Development of an enzymeless electroanalytical method for the indirect detection of creatinine in urine samples
The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has...
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| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2012-10, Vol.173, p.847-851 |
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| container_title | Sensors and actuators. B, Chemical |
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| creator | de Araújo, William R. Salles, Maiara O. Paixão, Thiago R.L.C. |
| description | The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80μmolL−1, with a detection limit of 380nmolL−1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level). |
| doi_str_mv | 10.1016/j.snb.2012.07.114 |
| format | Article |
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This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80μmolL−1, with a detection limit of 380nmolL−1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level).</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2012.07.114</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>ascorbic acid ; carbon ; Confidence intervals ; Creatinine ; detection limit ; Differential pulse technique ; electrochemistry ; Electrodes ; Glassy carbon ; glucose ; humans ; Indirect quantification ; Jaffe's reaction ; monitoring ; Non-modified electrodes ; Picrates ; Picric acid ; Reduction ; Selectivity ; spectrophotometers ; temperature ; Uric acid ; Urine</subject><ispartof>Sensors and actuators. 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B, Chemical</title><description>The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80μmolL−1, with a detection limit of 380nmolL−1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level).</description><subject>ascorbic acid</subject><subject>carbon</subject><subject>Confidence intervals</subject><subject>Creatinine</subject><subject>detection limit</subject><subject>Differential pulse technique</subject><subject>electrochemistry</subject><subject>Electrodes</subject><subject>Glassy carbon</subject><subject>glucose</subject><subject>humans</subject><subject>Indirect quantification</subject><subject>Jaffe's reaction</subject><subject>monitoring</subject><subject>Non-modified electrodes</subject><subject>Picrates</subject><subject>Picric acid</subject><subject>Reduction</subject><subject>Selectivity</subject><subject>spectrophotometers</subject><subject>temperature</subject><subject>Uric acid</subject><subject>Urine</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kMFu1DAQhi0EEkvpA3DCRy4J4zi2E3FChRakShxoz5ZjT6hXib3Y3krL0-MonLnMzOH7P2l-Qt4xaBkw-fHY5jC1HbCuBdUy1r8gBzYo3nBQ6iU5wNiJpgcQr8mbnI8A0HMJBxK_4DMu8bRiKDTO1ASK4c9lxQVzpnXakqIJZrkUb81CVyxP0dE5JlqekPrgfKoMdVjq8jFsEpvQFB982AB6TtuRzXqqzrfk1WyWjNf_9hV5vP36cPOtuf9x9_3m831juehLM3UTl4pz2YtOKhwHYwZebyd7nO1kp8F10gkHRsh5GHBwdpR8NPNYEaaQX5EPu_eU4u8z5qJXny0uiwkYz1kzVt1KSCUrynbUpphzwlmfkl9NumgGeitXH3UtV2_lalA12tfM-z0zm6jNr-SzfvxZAQHAmBh7XolPO4H1y2ePSWfrMVjcC9Mu-v_4_wJMPY15</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>de Araújo, William R.</creator><creator>Salles, Maiara O.</creator><creator>Paixão, Thiago R.L.C.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20121001</creationdate><title>Development of an enzymeless electroanalytical method for the indirect detection of creatinine in urine samples</title><author>de Araújo, William R. ; Salles, Maiara O. ; Paixão, Thiago R.L.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-b2b36733645267e98aa83452d64efcbcb8d26d5d0a56f88e8dc9639af92d617e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>ascorbic acid</topic><topic>carbon</topic><topic>Confidence intervals</topic><topic>Creatinine</topic><topic>detection limit</topic><topic>Differential pulse technique</topic><topic>electrochemistry</topic><topic>Electrodes</topic><topic>Glassy carbon</topic><topic>glucose</topic><topic>humans</topic><topic>Indirect quantification</topic><topic>Jaffe's reaction</topic><topic>monitoring</topic><topic>Non-modified electrodes</topic><topic>Picrates</topic><topic>Picric acid</topic><topic>Reduction</topic><topic>Selectivity</topic><topic>spectrophotometers</topic><topic>temperature</topic><topic>Uric acid</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Araújo, William R.</creatorcontrib><creatorcontrib>Salles, Maiara O.</creatorcontrib><creatorcontrib>Paixão, Thiago R.L.C.</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Araújo, William R.</au><au>Salles, Maiara O.</au><au>Paixão, Thiago R.L.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an enzymeless electroanalytical method for the indirect detection of creatinine in urine samples</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2012-10-01</date><risdate>2012</risdate><volume>173</volume><spage>847</spage><epage>851</epage><pages>847-851</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>The aim of this study is to develop a new enzymeless electroanalytical method for the indirect quantification of creatinine from urine sample. This method is based on the electrochemical monitoring of picrate anion reduction at a glassy carbon electrode in an alkaline medium before and after it has reacted with creatinine (Jaffe's reaction). By using the differential pulse voltammetry technique under the optimum experimental conditions (step potential, amplitude potential, reaction time, and temperature), a linear analytical curve was obtained for concentrations of creatinine ranging from 1 to 80μmolL−1, with a detection limit of 380nmolL−1. This proposed method was used to measure creatinine in human urine without the interference of most common organic species normally present in biological fluids (e.g., uric acid, ascorbic acid, glucose, and phosphocreatinine). The results obtained using urine samples were highly similar to the results obtained using the reference spectrophotometric method (at a 95% confidence level).</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2012.07.114</doi><tpages>5</tpages></addata></record> |
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| ispartof | Sensors and actuators. B, Chemical, 2012-10, Vol.173, p.847-851 |
| issn | 0925-4005 1873-3077 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | ascorbic acid carbon Confidence intervals Creatinine detection limit Differential pulse technique electrochemistry Electrodes Glassy carbon glucose humans Indirect quantification Jaffe's reaction monitoring Non-modified electrodes Picrates Picric acid Reduction Selectivity spectrophotometers temperature Uric acid Urine |
| title | Development of an enzymeless electroanalytical method for the indirect detection of creatinine in urine samples |
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