Greene, Nicholas P</au><au>Fluckey, James D</au><au>Lambert, Brad S</au><au>Greene, Elizabeth S</au><au>Riechman, Steven E</au><au>Crouse, Stephen F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women</atitle><jtitle>American journal of physiology: endocrinology and metabolism</jtitle><addtitle>Am J Physiol Endocrinol Metab</addtitle><date>2012-11-15</date><risdate>2012</risdate><volume>303</volume><issue>10</issue><spage>E1212</spage><epage>E1221</epage><pages>E1212-E1221</pages><issn>0193-1849</issn><eissn>1522-1555</eissn><abstract>PPARδ is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPARδ content with blood lipids and lipoproteins before and following exercise, overweight and obese men (n = 9) and women (n = 7) were recruited; age, BMI, body fat percentage, and Vo(2max) were (means ± SE) 45 ± 2.5 yr, 31.9 ± 1.4 kg/m(-2), 41.1 ± 1.5%, and 26.0 ± 1.3 mLO(2)·kg(-1)·min(-1), respectively. Subjects performed 12 wk of endurance exercise training (3 sessions/wk, progressing to 500 kcal/session). To assess the acute exercise response, subjects performed a single exercise session on a treadmill (70% Vo(2max), 400 kcal energy expenditure) before and after training. Muscle and blood samples were obtained prior to any exercise and 24 h after each acute exercise session. Muscle was analyzed for protein content of PPARδ, PPARα, PGC-1α, AMPKα, and the oxidative and lipoprotein markers FAT/CD36, CPT I, COX-IV, LPL, F(1) ATPase, ABCAI, and LDL receptor. Blood was assessed for lipids and lipoproteins. Repeated-measures ANOVA revealed no influence of sex on measured outcomes. PPARδ, PGC-1α, FAT/CD36, and LPL content were enhanced following acute exercise, whereas PPARα, AMPKα, CPT I, and COX-IV content were enhanced only after exercise training. PPARδ content negatively correlated with total and LDL cholesterol concentrations primarily in the untrained condition (r ≤ -0.4946, P &lt; 0.05), whereas AMPKα was positively correlated with HDL cholesterol concentrations regardless of exercise (r ≥ 0.5543, P &lt; 0.05). Our findings demonstrate exercise-induced expression of skeletal muscle PPARs and their target proteins, and this expression is associated with improved blood lipids and lipoproteins in obese adults.</abstract><cop>United States</cop><pmid>22990076</pmid><doi>10.1152/ajpendo.00309.2012</doi></addata></record>
fulltext fulltext
identifier ISSN: 0193-1849
ispartof American journal of physiology: endocrinology and metabolism, 2012-11, Vol.303 (10), p.E1212-E1221
issn 0193-1849
1522-1555
language eng
recordid cdi_proquest_miscellaneous_1171859960
source MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Adenylate Kinase - blood
Adenylate Kinase - metabolism
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters - blood
Biopsy
Blotting, Western
CD36 Antigens - blood
Cholesterol - blood
Cohort Studies
Energy Metabolism - physiology
Exercise - physiology
Female
Heat-Shock Proteins - blood
Humans
Lipids - blood
Lipoprotein Lipase - blood
Lipoproteins - blood
Male
Middle Aged
Muscle, Skeletal - enzymology
Muscle, Skeletal - metabolism
Obesity - blood
Obesity - enzymology
Obesity - metabolism
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
PPAR alpha - blood
PPAR delta - metabolism
Proton-Translocating ATPases - blood
Receptors, LDL - blood
Statistics, Nonparametric
Transcription Factors - blood
title Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T02%3A46%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Regulators%20of%20blood%20lipids%20and%20lipoproteins?%20PPAR%CE%B4%20and%20AMPK,%20induced%20by%20exercise,%20are%20correlated%20with%20lipids%20and%20lipoproteins%20in%20overweight/obese%20men%20and%20women&rft.jtitle=American%20journal%20of%20physiology:%20endocrinology%20and%20metabolism&rft.au=Greene,%20Nicholas%20P&rft.date=2012-11-15&rft.volume=303&rft.issue=10&rft.spage=E1212&rft.epage=E1221&rft.pages=E1212-E1221&rft.issn=0193-1849&rft.eissn=1522-1555&rft_id=info:doi/10.1152/ajpendo.00309.2012&rft_dat=%3Cproquest_cross%3E1171859960%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1171859960&rft_id=info:pmid/22990076&rfr_iscdi=true