Biosensor based on atemoya peroxidase immobilised on modified nanoclay for glyphosate biomonitoring
A biosensor based on atemoya peroxidase immobilised on modified nanoclay was developed for the determination of glyphosate by the enzyme inhibition method. The inhibitor effect of the biocide results in a decrease in the current response of the hydroquinone that was used as a phenolic substrate to o...
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| Veröffentlicht in: | Talanta (Oxford) 2012-08, Vol.98, p.130-136 |
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| creator | Oliveira, Grasielli C. Moccelini, Sally K. Castilho, Marilza Terezo, Ailton J. Possavatz, Juliana Magalhães, Márcia R.L. Dores, Eliana F.G.C. |
| description | A biosensor based on atemoya peroxidase immobilised on modified nanoclay was developed for the determination of glyphosate by the enzyme inhibition method. The inhibitor effect of the biocide results in a decrease in the current response of the hydroquinone that was used as a phenolic substrate to obtain the base signal. The biosensor was constructed using graphite powder, multiwalled carbon nanotubes, peroxidase immobilised on nanoclay and mineral oil. Square-wave voltammetry was utilised for the optimisation and application of the biosensor, and several parameters were investigated to determine the optimum experimental conditions. The best performance was obtained using a 0.1molL−1 phosphate buffer solution (pH 7.0), 1.9×10−4molL−1 hydrogen peroxide, a frequency of 30Hz, a pulse amplitude of 50mV and a scan increment of 4mV. The glyphosate concentration response was linear between 0.10 and 4.55mgL−1 with a detection limit of 30μgL−1. The average recovery of glyphosate from spiked water samples ranged from 94.9 to 108.9%. The biosensor remained stable for a period of eight weeks.
► A novel atemoya biosensor for glyphosate determination was developed. ► The biosensor was based on the inhibitor effect of the pesticide on enzymatic activity. ► The peroxidase enzyme was immobilised on nanoclay based on montmorillonite modified. ► Multi-walled carbon nanotubes were incorporated into a carbon paste electrode. ► Square-wave voltammetry was used for the optimisation and application of the biosensor. |
| doi_str_mv | 10.1016/j.talanta.2012.06.059 |
| format | Article |
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► A novel atemoya biosensor for glyphosate determination was developed. ► The biosensor was based on the inhibitor effect of the pesticide on enzymatic activity. ► The peroxidase enzyme was immobilised on nanoclay based on montmorillonite modified. ► Multi-walled carbon nanotubes were incorporated into a carbon paste electrode. ► Square-wave voltammetry was used for the optimisation and application of the biosensor.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2012.06.059</identifier><identifier>PMID: 22939138</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Aluminum Silicates ; Analytical chemistry ; Annonaceae - chemistry ; Annonaceae - enzymology ; Atemoya ; Biological and medical sciences ; Biosensing Techniques ; Biosensor ; Biosensors ; Biotechnology ; Buffers ; Carbon nanotubes ; Chemistry ; Clay ; Electrochemical methods ; Electrochemical Techniques ; Electrodes ; Enzymes, Immobilized - chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Glycine - analogs & derivatives ; Glycine - analysis ; Glyphosate ; Graphite ; Herbicides - analysis ; Hydrogen-Ion Concentration ; Hydroquinones - chemistry ; Limit of Detection ; Methods. Procedures. Technologies ; Mineral Oil ; Nanoclay ; Nanotubes, Carbon ; Peroxidase - chemistry ; Phosphates ; Plant Proteins - chemistry ; Various methods and equipments ; Water Pollutants, Chemical - analysis</subject><ispartof>Talanta (Oxford), 2012-08, Vol.98, p.130-136</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-a61f2d5d5af0e39f0aa320e04c7fd3947cc84589bf9d2c81cda746d2431cd6cc3</citedby><cites>FETCH-LOGICAL-c475t-a61f2d5d5af0e39f0aa320e04c7fd3947cc84589bf9d2c81cda746d2431cd6cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2012.06.059$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26374973$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22939138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Grasielli C.</creatorcontrib><creatorcontrib>Moccelini, Sally K.</creatorcontrib><creatorcontrib>Castilho, Marilza</creatorcontrib><creatorcontrib>Terezo, Ailton J.</creatorcontrib><creatorcontrib>Possavatz, Juliana</creatorcontrib><creatorcontrib>Magalhães, Márcia R.L.</creatorcontrib><creatorcontrib>Dores, Eliana F.G.C.</creatorcontrib><title>Biosensor based on atemoya peroxidase immobilised on modified nanoclay for glyphosate biomonitoring</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A biosensor based on atemoya peroxidase immobilised on modified nanoclay was developed for the determination of glyphosate by the enzyme inhibition method. The inhibitor effect of the biocide results in a decrease in the current response of the hydroquinone that was used as a phenolic substrate to obtain the base signal. The biosensor was constructed using graphite powder, multiwalled carbon nanotubes, peroxidase immobilised on nanoclay and mineral oil. Square-wave voltammetry was utilised for the optimisation and application of the biosensor, and several parameters were investigated to determine the optimum experimental conditions. The best performance was obtained using a 0.1molL−1 phosphate buffer solution (pH 7.0), 1.9×10−4molL−1 hydrogen peroxide, a frequency of 30Hz, a pulse amplitude of 50mV and a scan increment of 4mV. The glyphosate concentration response was linear between 0.10 and 4.55mgL−1 with a detection limit of 30μgL−1. The average recovery of glyphosate from spiked water samples ranged from 94.9 to 108.9%. The biosensor remained stable for a period of eight weeks.
