Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil
A bacterial strain named CB4, with highly effective glyphosate degradation capability, was isolated from soil after enrichment. On the basis of the Biolog omniLog identification system (Biolog) and 16S ribosomal RNA (rRNA) gene sequencing methods, strain CB4 was identified as Bacillus cereus. Furthe...
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| Veröffentlicht in: | Journal of general and applied microbiology 2012, Vol.58(4), pp.263-271 |
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| creator | Fan, Jieyu Yang, Guoxia Zhao, Haoyu Shi, Guanying Geng, Yucong Hou, Taiping Tao, Ke |
| description | A bacterial strain named CB4, with highly effective glyphosate degradation capability, was isolated from soil after enrichment. On the basis of the Biolog omniLog identification system (Biolog) and 16S ribosomal RNA (rRNA) gene sequencing methods, strain CB4 was identified as Bacillus cereus. Further experiments were carried out to optimize the growth of strain CB4 and the glyphosate degradation activity by high performance liquid chromatography (HPLC). The optimal conditions were found as follows: initial pH 6.0, incubation temperature 35°C, glyphosate concentration 6 g L−1, inoculation amount 5% and incubation time 5 days. Under the optimal conditions, stain CB4 utilized 94.47% of glyphosate. This is the first report on B. cereus with a capacity to utilize herbicide glyphosate, and it can degrade glyphosate concentrations up to 12 g L−1. Metabolization of glyphosate by strain B. cereus CB4 was studied. Results indicated that two concurrent pathways were capable of degrading glyphosate to AMPA, glyoxylate, sarcosine, glycine and formaldehyde as products. Glyphosate breakdown in B. cereus CB4 was achieved by the C-P lyase activity and the glyphosate oxidoreductase activity. |
| doi_str_mv | 10.2323/jgam.58.263 |
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On the basis of the Biolog omniLog identification system (Biolog) and 16S ribosomal RNA (rRNA) gene sequencing methods, strain CB4 was identified as Bacillus cereus. Further experiments were carried out to optimize the growth of strain CB4 and the glyphosate degradation activity by high performance liquid chromatography (HPLC). The optimal conditions were found as follows: initial pH 6.0, incubation temperature 35°C, glyphosate concentration 6 g L−1, inoculation amount 5% and incubation time 5 days. Under the optimal conditions, stain CB4 utilized 94.47% of glyphosate. This is the first report on B. cereus with a capacity to utilize herbicide glyphosate, and it can degrade glyphosate concentrations up to 12 g L−1. Metabolization of glyphosate by strain B. cereus CB4 was studied. Results indicated that two concurrent pathways were capable of degrading glyphosate to AMPA, glyoxylate, sarcosine, glycine and formaldehyde as products. Glyphosate breakdown in B. cereus CB4 was achieved by the C-P lyase activity and the glyphosate oxidoreductase activity.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.58.263</identifier><identifier>PMID: 22990486</identifier><identifier>CODEN: JGAMA9</identifier><language>eng</language><publisher>Tokyo: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>Bacillus cereus ; Bacillus cereus - classification ; Bacillus cereus - genetics ; Bacillus cereus - isolation & purification ; Bacillus cereus - metabolism ; Bacterial Typing Techniques ; biodegradation ; Biological and medical sciences ; Biotransformation ; Chromatography, High Pressure Liquid ; Cluster Analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Glycine - analogs & derivatives ; Glycine - metabolism ; Glyphosate ; herbicide ; Herbicides - metabolism ; Hydrogen-Ion Concentration ; metabolism ; Microbiology ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil Microbiology ; Temperature</subject><ispartof>The Journal of General and Applied Microbiology, 2012, Vol.58(4), pp.263-271</ispartof><rights>2012, The Microbiology Research Foundation</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-bf9d7d0660efee1f7eed2fe782738773adb24ea9b9dbfd7fb198680c6fa52c3c3</citedby><cites>FETCH-LOGICAL-c534t-bf9d7d0660efee1f7eed2fe782738773adb24ea9b9dbfd7fb198680c6fa52c3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,4009,27901,27902,27903</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26470894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22990486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fan, Jieyu</creatorcontrib><creatorcontrib>Yang, Guoxia</creatorcontrib><creatorcontrib>Zhao, Haoyu</creatorcontrib><creatorcontrib>Shi, Guanying</creatorcontrib><creatorcontrib>Geng, Yucong</creatorcontrib><creatorcontrib>Hou, Taiping</creatorcontrib><creatorcontrib>Tao, Ke</creatorcontrib><title>Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil</title><title>Journal of general and applied microbiology</title><addtitle>J. Gen. Appl. Microbiol.</addtitle><description>A bacterial strain named CB4, with highly effective glyphosate degradation capability, was isolated from soil after enrichment. On the basis of the Biolog omniLog identification system (Biolog) and 16S ribosomal RNA (rRNA) gene sequencing methods, strain CB4 was identified as Bacillus cereus. Further experiments were carried out to optimize the growth of strain CB4 and the glyphosate degradation activity by high performance liquid chromatography (HPLC). The optimal conditions were found as follows: initial pH 6.0, incubation temperature 35°C, glyphosate concentration 6 g L−1, inoculation amount 5% and incubation time 5 days. Under the optimal conditions, stain CB4 utilized 94.47% of glyphosate. This is the first report on B. cereus with a capacity to utilize herbicide glyphosate, and it can degrade glyphosate concentrations up to 12 g L−1. Metabolization of glyphosate by strain B. cereus CB4 was studied. Results indicated that two concurrent pathways were capable of degrading glyphosate to AMPA, glyoxylate, sarcosine, glycine and formaldehyde as products. Glyphosate breakdown in B. cereus CB4 was achieved by the C-P lyase activity and the glyphosate oxidoreductase activity.