Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF‑7 Cells through Endoplasmic Reticulum Stress
Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of pro...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2012-11, Vol.60 (44), p.11065-11070 |
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| container_issue | 44 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Kamiya, Tetsuro Nishihara, Hiroko Hara, Hirokazu Adachi, Tetsuo |
| description | Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of BRP induces MCF-7 cell apoptosis through, at least in part, ER stress-related signaling. |
| doi_str_mv | 10.1021/jf303004n |
| format | Article |
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Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of BRP induces MCF-7 cell apoptosis through, at least in part, ER stress-related signaling.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf303004n</identifier><identifier>PMID: 23066995</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>apoptosis ; Apoptosis - drug effects ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; breast neoplasms ; Breast Neoplasms - pathology ; caffeic acid ; Cell Line, Tumor ; cell viability ; DNA fragmentation ; DNA Primers ; endoplasmic reticulum ; Endoplasmic Reticulum - metabolism ; ethanol ; Ethanol - chemistry ; Female ; Food industries ; Fundamental and applied biological sciences. Psychology ; honey bees ; Humans ; Oxidative Stress ; Polymerase Chain Reaction ; propolis ; Propolis - chemistry ; Propolis - pharmacology ; Transcription Factor CHOP - biosynthesis</subject><ispartof>Journal of agricultural and food chemistry, 2012-11, Vol.60 (44), p.11065-11070</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a435t-f7db407afde0d75009d3f5a13d29cd857e713dffbb152fd6d91e842d823af7e53</citedby><cites>FETCH-LOGICAL-a435t-f7db407afde0d75009d3f5a13d29cd857e713dffbb152fd6d91e842d823af7e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf303004n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf303004n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27080,27928,27929,56742,56792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26620565$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23066995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamiya, Tetsuro</creatorcontrib><creatorcontrib>Nishihara, Hiroko</creatorcontrib><creatorcontrib>Hara, Hirokazu</creatorcontrib><creatorcontrib>Adachi, Tetsuo</creatorcontrib><title>Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF‑7 Cells through Endoplasmic Reticulum Stress</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of BRP induces MCF-7 cell apoptosis through, at least in part, ER stress-related signaling.</description><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>breast neoplasms</subject><subject>Breast Neoplasms - pathology</subject><subject>caffeic acid</subject><subject>Cell Line, Tumor</subject><subject>cell viability</subject><subject>DNA fragmentation</subject><subject>DNA Primers</subject><subject>endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>ethanol</subject><subject>Ethanol - chemistry</subject><subject>Female</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>honey bees</subject><subject>Humans</subject><subject>Oxidative Stress</subject><subject>Polymerase Chain Reaction</subject><subject>propolis</subject><subject>Propolis - chemistry</subject><subject>Propolis - pharmacology</subject><subject>Transcription Factor CHOP - biosynthesis</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0Mtu1DAUBmALgehQWPAC4A1SWQSO4ziXZTua0kpFIErX0RlfOhklcfBxJOiKDQ_AK_IkeDRDu2Fly_7065yfsZcC3gnIxfutkyABivERWwiVQ6aEqB-zBaTPrFalOGLPiLYAUKsKnrKjXEJZNo1asF-ruMHR93z1PQbUkXvHzwLedX2HI_9iDf8c_OT7jvjlaGZtiZ9Ofoqe0ks38ot5SO4sWKTIlzhqG_jH5fmfn78rvrR9Tzxugp9vN3w1Gj_1SEOnU27s9NzPA7-OwRI9Z08c9mRfHM5jdnO--rq8yK4-fbhcnl5lWEgVM1eZdQEVOmPBVAqgMdIpFNLkjTZpN1ulu3PrdWrBmdI0wtZFbupcoqusksfsZJ87Bf9tthTboSOdxsTR-plaIQpZl5UsdvTtnurgiYJ17RS6AcOPVkC7a729bz3ZV4fYeT1Ycy__1ZzAmwNA0ti7kIrq6MGVZQ6q3LnXe-fQt3gbkrm5zkEUAEI0UtYPSaip3fo5jKmv_4z0F7YFoN8</recordid><startdate>20121107</startdate><enddate>20121107</enddate><creator>Kamiya, Tetsuro</creator><creator>Nishihara, Hiroko</creator><creator>Hara, Hirokazu</creator><creator>Adachi, Tetsuo</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20121107</creationdate><title>Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF‑7 Cells through Endoplasmic Reticulum Stress</title><author>Kamiya, Tetsuro ; Nishihara, Hiroko ; Hara, Hirokazu ; Adachi, Tetsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a435t-f7db407afde0d75009d3f5a13d29cd857e713dffbb152fd6d91e842d823af7e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>breast neoplasms</topic><topic>Breast Neoplasms - pathology</topic><topic>caffeic acid</topic><topic>Cell Line, Tumor</topic><topic>cell viability</topic><topic>DNA fragmentation</topic><topic>DNA Primers</topic><topic>endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>ethanol</topic><topic>Ethanol - chemistry</topic><topic>Female</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>honey bees</topic><topic>Humans</topic><topic>Oxidative Stress</topic><topic>Polymerase Chain Reaction</topic><topic>propolis</topic><topic>Propolis - chemistry</topic><topic>Propolis - pharmacology</topic><topic>Transcription Factor CHOP - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamiya, Tetsuro</creatorcontrib><creatorcontrib>Nishihara, Hiroko</creatorcontrib><creatorcontrib>Hara, Hirokazu</creatorcontrib><creatorcontrib>Adachi, Tetsuo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamiya, Tetsuro</au><au>Nishihara, Hiroko</au><au>Hara, Hirokazu</au><au>Adachi, Tetsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF‑7 Cells through Endoplasmic Reticulum Stress</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2012-11-07</date><risdate>2012</risdate><volume>60</volume><issue>44</issue><spage>11065</spage><epage>11070</epage><pages>11065-11070</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Propolis, a natural product collected from plants by honey bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is known to induce apoptosis through the induction of CCAAT/enhancer-binding protein homologous protein (CHOP). Here, we investigated whether ethanol extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis, mitochondrial dysfunction, and ER stress in human breast cancer MCF-7 cells and human fibroblasts. Among several ethanol extracts of propolis and CAPE, Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through the induction of mitochondrial dysfunction, caspase-3 activity, and DNA fragmentation but did not affect those of fibroblasts. Moreover, treatment with BRP significantly induced CHOP expression in MCF-7 cells compared to fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an ethanol extract of BRP induces MCF-7 cell apoptosis through, at least in part, ER stress-related signaling.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23066995</pmid><doi>10.1021/jf303004n</doi><tpages>6</tpages></addata></record> |
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| subjects | apoptosis Apoptosis - drug effects Base Sequence Biological and medical sciences Blotting, Western breast neoplasms Breast Neoplasms - pathology caffeic acid Cell Line, Tumor cell viability DNA fragmentation DNA Primers endoplasmic reticulum Endoplasmic Reticulum - metabolism ethanol Ethanol - chemistry Female Food industries Fundamental and applied biological sciences. Psychology honey bees Humans Oxidative Stress Polymerase Chain Reaction propolis Propolis - chemistry Propolis - pharmacology Transcription Factor CHOP - biosynthesis |
| title | Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in Human Breast Cancer MCF‑7 Cells through Endoplasmic Reticulum Stress |
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