► A novel atemoya biosensor for glyphosate determination was developed. ► The biosensor was based on the inhibitor effect of the pesticide on enzymatic activity. ► The peroxidase enzyme was immobilised on nanoclay based on montmorillonite modified. ► Multi-walled carbon nanotubes were incorporated into a carbon paste electrode. ► Square-wave voltammetry was used for the optimisation and application of the biosensor.</description><subject>Aluminum Silicates</subject><subject>Analytical chemistry</subject><subject>Annonaceae - chemistry</subject><subject>Annonaceae - enzymology</subject><subject>Atemoya</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Buffers</subject><subject>Carbon nanotubes</subject><subject>Chemistry</subject><subject>Clay</subject><subject>Electrochemical methods</subject><subject>Electrochemical Techniques</subject><subject>Electrodes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - analysis</subject><subject>Glyphosate</subject><subject>Graphite</subject><subject>Herbicides - analysis</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroquinones - chemistry</subject><subject>Limit of Detection</subject><subject>Methods. Procedures. Technologies</subject><subject>Mineral Oil</subject><subject>Nanoclay</subject><subject>Nanotubes, Carbon</subject><subject>Peroxidase - chemistry</subject><subject>Phosphates</subject><subject>Plant Proteins - chemistry</subject><subject>Various methods and equipments</subject><subject>Water Pollutants, Chemical - analysis</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi1ERZfCTwDlgtRL0rGd2PEJQVU-pEpc4GxN_FG8SuzFziL23-NqAxw5zWj0vDOjh5BXFDoKVNzsuxVnjCt2DCjrQHQwqCdkR0fJWz5I_pTsALhqFe3hkjwvZQ8AjAN_Ri4ZU1xRPu6IeR9ScbGk3ExYnG1SbHB1Szphc3A5_Qq2jpuwLGkKc9iIJdngQ-0jxmRmPDW-LniYT4fvqdR4M4W0pBjWlEN8eEEuPM7FvdzqFfn24e7r7af2_svHz7fv7lvTy2FtUVDP7GAH9OC48oDIGTjojfSWq14aM_bDqCavLDMjNRZlLyzreW2FMfyKXJ_3HnL6cXRl1Usoxs1Vk0vHoinlYhBCSlbR4YyanErJzutDDgvmk6agH_3qvd786ke_GoSufmvu9XbiOC3O_k39EVqBNxuAxeDsM0YTyj9OcNkrySv39sy5KuRncFkXE1w0zobszKptCv955Tfkrp4o</recordid><startdate>20120830</startdate><enddate>20120830</enddate><creator>Oliveira, Grasielli C.</creator><creator>Moccelini, Sally K.</creator><creator>Castilho, Marilza</creator><creator>Terezo, Ailton J.</creator><creator>Possavatz, Juliana</creator><creator>Magalhães, Márcia R.L.</creator><creator>Dores, Eliana F.G.C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120830</creationdate><title>Biosensor based on atemoya peroxidase immobilised on modified nanoclay for glyphosate biomonitoring</title><author>Oliveira, Grasielli C. ; Moccelini, Sally K. ; Castilho, Marilza ; Terezo, Ailton J. ; Possavatz, Juliana ; Magalhães, Márcia R.L. ; Dores, Eliana F.G.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-a61f2d5d5af0e39f0aa320e04c7fd3947cc84589bf9d2c81cda746d2431cd6cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aluminum Silicates</topic><topic>Analytical chemistry</topic><topic>Annonaceae - chemistry</topic><topic>Annonaceae - enzymology</topic><topic>Atemoya</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Buffers</topic><topic>Carbon nanotubes</topic><topic>Chemistry</topic><topic>Clay</topic><topic>Electrochemical methods</topic><topic>Electrochemical Techniques</topic><topic>Electrodes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - analysis</topic><topic>Glyphosate</topic><topic>Graphite</topic><topic>Herbicides - analysis</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroquinones - chemistry</topic><topic>Limit of Detection</topic><topic>Methods. Procedures. Technologies</topic><topic>Mineral Oil</topic><topic>Nanoclay</topic><topic>Nanotubes, Carbon</topic><topic>Peroxidase - chemistry</topic><topic>Phosphates</topic><topic>Plant Proteins - chemistry</topic><topic>Various methods and equipments</topic><topic>Water Pollutants, Chemical - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Grasielli C.