Detection of vimentin serine phosphorylation by multicolor Quenchbodies

Protein phosphorylation is a key event in intracellular signal transduction, and fluorescent biosensor for the specific phosphorylation event in a target protein is considered highly useful as a tool of cellular biology and drug screening. Vimentin, the most abundant intermediate filament protein, i...

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Veröffentlicht in:	Biosensors & bioelectronics 2013-02, Vol.40 (1), p.17-23
Hauptverfasser:	Jeong, Hee-Jin, Ohmuro-Matsuyama, Yuki, Ohashi, Hiroyuki, Ohsawa, Fuyuki, Tatsu, Yoshiro, Inagaki, Masaki, Ueda, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Equipment Design Equipment Failure Analysis Fluorescence quenching Fundamental and applied biological sciences. Psychology Immunoassay - instrumentation Methods. Procedures. Technologies Microscopy, Fluorescence, Multiphoton - instrumentation Phosphorylation Protein phosphorylation Quenchbody Reproducibility of Results Sensitivity and Specificity Serine - analysis Serine - chemistry Single chain antibody Spectrometry, Fluorescence - instrumentation Various methods and equipments Vimentin Vimentin - analysis Vimentin - chemistry
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