Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification
Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ri...
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| Veröffentlicht in: | Japanese Journal of Infectious Diseases 2012, Vol.65(4), pp.306-311 |
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| container_title | Japanese Journal of Infectious Diseases |
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| creator | Rudeeaneksin, Janisara Bunchoo, Supranee Srisungngam, Sopa Sawanpanyalert, Pathom Chamnangrom, Sawet Kamolwat, Atipa Thanasripakdeekul, Porntip Taniguchi, Tooru Nakajima, Chie Suzuki, Yasuhiko Phetsuksiri, Benjawan |
| description | Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex. |
| doi_str_mv | 10.7883/yoken.65.306 |
| format | Article |
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Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex.</description><identifier>ISSN: 1344-6304</identifier><identifier>EISSN: 1884-2836</identifier><identifier>DOI: 10.7883/yoken.65.306</identifier><identifier>PMID: 22814152</identifier><language>eng</language><publisher>Japan: National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</publisher><subject>Humans ; Indexing in process ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - growth & development ; Mycobacterium tuberculosis - isolation & purification ; Nucleic Acid Amplification Techniques - methods ; RNA, Ribosomal, 16S - genetics ; Sensitivity and Specificity ; Tuberculosis - diagnosis</subject><ispartof>Japanese Journal of Infectious Diseases, 2012, Vol.65(4), pp.306-311</ispartof><rights>Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-f0be9cd6f8e051117dca1e51ebd72ed20d28a80cfbf348ec37cd7f3fcacdff1a3</citedby><cites>FETCH-LOGICAL-c592t-f0be9cd6f8e051117dca1e51ebd72ed20d28a80cfbf348ec37cd7f3fcacdff1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,1879,4012,27906,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22814152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rudeeaneksin, Janisara</creatorcontrib><creatorcontrib>Bunchoo, Supranee</creatorcontrib><creatorcontrib>Srisungngam, Sopa</creatorcontrib><creatorcontrib>Sawanpanyalert, Pathom</creatorcontrib><creatorcontrib>Chamnangrom, Sawet</creatorcontrib><creatorcontrib>Kamolwat, Atipa</creatorcontrib><creatorcontrib>Thanasripakdeekul, Porntip</creatorcontrib><creatorcontrib>Taniguchi, Tooru</creatorcontrib><creatorcontrib>Nakajima, Chie</creatorcontrib><creatorcontrib>Suzuki, Yasuhiko</creatorcontrib><creatorcontrib>Phetsuksiri, Benjawan</creatorcontrib><title>Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification</title><title>Japanese Journal of Infectious Diseases</title><addtitle>Jpn J Infect Dis</addtitle><description>Definitive diagnosis of tuberculosis (TB) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. These results indicate that TB-LAMP in combination with BACTEC MGIT 960 is a specific, reliable, and technically feasible method for rapid and accurate identification of M. tuberculosis complex.</description><subject>Humans</subject><subject>Indexing in process</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Tuberculosis - diagnosis</subject><issn>1344-6304</issn><issn>1884-2836</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtv2zAURomiQfPq1jng2CFy-RAleiocIQ8DNgIE7ixQ5GXDRBJVkho85p9HiR1n7HQvcA_Od4EPoR-UzEop-a-tf4Z-VogZJ8UXdEKlzDMmefF12nmeZwUn-TE6jfGJECYEJd_QMWOS5lSwE_TyoAZn8NJAn5x1WiXne-wtXm-1b5ROENzY4TQ2EPTY-ugidj2-WlSb6wqvb5ebeUFwNbZpDBBxs8XLPrvzYwS88n7I1mDepDBFRJ8eIXSqxYtuaA9h5-jIqjbC9_08Q39urjfVXba6v11Wi1WmxZylzJIG5toUVgIRlNLSaEVBUGhMycAwYphUkmjbWJ5L0LzUprTcaqWNtVTxM_Rz5x2C_zdCTHXnooa2VT1M_9aUMsE4n4vi_yhhJS-FLNmEXu5QHXyMAWw9BNepsJ2g-q2f-r2fuhD11M-EX-zNY9OBOcAfhUzA7x3wFJP6CwdAheR0C5-2fK88XPSjCjX0_BWFNKWt</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Rudeeaneksin, Janisara</creator><creator>Bunchoo, Supranee</creator><creator>Srisungngam, Sopa</creator><creator>Sawanpanyalert, Pathom</creator><creator>Chamnangrom, Sawet</creator><creator>Kamolwat, Atipa</creator><creator>Thanasripakdeekul, Porntip</creator><creator>Taniguchi, Tooru</creator><creator>Nakajima, Chie</creator><creator>Suzuki, Yasuhiko</creator><creator>Phetsuksiri, Benjawan</creator><general>National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>2012</creationdate><title>Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification</title><author>Rudeeaneksin, Janisara ; 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Simple loop-mediated isothermal amplification (LAMP) specific for Mycobacterium tuberculosis complex, targeting the M. tuberculosis 16S ribosomal RNA gene, termed TB-LAMP, was evaluated as an alternative for rapid culture confirmation. TB-LAMP was assessed for its ability to detect M. tuberculosis complex in BACTEC MGIT 960-positive cultures. Of the 103 cultures evaluated, 100 were identified to contain M. tuberculosis complex by TB-LAMP and had concordant results with standard biochemical tests of niacin accumulation, nitrate reductase, lack of heat-stable catalase, and susceptibility to para-nitrobenzoic acid. 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| subjects | Humans Indexing in process Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - isolation & purification Nucleic Acid Amplification Techniques - methods RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Tuberculosis - diagnosis |
| title | Rapid Identification of Mycobacterium tuberculosis in BACTEC MGIT960 Cultures by In-House Loop-Medicated Isothermal Amplification |
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