</description><subject>Bacillus cereus</subject><subject>Bacillus cereus - classification</subject><subject>Bacillus cereus - genetics</subject><subject>Bacillus cereus - isolation & purification</subject><subject>Bacillus cereus - metabolism</subject><subject>Bacterial Typing Techniques</subject><subject>biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cluster Analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - metabolism</subject><subject>Glyphosate</subject><subject>herbicide</subject><subject>Herbicides - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Microbiology</subject><subject>Temperature</subject><issn>0022-1260</issn><issn>1349-8037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0UGL1DAUB_Agiju7evIuuQiC0zFN0ja9CO6w6sKCFz2H1-SlmyFtxqQ9rJ_ejh3H0yMvP96D_yPkTcl2XHDx8dDDsKvUjtfiGdmUQraFYqJ5TjaMcV6UvGZX5DrnA2Oi5kq-JFecty2Tqt6QdJ9jgMnHcUu9xXHyzpu_bwqjpeYREpgJk_-9NqOjQPvwdHyMGSYsLPYJrB972q1uHrb0FowPYc7UYMKl7G_llroUB5qjD6_ICwch4-tzvSE_v9z92H8rHr5_vd9_fihMJeRUdK61jWV1zdAhlq5BtNxho3gjVNMIsB2XCG3X2s7ZxnVlq2rFTO2g4kYYcUPer3OPKf6aMU968NlgCDBinLMuS15xIYSsFvphpSbFnBM6fUx-gPSkS6ZPIetTyLpSegl50W_Pg-duQHux_1JdwLszgGwguASj8fm_q2XDVCsX92l1hzxBjxcAafIm4GWpPG--fJzOonEUfwDIWp4h</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Fan, Jieyu</creator><creator>Yang, Guoxia</creator><creator>Zhao, Haoyu</creator><creator>Shi, Guanying</creator><creator>Geng, Yucong</creator><creator>Hou, Taiping</creator><creator>Tao, Ke</creator><general>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</general><general>Microbiology Research Foundation</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2012</creationdate><title>Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil</title><author>Fan, Jieyu ; Yang, Guoxia ; Zhao, Haoyu ; Shi, Guanying ; Geng, Yucong ; Hou, Taiping ; Tao, Ke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-bf9d7d0660efee1f7eed2fe782738773adb24ea9b9dbfd7fb198680c6fa52c3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Bacillus cereus</topic><topic>Bacillus cereus - classification</topic><topic>Bacillus cereus - genetics</topic><topic>Bacillus cereus - isolation & purification</topic><topic>Bacillus cereus - metabolism</topic><topic>Bacterial Typing Techniques</topic><topic>biodegradation</topic><topic>Biological and medical sciences</topic><topic>Biotransformation</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cluster Analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - metabolism</topic><topic>Glyphosate</topic><topic>herbicide</topic><topic>Herbicides - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>metabolism</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Soil Microbiology</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Jieyu</creatorcontrib><creatorcontrib>Yang, Guoxia</creatorcontrib><creatorcontrib>Zhao, Haoyu</creatorcontrib><creatorcontrib>Shi, Guanying</creatorcontrib><creatorcontrib>Geng, Yucong</creatorcontrib><creatorcontrib>Hou, Taiping</creatorcontrib><creatorcontrib>Tao, Ke</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Jieyu</au><au>Yang, Guoxia</au><au>Zhao, Haoyu</au><au>Shi, Guanying</au><au>Geng, Yucong</au><au>Hou, Taiping</au><au>Tao, Ke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>2012</date><risdate>2012</risdate><volume>58</volume><issue>4</issue><spage>263</spage><epage>271</epage><pages>263-271</pages><issn>0022-1260</issn><eissn>1349-8037</eissn><coden>JGAMA9</coden><abstract>A bacterial strain named CB4, with highly effective glyphosate degradation capability, was isolated from soil after enrichment. On the basis of the Biolog omniLog identification system (Biolog) and 16S ribosomal RNA (rRNA) gene sequencing methods, strain CB4 was identified as Bacillus cereus. Further experiments were carried out to optimize the growth of strain CB4 and the glyphosate degradation activity by high performance liquid chromatography (HPLC). The optimal conditions were found as follows: initial pH 6.0, incubation temperature 35°C, glyphosate concentration 6 g L−1, inoculation amount 5% and incubation time 5 days. Under the optimal conditions, stain CB4 utilized 94.47% of glyphosate. This is the first report on B. cereus with a capacity to utilize herbicide glyphosate, and it can degrade glyphosate concentrations up to 12 g L−1. Metabolization of glyphosate by strain B. cereus CB4 was studied. Results indicated that two concurrent pathways were capable of degrading glyphosate to AMPA, glyoxylate, sarcosine, glycine and formaldehyde as products. Glyphosate breakdown in B. cereus CB4 was achieved by the C-P lyase activity and the glyphosate oxidoreductase activity.</abstract><cop>Tokyo</cop><pub>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</pub><pmid>22990486</pmid><doi>10.2323/jgam.58.263</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus cereus Bacillus cereus - classification Bacillus cereus - genetics Bacillus cereus - isolation & purification Bacillus cereus - metabolism Bacterial Typing Techniques biodegradation Biological and medical sciences Biotransformation Chromatography, High Pressure Liquid Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Glycine - analogs & derivatives Glycine - metabolism Glyphosate herbicide Herbicides - metabolism Hydrogen-Ion Concentration metabolism Microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Soil Microbiology Temperature |
| title | Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil |
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