Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment
The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 4...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2012, Vol.76 (7), p.1372-1377 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | FUKUDA, Tomokazu KATAYAMA, Masafumi YOSHIZAWA, Takayuki EITSUKA, Takahiro MIZUKAMI, Hiroaki NAKAGAWA, Kiyotaka ITO, Hisao KOMAGATA, Homika SONG, Sanghoun ROH, Sanggun HOSHINO, Yumi SATO, Eimei HANADA, Hirofumi NISHIMORI, Katsuhiko MIYAZAWA, Teruo UCHIDA, Takafumi |
| description | The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals. |
| doi_str_mv | 10.1271/bbb.120155 |
| format | Article |
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However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.120155</identifier><identifier>PMID: 22785463</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; Antigens, Polyomavirus Transforming - genetics ; Biochemistry ; Biological and medical sciences ; Biotechnology ; Cell Line ; Cell Proliferation ; Chromosomes ; conditional expression system ; Embryo, Mammalian ; Fibroblasts ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Fibroblasts - virology ; Founder Effect ; Fragmentation ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic Engineering ; Genomes ; immortalization ; Integrases - genetics ; Karyotype ; Karyotyping ; retrovirus ; Simian vacuolating virus 40 large T fragment (SV40T) ; Simian virus 40 - genetics ; Swine</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2012, Vol.76 (7), p.1372-1377</ispartof><rights>2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2012</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-e4a3290c62fd82b6f30316974269cb3683923e3b698410ce54f7369c669c44613</citedby><cites>FETCH-LOGICAL-c547t-e4a3290c62fd82b6f30316974269cb3683923e3b698410ce54f7369c669c44613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26419781$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22785463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FUKUDA, Tomokazu</creatorcontrib><creatorcontrib>KATAYAMA, Masafumi</creatorcontrib><creatorcontrib>YOSHIZAWA, Takayuki</creatorcontrib><creatorcontrib>EITSUKA, Takahiro</creatorcontrib><creatorcontrib>MIZUKAMI, Hiroaki</creatorcontrib><creatorcontrib>NAKAGAWA, Kiyotaka</creatorcontrib><creatorcontrib>ITO, Hisao</creatorcontrib><creatorcontrib>KOMAGATA, Homika</creatorcontrib><creatorcontrib>SONG, Sanghoun</creatorcontrib><creatorcontrib>ROH, Sanggun</creatorcontrib><creatorcontrib>HOSHINO, Yumi</creatorcontrib><creatorcontrib>SATO, Eimei</creatorcontrib><creatorcontrib>HANADA, Hirofumi</creatorcontrib><creatorcontrib>NISHIMORI, Katsuhiko</creatorcontrib><creatorcontrib>MIYAZAWA, Teruo</creatorcontrib><creatorcontrib>UCHIDA, Takafumi</creatorcontrib><title>Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>The pig is an important animal for both agricultural and medical purposes. However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.</description><subject>Animals</subject><subject>Antigens, Polyomavirus Transforming - genetics</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Chromosomes</subject><subject>conditional expression system</subject><subject>Embryo, Mammalian</subject><subject>Fibroblasts</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - virology</subject><subject>Founder Effect</subject><subject>Fragmentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic Engineering</subject><subject>Genomes</subject><subject>immortalization</subject><subject>Integrases - genetics</subject><subject>Karyotype</subject><subject>Karyotyping</subject><subject>retrovirus</subject><subject>Simian vacuolating virus 40 large T fragment (SV40T)</subject><subject>Simian virus 40 - genetics</subject><subject>Swine</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-KEzEUxoMobl298QEkIIII1fybZOZyKVMVCgquezskadJmySQ1ybD2JXxmM7SrIMJehOQkv_Odk_MB8BKj95gI_EEpVQ8IN80jsMCUiSXvmHgMFqjDfNmyBl-AZznfIlQvGvwUXBAi2oZxugC_emuddiYU2OcilXd5P85RtPCr28F-VOkYg9Nw7VSKystc4Mp4DzcumAzvXNnDVQxbV1wM0sP-5yGZnGswS5S9gd_c6GSAN1JP0cviwg7euDRlyBDcyLQz8Bquk9zNZZ-DJ1b6bF6c90vwfd1frz4tN18-fl5dbZa6YaIsDZOUdEhzYrctUdxSRDHvBCO804rylnaEGqp41zKMtGmYFbQ-8boY45hegrcn3UOKPyaTyzC6rOu3ZDBxygPmAtcETsXDKMaUYFGn_DCKiOAt4ZRU9PU_6G2cUh1gpRjr2oYINgu-O1E6xZyTscMhuVGmY5UaZu-H6v1w8r7Cr86SkxrN9g96b3YF3pwBmbX0NsmgXf7LcYY70c5VmxPngo1plHcx-e1Q5NHHdJ9E_9PAbyB3xjg</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>FUKUDA, Tomokazu</creator><creator>KATAYAMA, Masafumi</creator><creator>YOSHIZAWA, Takayuki</creator><creator>EITSUKA, Takahiro</creator><creator>MIZUKAMI, Hiroaki</creator><creator>NAKAGAWA, Kiyotaka</creator><creator>ITO, Hisao</creator><creator>KOMAGATA, Homika</creator><creator>SONG, Sanghoun</creator><creator>ROH, Sanggun</creator><creator>HOSHINO, Yumi</creator><creator>SATO, Eimei</creator><creator>HANADA, Hirofumi</creator><creator>NISHIMORI, Katsuhiko</creator><creator>MIYAZAWA, Teruo</creator><creator>UCHIDA, Takafumi</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7TB</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>2012</creationdate><title>Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment</title><author>FUKUDA, Tomokazu ; KATAYAMA, Masafumi ; YOSHIZAWA, Takayuki ; EITSUKA, Takahiro ; MIZUKAMI, Hiroaki ; NAKAGAWA, Kiyotaka ; ITO, Hisao ; KOMAGATA, Homika ; SONG, Sanghoun ; ROH, Sanggun ; HOSHINO, Yumi ; SATO, Eimei ; HANADA, Hirofumi ; NISHIMORI, Katsuhiko ; MIYAZAWA, Teruo ; UCHIDA, Takafumi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-e4a3290c62fd82b6f30316974269cb3683923e3b698410ce54f7369c669c44613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antigens, Polyomavirus Transforming - genetics</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Chromosomes</topic><topic>conditional expression system</topic><topic>Embryo, Mammalian</topic><topic>Fibroblasts</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - virology</topic><topic>Founder Effect</topic><topic>Fragmentation</topic><topic>Fundamental and applied biological sciences. 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However, the number of pig-derived cell lines is relatively limited when compared with mouse- and human-derived lines. We established in this study a retroviral conditional expression system for the Simian vacuolating virus 40 large T fragment (SV40T) which allowed us to efficiently establish pig embryonic fibroblast cell lines. The established cell lines showed high levels of cell proliferation and resistance to cellular senescence. A chromosome analysis showed that 84% of the cells had the normal karyotype. Transient expression of the Cre recombinase allowed us to excise the SV40T fragment from the genome. The development of this research tool will enable us to quickly establish new cell lines derived from various animals.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>22785463</pmid><doi>10.1271/bbb.120155</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2012, Vol.76 (7), p.1372-1377 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | Freely Accessible Japanese Titles (ERDB Project); MEDLINE; Full-Text Journals in Chemistry (Open access); Oxford University Press; EZB Electronic Journals Library; J-STAGE |
| subjects | Animals Antigens, Polyomavirus Transforming - genetics Biochemistry Biological and medical sciences Biotechnology Cell Line Cell Proliferation Chromosomes conditional expression system Embryo, Mammalian Fibroblasts Fibroblasts - cytology Fibroblasts - metabolism Fibroblasts - virology Founder Effect Fragmentation Fundamental and applied biological sciences. Psychology Gene Expression Genetic Engineering Genomes immortalization Integrases - genetics Karyotype Karyotyping retrovirus Simian vacuolating virus 40 large T fragment (SV40T) Simian virus 40 - genetics Swine |
| title | Efficient Establishment of Pig Embryonic Fibroblast Cell Lines with Conditional Expression of the Simian Vacuolating Virus 40 Large T Fragment |
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