Analysis of the phospholipase C-δ1 pleckstrin homology domain using native polyacrylamide gel electrophoresis

The phospholipase C (PLC)-δ1 pleckstrin homology (PH) domain has a characteristic short α-helix (α2) from residues 82 to 87. The contributions of the α2-helix toward the inositol 1,4,5-trisphosphate (IP3) binding activity and thermal stability of the PLC-δ1 PH domain were investigated using native p...

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Veröffentlicht in:	Analytical biochemistry 2012-12, Vol.431 (2), p.106-114
Hauptverfasser:	Tanio, Michikazu, Nishimura, Katsuyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Humans Inositol 1,4,5-Trisphosphate - chemistry Kinetics Ligand affinity Ligands Native PAGE Native Polyacrylamide Gel Electrophoresis Phospholipase C delta - analysis Phospholipase C delta - chemistry Phospholipase C-δ1 Pleckstrin homology domain Protein Binding Protein Stability Protein Structure, Secondary Protein Structure, Tertiary Rats Thermal stability
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