Evaluation of the Dry Sheet Medium (Compact Dry ETBR) for Enumerating Enterobacteriaceae in Meat Samples
Compact Dry ETBR (CD-ETB, ETB; Enterobacteriaceae) , a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Enterobacteriaceae, was evaluated. A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-...
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| Veröffentlicht in: | Biocontrol Science 2012, Vol.17(3), pp.149-154 |
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| creator | TERAMURA, HAJIME MIZUOCHI, SHINGO KODAKA, HIDEMASA |
| description | Compact Dry ETBR (CD-ETB, ETB; Enterobacteriaceae) , a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Enterobacteriaceae, was evaluated. A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-purple colored colonies on the CD-ETBR. When 17 gram-negative bacteria other than Enterobacteriaceae were inoculated for the exclusivity study, 2 strains grew and formed red-purple colonies, 9 strains formed colorless colonies, and 6 strains failed to grow. A total of 43 gram-positive bacteria and 3 yeasts failed to grow. The CD-ETB method was compared with the Violet Red Bile Glucose (VRBG) agar method according to ISO 21528-2 and the 3M PetrifilmTM EB (3M-EBR) method in 53 meat samples. The correlation coefficients between CD-ETBR and VRBG agar, CD-ETBR and 3M-EBR, and 3M-EBR and VRBG agar were 0.962, 0.992 and 0.960, respectively. |
| doi_str_mv | 10.4265/bio.17.149 |
| format | Article |
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A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-purple colored colonies on the CD-ETBR. When 17 gram-negative bacteria other than Enterobacteriaceae were inoculated for the exclusivity study, 2 strains grew and formed red-purple colonies, 9 strains formed colorless colonies, and 6 strains failed to grow. A total of 43 gram-positive bacteria and 3 yeasts failed to grow. The CD-ETB method was compared with the Violet Red Bile Glucose (VRBG) agar method according to ISO 21528-2 and the 3M PetrifilmTM EB (3M-EBR) method in 53 meat samples. The correlation coefficients between CD-ETBR and VRBG agar, CD-ETBR and 3M-EBR, and 3M-EBR and VRBG agar were 0.962, 0.992 and 0.960, respectively.</description><identifier>ISSN: 1342-4815</identifier><identifier>EISSN: 1884-0205</identifier><identifier>DOI: 10.4265/bio.17.149</identifier><identifier>PMID: 23007107</identifier><language>eng</language><publisher>Japan: The Society for Antibacterial and Antifungal Agents, Japan</publisher><subject>Agar ; Bile ; Biological control ; Colonies ; Colony Count, Microbial - instrumentation ; Colony Count, Microbial - methods ; Compact Dry ETB ; Culture Media - metabolism ; Enterobacteriaceae ; Enterobacteriaceae - growth & development ; Enterobacteriaceae - metabolism ; Food Contamination - analysis ; Glucose ; Gram-negative bacteria ; Gram-positive bacteria ; Meat ; Meat - microbiology ; Ready-to-use medium</subject><ispartof>Biocontrol Science, 2012, Vol.17(3), pp.149-154</ispartof><rights>2012 The Society for Antibacterial and Antifungal Agents, Japan</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c422t-bdd7fa40812ff29ebfea84eba4255ec6d3a0c2a664a582134bdc7f9e404a9173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23007107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TERAMURA, HAJIME</creatorcontrib><creatorcontrib>MIZUOCHI, SHINGO</creatorcontrib><creatorcontrib>KODAKA, HIDEMASA</creatorcontrib><title>Evaluation of the Dry Sheet Medium (Compact Dry ETBR) for Enumerating Enterobacteriaceae in Meat Samples</title><title>Biocontrol Science</title><addtitle>Biocontrol Sci.</addtitle><description>Compact Dry ETBR (CD-ETB, ETB; Enterobacteriaceae) , a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Enterobacteriaceae, was evaluated. A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-purple colored colonies on the CD-ETBR. When 17 gram-negative bacteria other than Enterobacteriaceae were inoculated for the exclusivity study, 2 strains grew and formed red-purple colonies, 9 strains formed colorless colonies, and 6 strains failed to grow. A total of 43 gram-positive bacteria and 3 yeasts failed to grow. The CD-ETB method was compared with the Violet Red Bile Glucose (VRBG) agar method according to ISO 21528-2 and the 3M PetrifilmTM EB (3M-EBR) method in 53 meat samples. The correlation coefficients between CD-ETBR and VRBG agar, CD-ETBR and 3M-EBR, and 3M-EBR and VRBG agar were 0.962, 0.992 and 0.960, respectively.