Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment

The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 4...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2012, Vol.76 (7), p.1372-1377
Hauptverfasser:	FUKUDA, Tomokazu, KATAYAMA, Masafumi, YOSHIZAWA, Takayuki, EITSUKA, Takahiro, MIZUKAMI, Hiroaki, NAKAGAWA, Kiyotaka, ITO, Hisao, KOMAGATA, Homika, SONG, Sanghoun, ROH, Sanggun, HOSHINO, Yumi, SATO, Eimei, HANADA, Hirofumi, NISHIMORI, Katsuhiko, MIYAZAWA, Teruo, UCHIDA, Takafumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Polyomavirus Transforming - genetics Biochemistry Biological and medical sciences Biotechnology Cell Line Cell Proliferation Chromosomes conditional expression system Embryo, Mammalian Fibroblasts Fibroblasts - cytology Fibroblasts - metabolism Fibroblasts - virology Founder Effect Fragmentation Fundamental and applied biological sciences. Psychology Gene Expression Genetic Engineering Genomes immortalization Integrases - genetics Karyotype Karyotyping retrovirus Simian vacuolating virus 40 large T fragment (SV40T) Simian virus 40 - genetics Swine
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