Evidence of Selective Packaging and Different α-Granule Subtypes in Canine Platelets
Platelets are circulating megakaryocytic cytoplasmic fragments that are required to maintain vascular integrity and prevent haemorrhage. During activation, platelets release hundreds of bioactive proteins, mainly by secretion of α-granule content. These proteins include von Willebrand factor (vWF) a...
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| Veröffentlicht in: | Journal of comparative pathology 2012-11, Vol.147 (4), p.499-502 |
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| container_title | Journal of comparative pathology |
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| creator | Curotto, S. Lunsford, K. Smith, W. Thomason, J. Bulla, C. |
| description | Platelets are circulating megakaryocytic cytoplasmic fragments that are required to maintain vascular integrity and prevent haemorrhage. During activation, platelets release hundreds of bioactive proteins, mainly by secretion of α-granule content. These proteins include von Willebrand factor (vWF) and fibrinogen, major adhesive proteins involved in haemostasis. Human and mouse platelet α-granules are packaged selectively to contain different molecules and platelets can differentially release these proteins on specific receptor activation. Confocal laser scanning microscopy was used to analyse vWF and fibrinogen localization within canine platelet α-granules. In resting canine platelets, the majority of platelet vWF and fibrinogen is located in separate α-granule populations. These findings provide evidence that canine platelets contain different α-granule subtypes, suggesting that selective protein packaging occurs during canine platelet ontology. |
| doi_str_mv | 10.1016/j.jcpa.2012.02.007 |
| format | Article |
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During activation, platelets release hundreds of bioactive proteins, mainly by secretion of α-granule content. These proteins include von Willebrand factor (vWF) and fibrinogen, major adhesive proteins involved in haemostasis. Human and mouse platelet α-granules are packaged selectively to contain different molecules and platelets can differentially release these proteins on specific receptor activation. Confocal laser scanning microscopy was used to analyse vWF and fibrinogen localization within canine platelet α-granules. In resting canine platelets, the majority of platelet vWF and fibrinogen is located in separate α-granule populations. These findings provide evidence that canine platelets contain different α-granule subtypes, suggesting that selective protein packaging occurs during canine platelet ontology.</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1016/j.jcpa.2012.02.007</identifier><identifier>PMID: 22516083</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; bioactive properties ; blood coagulation factors ; Blood Platelets - cytology ; Blood Platelets - secretion ; Clinical Chemistry Tests - veterinary ; confocal laser scanning microscopy ; confocal microscopy ; Cytoplasmic Granules - classification ; Cytoplasmic Granules - metabolism ; Cytoplasmic Granules - ultrastructure ; dog ; Dogs ; Female ; fibrinogen ; Fibrinogen - metabolism ; Hematologic Tests - veterinary ; hemorrhage ; hemostasis ; Humans ; Mice ; Microscopy, Confocal - methods ; packaging ; platelet ; secretion ; Species Specificity ; von Willebrand Factor - metabolism ; α-granule</subject><ispartof>Journal of comparative pathology, 2012-11, Vol.147 (4), p.499-502</ispartof><rights>2012</rights><rights>Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-eee86fafc1d7fba21cc67d0091b22f799d629707badee034b79d95cfeafce2ca3</citedby><cites>FETCH-LOGICAL-c380t-eee86fafc1d7fba21cc67d0091b22f799d629707badee034b79d95cfeafce2ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021997512000461$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22516083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Curotto, S.</creatorcontrib><creatorcontrib>Lunsford, K.</creatorcontrib><creatorcontrib>Smith, W.</creatorcontrib><creatorcontrib>Thomason, J.</creatorcontrib><creatorcontrib>Bulla, C.</creatorcontrib><title>Evidence of Selective Packaging and Different α-Granule Subtypes in Canine Platelets</title><title>Journal of comparative pathology</title><addtitle>J Comp Pathol</addtitle><description>Platelets are circulating megakaryocytic cytoplasmic fragments that are required to maintain vascular integrity and prevent haemorrhage. During activation, platelets release hundreds of bioactive proteins, mainly by secretion of α-granule content. These proteins include von Willebrand factor (vWF) and fibrinogen, major adhesive proteins involved in haemostasis. Human and mouse platelet α-granules are packaged selectively to contain different molecules and platelets can differentially release these proteins on specific receptor activation. Confocal laser scanning microscopy was used to analyse vWF and fibrinogen localization within canine platelet α-granules. In resting canine platelets, the majority of platelet vWF and fibrinogen is located in separate α-granule populations. These findings provide evidence that canine platelets contain different α-granule subtypes, suggesting that selective protein packaging occurs during canine platelet ontology.