An efficient method for enzyme immobilization evidenced by atomic force microscopy

Immobilization of proteins in a functionally active form and proper orientation is fundamental for effective surface-based protein analysis. A new method is presented for the controlled and oriented immobilization of ordered monolayers of enzymes whose interaction site had been protected using the p...

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Veröffentlicht in:	Protein engineering, design and selection design and selection, 2012-11, Vol.25 (11), p.715-723
Hauptverfasser:	Marcuello, C., de Miguel, R., Gómez-Moreno, C., Martínez-Júlvez, M., Lostao, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aluminum Silicates - chemistry Anabaena - chemistry Anabaena - enzymology Anabaena - metabolism Bacterial Proteins - chemistry Bacterial Proteins - metabolism Enzyme Assays Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Ferredoxin-NADP Reductase - chemistry Ferredoxin-NADP Reductase - metabolism Ferredoxins - chemistry Ferredoxins - metabolism Microscopy, Atomic Force Protein Interaction Mapping
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