Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes

A novel molecular assay for Clostridium difficile was developed using Linear-After-The-Exponential polymerase chain reaction (LATE-PCR). Single-stranded DNA products generated by LATE-PCR were detected and distinguished by hybridization to fluorescent mismatch-tolerant probes, as the temperature was...

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Veröffentlicht in:	Journal of microbiological methods 2012-11, Vol.91 (2), p.269-275
Hauptverfasser:	Pierce, Kenneth E., Khan, Huma, Mistry, Rohit, Goldenberg, Simon D., French, Gary L., Wangh, Lawrence J.
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP Ribose Transferases - genetics Bacterial Proteins - genetics Bacterial Toxins - genetics Biological and medical sciences Clostridium difficile Clostridium difficile - classification Clostridium difficile - genetics color DNA, Bacterial - genetics Enterotoxins - genetics fluorescence fluorescent dyes Fundamental and applied biological sciences. Psychology genes Hybridization probes Infectious disease diagnosis LATE-PCR Microbiology Molecular Typing - methods Multiplex PCR Nucleic Acid Hybridization Oligonucleotide Probes - genetics polymerase chain reaction Polymerase Chain Reaction - methods rapid methods Repressor Proteins - genetics sequence deletion single-stranded DNA stop codon Temperature virulence
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