</description><subject>Animals</subject><subject>bioactive properties</subject><subject>blood coagulation factors</subject><subject>Blood Platelets - cytology</subject><subject>Blood Platelets - secretion</subject><subject>Clinical Chemistry Tests - veterinary</subject><subject>confocal laser scanning microscopy</subject><subject>confocal microscopy</subject><subject>Cytoplasmic Granules - classification</subject><subject>Cytoplasmic Granules - metabolism</subject><subject>Cytoplasmic Granules - ultrastructure</subject><subject>dog</subject><subject>Dogs</subject><subject>Female</subject><subject>fibrinogen</subject><subject>Fibrinogen - metabolism</subject><subject>Hematologic Tests - veterinary</subject><subject>hemorrhage</subject><subject>hemostasis</subject><subject>Humans</subject><subject>Mice</subject><subject>Microscopy, Confocal - methods</subject><subject>packaging</subject><subject>platelet</subject><subject>secretion</subject><subject>Species Specificity</subject><subject>von Willebrand Factor - metabolism</subject><subject>α-granule</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFqGzEURUVoSdy0P9BFo2U24z5pqtEIuilOmhYCLbheC430ZOSONY40Y8hn9UfyTdHgtMvChbc59_I4hLxnsGTAmo-75c4ezJID40soAXlGFkzUvKoZV6_IAoCzSikpLsibnHcA0LaCn5MLzgVroK0XZHN7DA6jRTp4usYe7RiOSH8a-9tsQ9xSEx29Cd5jwjjSpz_VXTJx6pGup258PGCmIdKViSGWVm_GMjHmt-S1N33Gdy_3kmy-3v5afavuf9x9X325r2zdwlghYtt44y1z0neGM2sb6QAU6zj3UinXcCVBdsYhQv2pk8opYT2WCnJr6ktyfdo9pOFhwjzqfcgW-95EHKasGRNMAqtlU1B-Qm0ack7o9SGFvUmPmoGedeqdnnXqWaeGEpCl9OFlf-r26P5V_vorwNUJ8GbQZptC1pt1WRCza9E2ohCfTwQWD8eASWcbZuEupCJbuyH874NnDwSRCA</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Curotto, S.</creator><creator>Lunsford, K.</creator><creator>Smith, W.</creator><creator>Thomason, J.</creator><creator>Bulla, C.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121101</creationdate><title>Evidence of Selective Packaging and Different α-Granule Subtypes in Canine Platelets</title><author>Curotto, S. ; Lunsford, K. ; Smith, W. ; Thomason, J. ; Bulla, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-eee86fafc1d7fba21cc67d0091b22f799d629707badee034b79d95cfeafce2ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>bioactive properties</topic><topic>blood coagulation factors</topic><topic>Blood Platelets - cytology</topic><topic>Blood Platelets - secretion</topic><topic>Clinical Chemistry Tests - veterinary</topic><topic>confocal laser scanning microscopy</topic><topic>confocal microscopy</topic><topic>Cytoplasmic Granules - classification</topic><topic>Cytoplasmic Granules - metabolism</topic><topic>Cytoplasmic Granules - ultrastructure</topic><topic>dog</topic><topic>Dogs</topic><topic>Female</topic><topic>fibrinogen</topic><topic>Fibrinogen - metabolism</topic><topic>Hematologic Tests - veterinary</topic><topic>hemorrhage</topic><topic>hemostasis</topic><topic>Humans</topic><topic>Mice</topic><topic>Microscopy, Confocal - methods</topic><topic>packaging</topic><topic>platelet</topic><topic>secretion</topic><topic>Species Specificity</topic><topic>von Willebrand Factor - metabolism</topic><topic>α-granule</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Curotto, S.</creatorcontrib><creatorcontrib>Lunsford, K.</creatorcontrib><creatorcontrib>Smith, W.</creatorcontrib><creatorcontrib>Thomason, J.</creatorcontrib><creatorcontrib>Bulla, C.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Curotto, S.</au><au>Lunsford, K.</au><au>Smith, W.</au><au>Thomason, J.</au><au>Bulla, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of Selective Packaging and Different α-Granule Subtypes in Canine Platelets</atitle><jtitle>Journal of comparative pathology</jtitle><addtitle>J Comp Pathol</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>147</volume><issue>4</issue><spage>499</spage><epage>502</epage><pages>499-502</pages><issn>0021-9975</issn><eissn>1532-3129</eissn><abstract>Platelets are circulating megakaryocytic cytoplasmic fragments that are required to maintain vascular integrity and prevent haemorrhage. During activation, platelets release hundreds of bioactive proteins, mainly by secretion of α-granule content. These proteins include von Willebrand factor (vWF) and fibrinogen, major adhesive proteins involved in haemostasis. Human and mouse platelet α-granules are packaged selectively to contain different molecules and platelets can differentially release these proteins on specific receptor activation. Confocal laser scanning microscopy was used to analyse vWF and fibrinogen localization within canine platelet α-granules. In resting canine platelets, the majority of platelet vWF and fibrinogen is located in separate α-granule populations. These findings provide evidence that canine platelets contain different α-granule subtypes, suggesting that selective protein packaging occurs during canine platelet ontology.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22516083</pmid><doi>10.1016/j.jcpa.2012.02.007</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of comparative pathology, 2012-11, Vol.147 (4), p.499-502 |
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| subjects | Animals bioactive properties blood coagulation factors Blood Platelets - cytology Blood Platelets - secretion Clinical Chemistry Tests - veterinary confocal laser scanning microscopy confocal microscopy Cytoplasmic Granules - classification Cytoplasmic Granules - metabolism Cytoplasmic Granules - ultrastructure dog Dogs Female fibrinogen Fibrinogen - metabolism Hematologic Tests - veterinary hemorrhage hemostasis Humans Mice Microscopy, Confocal - methods packaging platelet secretion Species Specificity von Willebrand Factor - metabolism α-granule |
| title | Evidence of Selective Packaging and Different α-Granule Subtypes in Canine Platelets |
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