Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans
In modern times, there has been a major increase in the use of plants or herbal constituents for the prevention of age-related disorders. 4-Hydroxy-E-globularinin (4-HEG) is an iridoid and a major component of Premna integrifolia. This investigation represents a breakthrough in geriatrics by showing...
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| Veröffentlicht in: | Free radical biology & medicine 2012-11, Vol.53 (10), p.1848-1856 |
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| creator | Shukla, Virendra Yadav, Deepti Phulara, Suresh C. Gupta, M.M. Saikia, Shilpi K. Pandey, Rakesh |
| description | In modern times, there has been a major increase in the use of plants or herbal constituents for the prevention of age-related disorders. 4-Hydroxy-E-globularinin (4-HEG) is an iridoid and a major component of Premna integrifolia. This investigation represents a breakthrough in geriatrics by showing the longevity-promoting activity of 4-HEG in the animal model Caenorhabditis elegans. 4-HEG (20μM) enhanced the mean life span of worms by over 18.8% under normal culture conditions and also enhanced their survival under oxidative stress. The longevity-promoting activity was associated with reduced reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies verified the role of ROS detoxification pathways and simultaneous nuclear translocation of DAF-16 in the 4-HEG-mediated effects. Quantitative real-time PCR estimations and observations of transcriptional reporters indicated that 4-HEG was able to upregulate stress-inducible genes, viz., hsp-16.2 and sod-3. Thus, 4-HEG may serve as a lead compound of plant origin for the development of important nutraceuticals superseding the aging process.
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► 4-Hydroxy-E-globularinin (4-HEG) is a natural molecule isolated from the medicinal plant Premna integrifolia. ► We examined the life span of wild-type N2 Caenorhabditis elegans and daf-16, sir-2.1, and mev-1 mutants. ► 4-HEG reduced reactive oxygen species levels and fat accumulation in the worms. ► 4-HEG extended the life span of worms via DAF-16 activation. |
| doi_str_mv | 10.1016/j.freeradbiomed.2012.08.594 |
| format | Article |
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[Display omitted]
► 4-Hydroxy-E-globularinin (4-HEG) is a natural molecule isolated from the medicinal plant Premna integrifolia. ► We examined the life span of wild-type N2 Caenorhabditis elegans and daf-16, sir-2.1, and mev-1 mutants. ► 4-HEG reduced reactive oxygen species levels and fat accumulation in the worms. ► 4-HEG extended the life span of worms via DAF-16 activation.</description><identifier>ISSN: 0891-5849</identifier><identifier>EISSN: 1873-4596</identifier><identifier>DOI: 10.1016/j.freeradbiomed.2012.08.594</identifier><identifier>PMID: 23000058</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>4-Hydroxy-E-globularinin ; Aging ; Aging - drug effects ; animal models ; Animals ; Caenorhabditis elegans ; Caenorhabditis elegans - drug effects ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - metabolism ; Caenorhabditis elegans - physiology ; Caenorhabditis elegans Proteins - biosynthesis ; Caenorhabditis elegans Proteins - genetics ; Caenorhabditis elegans Proteins - metabolism ; Chemotaxis - drug effects ; Forkhead Transcription Factors ; Free radicals ; Free Radicals - metabolism ; functional foods ; Gene Expression Regulation - drug effects ; genes ; Genes, Helminth ; Heat-Shock Proteins - biosynthesis ; Heat-Shock Proteins - genetics ; Iridoid ; Iridoids - chemistry ; Iridoids - pharmacology ; Life Expectancy ; Life span ; longevity ; Longevity - drug effects ; Models, Animal ; mutants ; oxidative stress ; Oxidative Stress - drug effects ; Plant Bark - metabolism ; Premna ; quantitative polymerase chain reaction ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Signal Transduction ; superoxide dismutase ; transcription (genetics) ; Transcription Factors - metabolism ; Transcriptional Activation</subject><ispartof>Free radical biology & medicine, 2012-11, Vol.53 (10), p.1848-1856</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-846250584cda40b08f9173dce9b867e29db7957633db221a2f9a17bbf44c7cd3</citedby><cites>FETCH-LOGICAL-c473t-846250584cda40b08f9173dce9b867e29db7957633db221a2f9a17bbf44c7cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.freeradbiomed.2012.08.594$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23000058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shukla, Virendra</creatorcontrib><creatorcontrib>Yadav, Deepti</creatorcontrib><creatorcontrib>Phulara, Suresh C.</creatorcontrib><creatorcontrib>Gupta, M.M.</creatorcontrib><creatorcontrib>Saikia, Shilpi K.</creatorcontrib><creatorcontrib>Pandey, Rakesh</creatorcontrib><title>Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans</title><title>Free radical biology & medicine</title><addtitle>Free Radic Biol Med</addtitle><description>In modern times, there has been a major increase in the use of plants or herbal constituents for the prevention of age-related disorders. 4-Hydroxy-E-globularinin (4-HEG) is an iridoid and a major component of Premna integrifolia. This investigation represents a breakthrough in geriatrics by showing the longevity-promoting activity of 4-HEG in the animal model Caenorhabditis elegans. 4-HEG (20μM) enhanced the mean life span of worms by over 18.8% under normal culture conditions and also enhanced their survival under oxidative stress. The longevity-promoting activity was associated with reduced reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies verified the role of ROS detoxification pathways and simultaneous nuclear translocation of DAF-16 in the 4-HEG-mediated effects. Quantitative real-time PCR estimations and observations of transcriptional reporters indicated that 4-HEG was able to upregulate stress-inducible genes, viz., hsp-16.2 and sod-3. Thus, 4-HEG may serve as a lead compound of plant origin for the development of important nutraceuticals superseding the aging process.
