Nanoscale Mapping and Affinity Constant Measurement of Signal-Transducing Proteins by Atomic Force Microscopy

Atomic force microscope (AFM) was used to measure the interaction force between two signal-transducing proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Ras homologue enriched in brain (Rheb), and to analyze the binding of glyceraldehyde-3-phosphate (Gly-3-P) to GAPDH. To enhance the re...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-03, Vol.83 (5), p.1500-1503
Hauptverfasser:	Kim, Il Hong, Lee, Mi Nam, Ryu, Sung Ho, Park, Joon Won
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Analytical, structural and metabolic biochemistry Binding sites Biological and medical sciences Brain Cells Fundamental and applied biological sciences. Psychology Microscopy Microscopy, Atomic Force - methods Proteins Proteins - chemistry Signal Transduction
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