</creatorcontrib><creatorcontrib>Moccelini, Sally K.</creatorcontrib><creatorcontrib>Castilho, Marilza</creatorcontrib><creatorcontrib>Terezo, Ailton J.</creatorcontrib><creatorcontrib>Possavatz, Juliana</creatorcontrib><creatorcontrib>Magalhães, Márcia R.L.</creatorcontrib><creatorcontrib>Dores, Eliana F.G.C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Grasielli C.</au><au>Moccelini, Sally K.</au><au>Castilho, Marilza</au><au>Terezo, Ailton J.</au><au>Possavatz, Juliana</au><au>Magalhães, Márcia R.L.</au><au>Dores, Eliana F.G.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosensor based on atemoya peroxidase immobilised on modified nanoclay for glyphosate biomonitoring</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2012-08-30</date><risdate>2012</risdate><volume>98</volume><spage>130</spage><epage>136</epage><pages>130-136</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>A biosensor based on atemoya peroxidase immobilised on modified nanoclay was developed for the determination of glyphosate by the enzyme inhibition method. The inhibitor effect of the biocide results in a decrease in the current response of the hydroquinone that was used as a phenolic substrate to obtain the base signal. The biosensor was constructed using graphite powder, multiwalled carbon nanotubes, peroxidase immobilised on nanoclay and mineral oil. Square-wave voltammetry was utilised for the optimisation and application of the biosensor, and several parameters were investigated to determine the optimum experimental conditions. The best performance was obtained using a 0.1molL−1 phosphate buffer solution (pH 7.0), 1.9×10−4molL−1 hydrogen peroxide, a frequency of 30Hz, a pulse amplitude of 50mV and a scan increment of 4mV. The glyphosate concentration response was linear between 0.10 and 4.55mgL−1 with a detection limit of 30μgL−1. The average recovery of glyphosate from spiked water samples ranged from 94.9 to 108.9%. The biosensor remained stable for a period of eight weeks.
► A novel atemoya biosensor for glyphosate determination was developed. ► The biosensor was based on the inhibitor effect of the pesticide on enzymatic activity. ► The peroxidase enzyme was immobilised on nanoclay based on montmorillonite modified. ► Multi-walled carbon nanotubes were incorporated into a carbon paste electrode. ► Square-wave voltammetry was used for the optimisation and application of the biosensor.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22939138</pmid><doi>10.1016/j.talanta.2012.06.059</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aluminum Silicates Analytical chemistry Annonaceae - chemistry Annonaceae - enzymology Atemoya Biological and medical sciences Biosensing Techniques Biosensor Biosensors Biotechnology Buffers Carbon nanotubes Chemistry Clay Electrochemical methods Electrochemical Techniques Electrodes Enzymes, Immobilized - chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Glycine - analogs & derivatives Glycine - analysis Glyphosate Graphite Herbicides - analysis Hydrogen-Ion Concentration Hydroquinones - chemistry Limit of Detection Methods. Procedures. Technologies Mineral Oil Nanoclay Nanotubes, Carbon Peroxidase - chemistry Phosphates Plant Proteins - chemistry Various methods and equipments Water Pollutants, Chemical - analysis |
| title | Biosensor based on atemoya peroxidase immobilised on modified nanoclay for glyphosate biomonitoring |
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