Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms
OBJECTIVE: Senescence of vascular cells contributes to the development of cardiovascular diseases and the overall aging. This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2012-11, Vol.23 (11), p.1410-1416 |
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| container_title | The Journal of nutritional biochemistry |
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| creator | Tang, Yuhan Xu, Jian Qu, Wei Peng, Xiaolin Xin, Peng Yang, Xuefeng Ying, Chenjiang Sun, Xiufa Hao, Liping |
| description | OBJECTIVE: Senescence of vascular cells contributes to the development of cardiovascular diseases and the overall aging. This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathway. METHODS AND RESULTS: Adult male Wistar rats were treated with a high-fat/sucrose diet (HFS) in the presence or absence of Res for 3 months. HFS and in vitro treatment with high glucose increased the senescence cells and reactive oxygen species production in rat aorta and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Res protected against HFS- or high-glucose-induced increase in NADPH oxidase p47phox expression and decrease in SIRT1 level. Apocynin, a NADPH oxidase inhibitor, down-regulated p47phox protein expression, but had no influence on SIRT1 protein; sirtinol, a SIRT1 inhibitor, aggravated the decrease in SIRT1 protein level and the increase in p47phox protein expression induced by high glucose. CONCLUSION: Our studies suggested that Res was able to reverse the senescence process in aorta induced by HFS in rats or induced by the exposure to high glucose in cultured BAECs. The underlying mechanism is at least SIRT1/NADPH oxidase pathway dependent. |
| doi_str_mv | 10.1016/j.jnutbio.2011.08.008 |
| format | Article |
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This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathway. METHODS AND RESULTS: Adult male Wistar rats were treated with a high-fat/sucrose diet (HFS) in the presence or absence of Res for 3 months. HFS and in vitro treatment with high glucose increased the senescence cells and reactive oxygen species production in rat aorta and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Res protected against HFS- or high-glucose-induced increase in NADPH oxidase p47phox expression and decrease in SIRT1 level. Apocynin, a NADPH oxidase inhibitor, down-regulated p47phox protein expression, but had no influence on SIRT1 protein; sirtinol, a SIRT1 inhibitor, aggravated the decrease in SIRT1 protein level and the increase in p47phox protein expression induced by high glucose. CONCLUSION: Our studies suggested that Res was able to reverse the senescence process in aorta induced by HFS in rats or induced by the exposure to high glucose in cultured BAECs. The underlying mechanism is at least SIRT1/NADPH oxidase pathway dependent.</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/j.jnutbio.2011.08.008</identifier><identifier>PMID: 22284404</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; aorta ; Aorta - cytology ; Aorta - drug effects ; Body Weight - drug effects ; cardiovascular diseases ; Cattle ; Cells, Cultured ; Cellular Senescence - drug effects ; Diet, High-Fat - adverse effects ; endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; glucose ; Glucose - adverse effects ; Lipids - blood ; Male ; NADP (coenzyme) ; NADPH oxidase ; NADPH Oxidases - metabolism ; oxidative stress ; Oxidative Stress - drug effects ; protein synthesis ; Rats ; Rats, Wistar ; reactive oxygen species ; Reactive Oxygen Species - metabolism ; Resveratrol ; senescence ; SIRT1 ; Sirtuin 1 - metabolism ; Stilbenes - pharmacology ; Vascular cell senescence</subject><ispartof>The Journal of nutritional biochemistry, 2012-11, Vol.23 (11), p.1410-1416</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jnutbio.2011.08.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22284404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tang, Yuhan</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><creatorcontrib>Qu, Wei</creatorcontrib><creatorcontrib>Peng, Xiaolin</creatorcontrib><creatorcontrib>Xin, Peng</creatorcontrib><creatorcontrib>Yang, Xuefeng</creatorcontrib><creatorcontrib>Ying, Chenjiang</creatorcontrib><creatorcontrib>Sun, Xiufa</creatorcontrib><creatorcontrib>Hao, Liping</creatorcontrib><title>Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>OBJECTIVE: Senescence of vascular cells contributes to the development of cardiovascular diseases and the overall aging. This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathway. METHODS AND RESULTS: Adult male Wistar rats were treated with a high-fat/sucrose diet (HFS) in the presence or absence of Res for 3 months. HFS and in vitro treatment with high glucose increased the senescence cells and reactive oxygen species production in rat aorta and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Res protected against HFS- or high-glucose-induced increase in NADPH oxidase p47phox expression and decrease in SIRT1 level. Apocynin, a NADPH oxidase inhibitor, down-regulated p47phox protein expression, but had no influence on SIRT1 protein; sirtinol, a SIRT1 inhibitor, aggravated the decrease in SIRT1 protein level and the increase in p47phox protein expression induced by high glucose. CONCLUSION: Our studies suggested that Res was able to reverse the senescence process in aorta induced by HFS in rats or induced by the exposure to high glucose in cultured BAECs. The underlying mechanism is at least SIRT1/NADPH oxidase pathway dependent.