[Display omitted]
► 4-Hydroxy-E-globularinin (4-HEG) is a natural molecule isolated from the medicinal plant Premna integrifolia. ► We examined the life span of wild-type N2 Caenorhabditis elegans and daf-16, sir-2.1, and mev-1 mutants. ► 4-HEG reduced reactive oxygen species levels and fat accumulation in the worms. ► 4-HEG extended the life span of worms via DAF-16 activation.</description><subject>4-Hydroxy-E-globularinin</subject><subject>Aging</subject><subject>Aging - drug effects</subject><subject>animal models</subject><subject>Animals</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - drug effects</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - metabolism</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Caenorhabditis elegans Proteins - biosynthesis</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Caenorhabditis elegans Proteins - metabolism</subject><subject>Chemotaxis - drug effects</subject><subject>Forkhead Transcription Factors</subject><subject>Free radicals</subject><subject>Free Radicals - metabolism</subject><subject>functional foods</subject><subject>Gene Expression Regulation - drug effects</subject><subject>genes</subject><subject>Genes, Helminth</subject><subject>Heat-Shock Proteins - biosynthesis</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Iridoid</subject><subject>Iridoids - chemistry</subject><subject>Iridoids - pharmacology</subject><subject>Life Expectancy</subject><subject>Life span</subject><subject>longevity</subject><subject>Longevity - drug effects</subject><subject>Models, Animal</subject><subject>mutants</subject><subject>oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Plant Bark - metabolism</subject><subject>Premna</subject><subject>quantitative polymerase chain reaction</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction</subject><subject>superoxide dismutase</subject><subject>transcription (genetics)</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptional Activation</subject><issn>0891-5849</issn><issn>1873-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1rGzEQhkVpaNy0f6Fd6KUXbaSVdiXRUzHuBxhKSXIW-hhtZNarVFqH-N9Xxsmht8KABvTMzMuD0CdKWkrocL1rQwbIxtuY9uDbjtCuJbLtFX-FVlQKhnmvhtdoRaSiuJdcXaK3pewIIbxn8g267FjtSS9X6Pc2zSM8xuWIH3LapyXOYwMhgFtKk0LD8f3R5_R0xBs8TskeJpPjHOem1trAnPK9sT4usTQwwWjm8g5dBDMVeP_8XqHbb5vb9Q-8_fX95_rrFjsu2IIlH7q-RuDOG04skUFRwbwDZeUgoFPeCtWLgTFvu46aLihDhbWBcyecZ1fo83ltjf3nAGXR-1gcTJOZIR2KprSnhPFBqYp-OaMup1IyBP2Q497ko6ZEn5Tqnf5HqT4p1UTqqrROf3g-dLCnv5fZF4cV-HgGgknajDkWfXdTN_SEUCYoI5XYnAmoPh4jZF1chNmBj7mK1j7F_4ryFxXrmSA</recordid><startdate>20121115</startdate><enddate>20121115</enddate><creator>Shukla, Virendra</creator><creator>Yadav, Deepti</creator><creator>Phulara, Suresh C.</creator><creator>Gupta, M.M.</creator><creator>Saikia, Shilpi K.</creator><creator>Pandey, Rakesh</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121115</creationdate><title>Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans</title><author>Shukla, Virendra ; Yadav, Deepti ; Phulara, Suresh C. ; Gupta, M.M. ; Saikia, Shilpi K. ; Pandey, Rakesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-846250584cda40b08f9173dce9b867e29db7957633db221a2f9a17bbf44c7cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>4-Hydroxy-E-globularinin</topic><topic>Aging</topic><topic>Aging - drug effects</topic><topic>animal models</topic><topic>Animals</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - drug effects</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - metabolism</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Caenorhabditis elegans Proteins - biosynthesis</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Caenorhabditis elegans Proteins - metabolism</topic><topic>Chemotaxis - drug effects</topic><topic>Forkhead Transcription Factors</topic><topic>Free radicals</topic><topic>Free Radicals - metabolism</topic><topic>functional foods</topic><topic>Gene Expression Regulation - drug effects</topic><topic>genes</topic><topic>Genes, Helminth</topic><topic>Heat-Shock Proteins - biosynthesis</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Iridoid</topic><topic>Iridoids - chemistry</topic><topic>Iridoids - pharmacology</topic><topic>Life