</description><subject>Agar</subject><subject>Bile</subject><subject>Biological control</subject><subject>Colonies</subject><subject>Colony Count, Microbial - instrumentation</subject><subject>Colony Count, Microbial - methods</subject><subject>Compact Dry ETB</subject><subject>Culture Media - metabolism</subject><subject>Enterobacteriaceae</subject><subject>Enterobacteriaceae - growth & development</subject><subject>Enterobacteriaceae - metabolism</subject><subject>Food Contamination - analysis</subject><subject>Glucose</subject><subject>Gram-negative bacteria</subject><subject>Gram-positive bacteria</subject><subject>Meat</subject><subject>Meat - microbiology</subject><subject>Ready-to-use medium</subject><issn>1342-4815</issn><issn>1884-0205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPwzAQhC0E4n3hByAfASnFdpzXgQOU8pBASNC7tXHWNCiJi-0g8e8xtPS0o91vRtoh5ISziRR5dlm3dsKLCZfVFtnnZSkTJli2HXUqRSJLnu2RA-8_GMvLssp2yZ5IGSs4K_bJYvYF3QihtQO1hoYF0lv3Td8WiIE-Y9OOPT2b2n4JOvxdZvOb13NqrKOzYezRRevwHnVAZ-sIoWtBIyBth-iHQN-gX3boj8iOgc7j8XoekvndbD59SJ5e7h-n10-JlkKEpG6awoBkJRfGiAprg1BKrEGKLEOdNykwLSDPJWSliA_WjS5MhZJJqHiRHpKzVezS2c8RfVB96zV2HQxoR68456ngRS5ZRC9WqHbWe4dGLV3bg_tWnKnfYlUsVvFCxWIjfLrOHesemw3632QErlbAhw_wjhsAXGh1h_9Z6Tpws9cLcAqH9Af664n_</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>TERAMURA, HAJIME</creator><creator>MIZUOCHI, SHINGO</creator><creator>KODAKA, HIDEMASA</creator><general>The Society for Antibacterial and Antifungal Agents, Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2012</creationdate><title>Evaluation of the Dry Sheet Medium (Compact Dry ETBR) for Enumerating Enterobacteriaceae in Meat Samples</title><author>TERAMURA, HAJIME ; MIZUOCHI, SHINGO ; KODAKA, HIDEMASA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-bdd7fa40812ff29ebfea84eba4255ec6d3a0c2a664a582134bdc7f9e404a9173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agar</topic><topic>Bile</topic><topic>Biological control</topic><topic>Colonies</topic><topic>Colony Count, Microbial - instrumentation</topic><topic>Colony Count, Microbial - methods</topic><topic>Compact Dry ETB</topic><topic>Culture Media - metabolism</topic><topic>Enterobacteriaceae</topic><topic>Enterobacteriaceae - growth & development</topic><topic>Enterobacteriaceae - metabolism</topic><topic>Food Contamination - analysis</topic><topic>Glucose</topic><topic>Gram-negative bacteria</topic><topic>Gram-positive bacteria</topic><topic>Meat</topic><topic>Meat - microbiology</topic><topic>Ready-to-use medium</topic><toplevel>online_resources</toplevel><creatorcontrib>TERAMURA, HAJIME</creatorcontrib><creatorcontrib>MIZUOCHI, SHINGO</creatorcontrib><creatorcontrib>KODAKA, HIDEMASA</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biocontrol Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TERAMURA, HAJIME</au><au>MIZUOCHI, SHINGO</au><au>KODAKA, HIDEMASA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the Dry Sheet Medium (Compact Dry ETBR) for Enumerating Enterobacteriaceae in Meat Samples</atitle><jtitle>Biocontrol Science</jtitle><addtitle>Biocontrol Sci.</addtitle><date>2012</date><risdate>2012</risdate><volume>17</volume><issue>3</issue><spage>149</spage><epage>154</epage><pages>149-154</pages><issn>1342-4815</issn><eissn>1884-0205</eissn><abstract>Compact Dry ETBR (CD-ETB, ETB; Enterobacteriaceae) , a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Enterobacteriaceae, was evaluated. A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-purple colored colonies on the CD-ETBR. When 17 gram-negative bacteria other than Enterobacteriaceae were inoculated for the exclusivity study, 2 strains grew and formed red-purple colonies, 9 strains formed colorless colonies, and 6 strains failed to grow. A total of 43 gram-positive bacteria and 3 yeasts failed to grow. The CD-ETB method was compared with the Violet Red Bile Glucose (VRBG) agar method according to ISO 21528-2 and the 3M PetrifilmTM EB (3M-EBR) method in 53 meat samples. The correlation coefficients between CD-ETBR and VRBG agar, CD-ETBR and 3M-EBR, and 3M-EBR and VRBG agar were 0.962, 0.992 and 0.960, respectively.</abstract><cop>Japan</cop><pub>The Society for Antibacterial and Antifungal Agents, Japan</pub><pmid>23007107</pmid><doi>10.4265/bio.17.149</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agar Bile Biological control Colonies Colony Count, Microbial - instrumentation Colony Count, Microbial - methods Compact Dry ETB Culture Media - metabolism Enterobacteriaceae Enterobacteriaceae - growth & development Enterobacteriaceae - metabolism Food Contamination - analysis Glucose Gram-negative bacteria Gram-positive bacteria Meat Meat - microbiology Ready-to-use medium |
| title | Evaluation of the Dry Sheet Medium (Compact Dry ETBR) for Enumerating Enterobacteriaceae in Meat Samples |
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