</description><subject>Animals</subject><subject>aorta</subject><subject>Aorta - cytology</subject><subject>Aorta - drug effects</subject><subject>Body Weight - drug effects</subject><subject>cardiovascular diseases</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence - drug effects</subject><subject>Diet, High-Fat - adverse effects</subject><subject>endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>glucose</subject><subject>Glucose - adverse effects</subject><subject>Lipids - blood</subject><subject>Male</subject><subject>NADP (coenzyme)</subject><subject>NADPH oxidase</subject><subject>NADPH Oxidases - metabolism</subject><subject>oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>protein synthesis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Resveratrol</subject><subject>senescence</subject><subject>SIRT1</subject><subject>Sirtuin 1 - metabolism</subject><subject>Stilbenes - pharmacology</subject><subject>Vascular cell senescence</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EotvCTwB85JLUn0n2hKqW0koVoH6cLceedL1K4sXjrOiNn06iXa6cZkbzaKR5H0I-cFZyxqvzbbkdp9yGWArGecmakrHmFVnxppaFalT9mqzYWutCNJU8IaeIW8aYULp6S06EEI1STK3In3vAPSSbU-xpAj85QLq36KbeJuqg7ynCCOhgdEDzJsXpeUNtzjBONoc40tjR-Dv4edgDxZwAkbYv9OH2_pGff7-4-nlz2CMUHnYwehgzHcBt7BhwwHfkTWd7hPfHekaerr8-Xt4Udz--3V5e3BUguc6F8Kpad0zVdVV3Uou6sq3jWjLfNmvZdXrdKgC-NJVg2utaa11VXuiGe6e4PCOfD3d3Kf6aALMZAi7_2RHihIZzrmompZYz-vGITu0A3uxSGGx6Mf9Sm4FPB6Cz0djnFNA8Pcwa1Jywkmo28D9CaKEW4suBgPnpfYBk0IUlZB8SuGx8DIYzs7g2W3N0bRbXhjVmdi3_AoDImls</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Tang, Yuhan</creator><creator>Xu, Jian</creator><creator>Qu, Wei</creator><creator>Peng, Xiaolin</creator><creator>Xin, Peng</creator><creator>Yang, Xuefeng</creator><creator>Ying, Chenjiang</creator><creator>Sun, Xiufa</creator><creator>Hao, Liping</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20121101</creationdate><title>Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms</title><author>Tang, Yuhan ; Xu, Jian ; Qu, Wei ; Peng, Xiaolin ; Xin, Peng ; Yang, Xuefeng ; Ying, Chenjiang ; Sun, Xiufa ; Hao, Liping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e315t-2d469f047767f35276abc1530db893ff59b4ee1ff596205d5755566d2581dc413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>aorta</topic><topic>Aorta - cytology</topic><topic>Aorta - drug effects</topic><topic>Body Weight - drug effects</topic><topic>cardiovascular diseases</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence - drug effects</topic><topic>Diet, High-Fat - adverse effects</topic><topic>endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>glucose</topic><topic>Glucose - adverse effects</topic><topic>Lipids - blood</topic><topic>Male</topic><topic>NADP (coenzyme)</topic><topic>NADPH oxidase</topic><topic>NADPH Oxidases - metabolism</topic><topic>oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>protein synthesis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Resveratrol</topic><topic>senescence</topic><topic>SIRT1</topic><topic>Sirtuin 1 - metabolism</topic><topic>Stilbenes - pharmacology</topic><topic>Vascular cell senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Yuhan</creatorcontrib><creatorcontrib>Xu, Jian</creatorcontrib><creatorcontrib>Qu, Wei</creatorcontrib><creatorcontrib>Peng, Xiaolin</creatorcontrib><creatorcontrib>Xin, Peng</creatorcontrib><creatorcontrib>Yang, Xuefeng</creatorcontrib><creatorcontrib>Ying, Chenjiang</creatorcontrib><creatorcontrib>Sun, Xiufa</creatorcontrib><creatorcontrib>Hao, Liping</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Yuhan</au><au>Xu, Jian</au><au>Qu, Wei</au><au>Peng, Xiaolin</au><au>Xin, Peng</au><au>Yang, Xuefeng</au><au>Ying, Chenjiang</au><au>Sun, Xiufa</au><au>Hao, Liping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>23</volume><issue>11</issue><spage>1410</spage><epage>1416</epage><pages>1410-1416</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>OBJECTIVE: Senescence of vascular cells contributes to the development of cardiovascular diseases and the overall aging. This study was undertaken to investigate the effects of resveratrol (Res) on amelioration of vascular cell aging and the role of SIRT1/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathway. METHODS AND RESULTS: Adult male Wistar rats were treated with a high-fat/sucrose diet (HFS) in the presence or absence of Res for 3 months. HFS and in vitro treatment with high glucose increased the senescence cells and reactive oxygen species production in rat aorta and cultured bovine aortic endothelial cells (BAECs), respectively, which was attenuated by Res treatment. Res protected against HFS- or high-glucose-induced increase in NADPH oxidase p47phox expression and decrease in SIRT1 level. Apocynin, a NADPH oxidase inhibitor, down-regulated p47phox protein expression, but had no influence on SIRT1 protein; sirtinol, a SIRT1 inhibitor, aggravated the decrease in SIRT1 protein level and the increase in p47phox protein expression induced by high glucose. CONCLUSION: Our studies suggested that Res was able to reverse the senescence process in aorta induced by HFS in rats or induced by the exposure to high glucose in cultured BAECs. The underlying mechanism is at least SIRT1/NADPH oxidase pathway dependent.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22284404</pmid><doi>10.1016/j.jnutbio.2011.08.008</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals aorta Aorta - cytology Aorta - drug effects Body Weight - drug effects cardiovascular diseases Cattle Cells, Cultured Cellular Senescence - drug effects Diet, High-Fat - adverse effects endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - drug effects glucose Glucose - adverse effects Lipids - blood Male NADP (coenzyme) NADPH oxidase NADPH Oxidases - metabolism oxidative stress Oxidative Stress - drug effects protein synthesis Rats Rats, Wistar reactive oxygen species Reactive Oxygen Species - metabolism Resveratrol senescence SIRT1 Sirtuin 1 - metabolism Stilbenes - pharmacology Vascular cell senescence |
| title | Resveratrol reduces vascular cell senescence through attenuation of oxidative stress by SIRT1/NADPH oxidase-dependent mechanisms |
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