Expectancy</topic><topic>Life span</topic><topic>longevity</topic><topic>Longevity - drug effects</topic><topic>Models, Animal</topic><topic>mutants</topic><topic>oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Plant Bark - metabolism</topic><topic>Premna</topic><topic>quantitative polymerase chain reaction</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction</topic><topic>superoxide dismutase</topic><topic>transcription (genetics)</topic><topic>Transcription Factors - metabolism</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shukla, Virendra</creatorcontrib><creatorcontrib>Yadav, Deepti</creatorcontrib><creatorcontrib>Phulara, Suresh C.</creatorcontrib><creatorcontrib>Gupta, M.M.</creatorcontrib><creatorcontrib>Saikia, Shilpi K.</creatorcontrib><creatorcontrib>Pandey, Rakesh</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shukla, Virendra</au><au>Yadav, Deepti</au><au>Phulara, Suresh C.</au><au>Gupta, M.M.</au><au>Saikia, Shilpi K.</au><au>Pandey, Rakesh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans</atitle><jtitle>Free radical biology & medicine</jtitle><addtitle>Free Radic Biol Med</addtitle><date>2012-11-15</date><risdate>2012</risdate><volume>53</volume><issue>10</issue><spage>1848</spage><epage>1856</epage><pages>1848-1856</pages><issn>0891-5849</issn><eissn>1873-4596</eissn><abstract>In modern times, there has been a major increase in the use of plants or herbal constituents for the prevention of age-related disorders. 4-Hydroxy-E-globularinin (4-HEG) is an iridoid and a major component of Premna integrifolia. This investigation represents a breakthrough in geriatrics by showing the longevity-promoting activity of 4-HEG in the animal model Caenorhabditis elegans. 4-HEG (20μM) enhanced the mean life span of worms by over 18.8% under normal culture conditions and also enhanced their survival under oxidative stress. The longevity-promoting activity was associated with reduced reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies verified the role of ROS detoxification pathways and simultaneous nuclear translocation of DAF-16 in the 4-HEG-mediated effects. Quantitative real-time PCR estimations and observations of transcriptional reporters indicated that 4-HEG was able to upregulate stress-inducible genes, viz., hsp-16.2 and sod-3. Thus, 4-HEG may serve as a lead compound of plant origin for the development of important nutraceuticals superseding the aging process.
[Display omitted]
► 4-Hydroxy-E-globularinin (4-HEG) is a natural molecule isolated from the medicinal plant Premna integrifolia. ► We examined the life span of wild-type N2 Caenorhabditis elegans and daf-16, sir-2.1, and mev-1 mutants. ► 4-HEG reduced reactive oxygen species levels and fat accumulation in the worms. ► 4-HEG extended the life span of worms via DAF-16 activation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23000058</pmid><doi>10.1016/j.freeradbiomed.2012.08.594</doi><tpages>9</tpages></addata></record> |
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| subjects | 4-Hydroxy-E-globularinin Aging Aging - drug effects animal models Animals Caenorhabditis elegans Caenorhabditis elegans - drug effects Caenorhabditis elegans - genetics Caenorhabditis elegans - metabolism Caenorhabditis elegans - physiology Caenorhabditis elegans Proteins - biosynthesis Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Chemotaxis - drug effects Forkhead Transcription Factors Free radicals Free Radicals - metabolism functional foods Gene Expression Regulation - drug effects genes Genes, Helminth Heat-Shock Proteins - biosynthesis Heat-Shock Proteins - genetics Iridoid Iridoids - chemistry Iridoids - pharmacology Life Expectancy Life span longevity Longevity - drug effects Models, Animal mutants oxidative stress Oxidative Stress - drug effects Plant Bark - metabolism Premna quantitative polymerase chain reaction Reactive oxygen species Reactive Oxygen Species - metabolism Signal Transduction superoxide dismutase transcription (genetics) Transcription Factors - metabolism Transcriptional Activation |
| title | Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans |
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