Routine use of duplex real-time PCR assays including a commercial internal control for molecular diagnosis of opportunistic DNA virus infections

► Improving the validity of laboratory-developed real-time PCRs for the detection/quantitation of DNA viruses. ► Use of the commercial Simplexa™ extraction and amplification control (SEAC) set. ► The SEAC showed high reproducibility and high stability. ► Procedure for validation of PCR results in qu...

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Veröffentlicht in:	Journal of virological methods 2012-10, Vol.185 (1), p.136-141
Hauptverfasser:	Burrel, Sonia, Fovet, Christelle, Brunet, Christel, Ovaguimian, Lydia, Hamm, Nathalie, Conan, Françoise, Kalkias, Laurence, Agut, Henri, Boutolleau, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenovirus Biological and medical sciences BK virus Data processing DNA DNA Virus Infections - diagnosis DNA Virus Infections - virology Epstein-Barr virus Extraction and amplification process monitoring Fundamental and applied biological sciences. Psychology genome Genomes herpes simplex Herpes simplex virus Human cytomegalovirus Human herpesvirus 3 Human herpesvirus 4 Human herpesvirus 5 Humans Infection Internal control Laboratory-developed PCR Microbiology Molecular Diagnostic Techniques - methods Molecular Diagnostic Techniques - standards monitoring Multiplex Polymerase Chain Reaction - methods Multiplex Polymerase Chain Reaction - standards Opportunistic Infections - diagnosis Opportunistic Infections - virology Polymerase chain reaction Quality assurance Quality control quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reference Standards Reproducibility of Results Shelf life Techniques used in virology Temperature Validity improvement Varicella-zoster virus Virology Virology - methods Virology - standards viruses
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