A high-throughput colorimetric-assay for monitoring glucose consumption by cultured trophoblast cells and placental tissue

Abstract Diabetes mellitus is associated with abnormalities in placental structure and function and significant pregnancy complications. In vitro studies to investigate the effect of hyperglycaemia on trophoblast structure and function require an accurate, inexpensive and reliable assay to monitor t...

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Veröffentlicht in:	Placenta (Eastbourne) 2012-11, Vol.33 (11), p.949-951
Hauptverfasser:	Hulme, C.H, Westwood, M, Myers, J.E, Heazell, A.E.P
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult BeWo cells Biological and medical sciences Biological Transport Cell Line Cells, Cultured Conditioned culture medium Culture Media, Conditioned - analysis Culture Media, Serum-Free - analysis Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Glucose Glucose - analysis Glucose - metabolism Glucose Oxidase - metabolism Horseradish Peroxidase - metabolism Humans Hyperglycemia - metabolism Indicators and Reagents - chemistry Internal Medicine Obstetrics and Gynecology Osmolar Concentration Placenta - metabolism Placental explants Pregnancy Spectrophotometry Time Factors Tissue Culture Techniques Trophoblasts - cytology Trophoblasts - metabolism
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